Product No. 3.3-PABG-B
3.3-biotin Data Sheet
| Description: | Mouse anti-PEG |
|---|---|
| Specificity: | Polyethylene glycol |
| Other names: | PEG |
| Format: | Purified |
| Product Type: | Monoclonal Antibody |
| Clone: | 3.3 |
| Isotype: | IgG1 |
| Species: | mouse |
| Price: | see price list |
3.3-biotin Product details
3.3-biotin is a second generation IgG1 anti-PEG monoclonal antibody. This antibody displays high affinity for the PEG backbone and can be employed as a detection antibody for the quantification of PEG.
Applications
This product has been reported to work in the following applications, The information is derived from experiments performed in our laboratory as well as from personal communications.
| Yes | No | Not Determined | |
| ELISA |
g |
||
| Western blotting | g | ||
| Flow cytometry | g | ||
| PEGylated liposomes | g | ||
| Immunohistochemistry | g | ||
| PEGylated quantum dots | g | ||
| Free PEG | † |
† free PEG can be detected onimmunoblots and if coated in microtiter plates. Free PEG is detected poorly by ELISA.
| Product Form | Purified IgG liquid |
|---|---|
| Preparation | Purified IgG prepared by affinity chromatography |
| Buffer solution | 50% glycerol in phosphate buffered saline pH 7.4 |
| Preservative | 0.02 % sodium azide (NaN3) |
| Immunogen | Conjugate of an antibody and PEG-modified E. coli β-glucuronidase |
| Fusion partners | Spleen cells from immunized BALB/c mice and FO myeloma cells |
|
Specificity |
3.3 binds to the repeating subunits of polyethylene glycol. The minimum PEG size that can be bound is ~ 2000 Da. Effective binding is greatly enhanced in PEG conjugates (i.e. PEG-protein, PEG-liposome, PEG-cell, PEG-nanoparticle conjugates). |
| ELISA |
Concentrations of 1-5 µg/ml (50 µl/well) are recommended. |
| Storage |
Store aliquots at -20oC. Antibodies should be stored undiluted. Avoid repeated freeze-thaw cycles. |
pdf of sample 3.3-biotin data sheet
Sandwich ELISA protocols
AGP3 capture ----- 3.3-biotin detection
AGP4 capture --- 3.3-biotin detection
3.3 Experimental data
AGP4 coating/ 3.3-biotin detection of Q-dots
Anti-PEG antibody AGP4 (0.25 mg/well) capture antibody
5 mg/ml 3.3-biotin, then 1 mg/ml streptavidin-HRP for detection
AGP4 coating / 3.3-biotin detection of Lipodox
Anti-PEG antibody AGP4 (0.25 mg/well) capture antibody
5 mg/ml 3.3-biotin, then 1 mg/ml streptavidin-HRP for detection
AGP4 coating/ 3.3-biotin detection of PEGasys
Anti-PEG antibody AGP4 (0.25 mg/well) capture antibody
5 mg/ml 3.3-biotin, then 1 mg/ml streptavidin-HRP for detection
AGP4 coating/ 3.3-biotin detection of Intron
Anti-PEG antibody AGP4 (0.25 mg/well) capture antibody
5 mg/ml 3.3-biotin, then 1 mg/ml streptavidin-HRP for detection
AGP3 coating/ 3.3-biotin detection of Lipodox
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
5 mg/ml 3.3-biotin detection then 1 mg/ml streptavidin-HRP
AGP3 coating/ 3.3-biotin detection of pegasys
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
5 mg/ml 3.3-biotin detection then 1 mg/ml streptavidin-HRP
AGP3 coating/ 3.3-biotin detection of Q-dots
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
5 mg/ml 3.3-biotin detection then 1 mg/ml streptavidin-HRP
Purchases can be made by contacting:
Ms. Mei Lee