AGP3 / E11 - Anti-polyethelene glycol monoclonal antibodies AGP3 - Anti-polyethelene glycol monoclonal antibody


We have generated a monoclonal antibody that binds to the backbone of polyethelene glycol (PEG). This antibody is an IgM and is very specific for PEG. It can be employed to detect pegylated proteins in ELISA and Western blots. This antibody is very useful for measuring the pharmacokinetics of PEG-modified molecules in vivo. We have also generated an IgG monoclonal antibody against PEG (E11). These antibodies are currently being used by over 50 companies.

 

Antibody purchasing information

 

General notes:

1. AGP3 is an IgM antibody so it is better to use anti-IgM specific secondary detection reagents.

2. Both AGP3 and E11 bind to the PEG backbone. This means they bind to all forms of PEG. Also, assay sensitivity increases with PEG chain number and length.

3. AGP3 and E11 can bind to PEG modified proteins, liposomes and nanoparticles. Probably to anything that incorporates PEG chains. Please note, however, that neither AGP3 nor E11 work well for assays that measure "free" PEG. The PEG chains need to be mobilized to a substance, even to a plastic plate (for example, amino-PEG coated microtiter plates).

4. In our hands, the best assay sensitivity is achieved by coating AGP3 and detecting with AGP3-biotin      +     streptavidin-detector

5. We have many lots of both AGP3 and E11 available. We are continuously producing additional lots. If you need to reserve a particular lot, just let us know and we will do our best to meet your needs.

6. If you are a researcher in a non-profit institute or university, I can send you some antibody for your experiments free of charge.

 

 

Some Protocols. These are suggestions -- optimal conditions may depend on your pegylated compound

 

 

 

 

 

 

 

 

 

 

For more information, you can contact me (Steve Roffler) at sroff@ibms.sinica.edu.tw



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Related references

TL Cheng, CM Cheng, BM Chen, DA Tsao, KH Chuang, SW Hsiao, YH Lin and SR Roffler pdf
Monoclonal antibody-based quantitation of poly(ethylene glycol)-derivatized proteins, liposomes, and nanoparticles
Bioconj. Chem. 16:1225-31, 2005

NM Tsai, TL Cheng and SR Roffler pdf
Sensitive quantitation of poly(ethylene glycol)-modified proteins
Biotechniques 30: 396-402, 2001.

P Bailon, A Palleroni, CA Schaffer, CL Spence, WJ Fung, JE Porter, GK Ehrlich, W Pan, ZX Xu, M W Modi, A Farid and W Berthold pdf
Rational Design of a Potent, Long-Lasting Form of Interferon: A 40 kDa Branched Polyethylene Glycol-Conjugated Interferon r-2a for the Treatment of Hepatitis
Bioconjugate Chem. 2001, 12, 195-202

Cheng TL, Chen BM, Chern JW, Wu MF and Roffler SR. pdf
Efficient clearance of poly(ethylene glycol)-modified immunoenzyme with anti-PEG monoclonal antibody for prodrug cancer therapy
Bioconj. Chem., 11: 258-266, 2000.

Cheng TL, Wu PY, Wu MF, Chern JW, and Roffler SR. pdf
Accelerated clearance of polyethylene glycol-modified proteins by anti-polyethylene glycol IgM.
Bioconj. Chem. 10: 520-528, 1999.



Keywords: PEG polyethylene glycol polyethyleneglycol poly(ethylene) glycol pegylate pegylated antibody monoclonal antibody elisa immunoblot western blot anti-PEG anti-polyethylene glycol anti-poly ethylene glycol anti-poly(ethylene) glycol  AGP3  E11 hybridoma methoxy mPEG enzyme linked immunosorbent assay flow cytometer FACs IgG IgM immunoglobulin

 

 

Dr. Steve Roffler
Room N233
Institute of Biomedical Sciences

Academia Road, Section 2, No. 128
Academia Sinica
Taipei 11529, Taiwan

886-2-2652-3079