M.D. National Taiwan University, Ph.D. National Cheng Kung University
The goal of our laboratory is to explore the genetic regulation, immunological development, prognostic factors and pharmacogenetics in allergic diseases. We have tried to identify novel loci of dermatitis and asthma and to use the molecular biology approaches to understand mechanisms underlying the associations. We hope these works could be applied in clinical settings for improving personalized prevention and treatment protocols of allergic diseases.
Our current research projects include:
1. Dendritic cells (DCs) are a major group of antigen presenting cells that mediate immunological responses confer by leukocytes in response to alien or self antigens. They play an integral part in maintaining homeostasis and development of skin immunology through their abilities to train, activate, as well as influence T cells developments and functions. Our laboratory is interested in the roles that specific DC subsets play in the pathology and, perhaps, pathogenesis of atopic skin allergic diseases, such as atopic dermatitis (AD). We are particularly fascinated by the roles that these cells play in mediating skin inflammation via their interactions with other immune cells. Using our AD-like murine model in combination with several lines of gene knock-out mice and in vitro cell cultures, we are currently investigating specific DC subsets as potential targets that are directly affected by bacterial-source antigens. The aim is to elucidate the roles of DCs in meditating and directing AD phenotypes exacerbated by Staphylococcus aureus infection. We also aim to gain a better understanding of the cellular as well as the underlying molecular pathways driving DCs and their interactions with other skin immune cells in the context of AD.
2. To study roles of skin in immunological function. Keratinocytes provide the physical barrier against outside pathogens, protect the body by producing anti-microbial peptides, and interact with immune cells. We are interested in the interactions between skin and immune system and their relationships with skin inflammation and skin allergy.
3. To understand whether glycosylation process regulates the interactions between skin and immune system, and whether glycosylation process would influence the pathogenesis of skin inflammation and skin allergy. We have applied biochip analysis in skin tissues from patients with AD, psoriasis or contact dermatitis. Based on these results, we have chosen some glycosyl-transferases, such as FuT8, as study targets. Besides, we also investigate the functional roles of the downstream glycoproteins in the skin inflammatory mechanisms.
1.Dendritic cells是皮膚主要的抗原呈現細胞，負責維持皮膚的恆定與T細胞個活化與功能。我們探討特殊DC族群，對於皮膚發炎反應與異位性皮膚炎(Atopic Dermatitis, AD)發生的影響，另用AD動物模型與knock-out mice，我們研究多種病原抗原引起皮膚發炎反應的機轉，同時，我們也試圖瞭解啟動DC的細胞分子路徑，以及接續的皮膚免疫反應。