Dr. Lee, Yungling Leo李永凌 博士

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Dr. Lee, Yungling Leo
李永凌 博士

Joint Appointment Associate Research Fellow
合聘副研究員

  • M.D. National Taiwan University, Ph.D. National Cheng Kung University

  • 2789-9132 (lab) (Lab) (Room No: 343)
  • 02-26523013 (office); 3366-8016 (NTU)
  • 02-27829142 (Fax)
Specialty:
  • Skin Inflammation
  • Glyco Medicine
  • Pharmacogenetics

RESEARCH

The goal of our laboratory is to explore the genetic regulation, immunological development, prognostic factors and pharmacogenetics in allergic diseases. We have tried to identify novel loci of dermatitis and asthma and to use the molecular biology approaches to understand mechanisms underlying the associations. We hope these works could be applied in clinical settings for improving personalized prevention and treatment protocols of allergic diseases.

Our current research projects include:

1. Dendritic cells (DCs) are a major group of antigen presenting cells that mediate immunological responses confer by leukocytes in response to alien or self antigens. They play an integral part in maintaining homeostasis and development of skin immunology through their abilities to train, activate, as well as influence T cells developments and functions. Our laboratory is interested in the roles that specific DC subsets play in the pathology and, perhaps, pathogenesis of atopic skin allergic diseases, such as atopic dermatitis (AD). We are particularly fascinated by the roles that these cells play in mediating skin inflammation via their interactions with other immune cells. Using our AD-like murine model in combination with several lines of gene knock-out mice and in vitro cell cultures, we are currently investigating specific DC subsets as potential targets that are directly affected by bacterial-source antigens. The aim is to elucidate the roles of DCs in meditating and directing AD phenotypes exacerbated by Staphylococcus aureus infection. We also aim to gain a better understanding of the cellular as well as the underlying molecular pathways driving DCs and their interactions with other skin immune cells in the context of AD.

2. To study roles of skin in immunological function. Keratinocytes provide the physical barrier against outside pathogens, protect the body by producing anti-microbial peptides, and interact with immune cells. We are interested in the interactions between skin and immune system and their relationships with skin inflammation and skin allergy.

3. To understand whether glycosylation process regulates the interactions between skin and immune system, and whether glycosylation process would influence the pathogenesis of skin inflammation and skin allergy. We have applied biochip analysis in skin tissues from patients with AD, psoriasis or contact dermatitis. Based on these results, we have chosen some glycosyl-transferases, such as FuT8, as study targets. Besides, we also investigate the functional roles of the downstream glycoproteins in the skin inflammatory mechanisms.

研究介紹

本實驗室致力於探索過敏疾病的發生、免疫進展、預後及藥物基因體學。我們找出與皮膚炎、氣喘有關的基因,並利用分子免疫學等研究平台來瞭解相關分子機轉,期望可以找出有臨床應用價值的分子,逐步邁向過敏疾病個人化治療與預防的終極目標。

目前我們重點研究的方向有:

1.Dendritic cells是皮膚主要的抗原呈現細胞,負責維持皮膚的恆定與T細胞個活化與功能。我們探討特殊DC族群,對於皮膚發炎反應與異位性皮膚炎(Atopic Dermatitis, AD)發生的影響,另用AD動物模型與knock-out mice,我們研究多種病原抗原引起皮膚發炎反應的機轉,同時,我們也試圖瞭解啟動DC的細胞分子路徑,以及接續的皮膚免疫反應。

2.研究皮膚在免疫功能上扮演的重要角色。表皮中主要組成的角質細胞(keratinocyte)在結構上能阻隔病源菌、產生抗菌蛋白,還能與免疫細胞作用,我們探討這角質細胞與免疫系統的交互作用、以及皮膚發炎與過敏之間的關係。

3.探討醣基化(glycosylation)過程調控皮膚與免疫系統,以及是否和皮膚發炎與過敏的致病機轉有關。我們利用異位性皮膚炎、乾癬、接觸性皮膚炎患者皮膚切片檢體進行生物晶片分析,目前挑選FuT8等醣化酵素作為研究標的,另外,我們也探討皮膚發炎反應路徑中相關醣蛋白(glycoprotein)醣基化程度,與其分子訊號傳遞之間的關聯。

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