Dr. Liao, You-Di 廖有地 博士

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Dr. Liao, You-Di
廖有地 博士

Research Fellow
研究員

Specialty:
  • Protein Chemistry
  • Antitumor Ribonucleases
  • Antimicrobial Protein/Peptides

RESEARCH

 1. Ribonucleases with antitumor activity are mainly found in the oocytes and embryos of Rana catesbeiana (bullfrog), but the mechanism of antitumor activity and their roles in frog development are not clear. The residues involved in catalytic activity base recognition and cytotoxicity are investigated by site-directed mutagenesis, NMR and X-ray cocrystallography.

 

2. Antimicrobial peptides/proteins (AMPs) are important components ofthe host innate defense mechanisms against invading microorganisms.The human ribonuclease 7(hRNase 7) possesses membrane permeability and antimicrobial activity through the flexible and clustered lysine residues near N-terminus. In addition to hRNase 7, hundreds of AMPs also exert similar antimicrobial activity. The susceptibility of Gram negative bacteria to these AMPs is determined by an outer membrane protein, like the OprI of Pseudomonas aeruginosa and Lpp of E. coli. Therefore, OprI and its homologues may be used as ligands for the screening and production of antimicrobial materials.

 

3. The removal of N-terminal Met is often crucial for the function and stability of recombinant proteins. We engineer an E. coli methionine aminopeptidase (MetAP) which is able to remove N-terminal Met from bulky or acidic penultimate residues.

研究介紹

1. 牛蛙之RNA 水解酵素群具抗癌特性,本實驗室以基因突變,核磁共振及X光繞射法,研究其抗癌機制及生化特性。

 

2. RNA 水解酵素及陽離子抗菌胜肽扮演宿主防禦重要的角色,本實驗室發現其參與破壞細菌外膜的胺基酸,進而找到其在細菌外膜之標的蛋白質,藉此研究抗菌機轉及篩選抗菌胜肽,以克服抗藥菌株產生的問題。

 

3. 甲硫胺酸(Met)為蛋白質合成之起始胺基酸,但常會影響蛋白質之功能及穩定性,我們改造了大腸桿菌之甲硫胺酸去除酶,應用於大腸桿菌表現重組蛋白質,可去除蛋白質N端的甲硫胺酸。

HIGHLIGHT 重要成果

CREW PHOTO 成員合照

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