Product No. AGP3-PABM-A
AGP3 Data Sheet
| Description: | Mouse anti-PEG |
|---|---|
| Specificity: | Polyethylene glycol |
| Other names: | PEG |
| Format: | Purified |
| Product Type: | Monoclonal Antibody |
| Clone: | AGP3 |
| Isotype: | IgM |
| Species: | mouse |
| Price: | see price list |
AGP3 Product details
AGP3 was the first monoclonal antibody developed with specificity for the repeating subunits present in PEG. This antibody of the IgM class is widely used in the pharmaceutical and biotechnology industries for quantification of PEG. A sensitive ELISA can be achieved utilizing AGP3 as a capture antibody and AGP3-biotin as the detection reagent.
Applications
This product has been reported to work in the following applications, The information is derived from experiments performed in our laboratory as well as from personal communications.
| Yes | No | Not Determined | |
| ELISA |
g |
||
| Western blotting | g | ||
| Flow cytometry | g | ||
| PEGylated liposomes | g | ||
| Immunohistochemistry | g | ||
| PEGylated quantum dots | g | ||
| Free PEG | † |
† free PEG can be detected onimmunoblots and if coated in microtiter plates. Free PEG is detected poorly by ELISA.
| Product Form | Purified IgM liquid |
|---|---|
| Preparation | Purified IgM prepared by gel filtration chromatography |
| Buffer solution | Phosphate buffered saline pH 7.4 |
| Preservative | 0.02% sodium azide (NaN3) |
| Immunogen | Conjugate of an antibody and PEG-modified E. coli β-glucuronidase |
| Fusion partners | Spleen cells from immunized BALB/c mice and FO myeloma cells |
|
Specificity |
AGP3 binds to the repeating subunits of polyethylene glycol. The minimum PEG size that can be bound is ~ 2000 Da. Effective binding is greatly enhanced in PEG conjugates (i.e. PEG-protein, PEG-liposome, PEG-cell, PEG-nanoparticle conjugates). |
| ELISA |
Coating concentrations of 10-20 µg/ml (50 µl/well) are recommended. AGP3-biotin, 3.3-biotin or AGP4-biotin are recommended for detection |
| Storage |
Store aliquots at -20oC. Antibodies should be stored undiluted. Avoid repeated freeze-thaw cycles. |
Sandwich ELISA Protocols
Anti-PEG antibody AGP3 as capture antibody ---- AGP3-biotin or AGP4-biotin detection
Anti-PEG antibody AGP3 as capture antibody ---- 3.3-biotin detection
AGP3 anti-PEG Experimental data
3.3-biotin detection of Lipodox
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
5 mg/ml 3.3-biotin detection then 1 mg/ml streptavidin-HRP
3.3-biotin detection of PEGasys
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
5 mg/ml 3.3-biotin detection then 1 mg/ml streptavidin-HRP
3.3-biotin detection of Q-dots
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
5 mg/ml 3.3-biotin detection then 1 mg/ml streptavidin-HRP
AGP3-biotin detection of Lipo-dox
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
Pre-mix 5 mg/ml AGP3-biotin & 1 mg/ml streptavidin-HRP detection
AGP3-biotin detection of PEGasys
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
Pre-mix 5 mg/ml AGP3-biotin & 1 mg/ml streptavidin-HRP detection
AGP3-biotin detection of Q-dots
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
Pre-mix 5 mg/ml AGP3-biotin & 1 mg/ml streptavidin-HRP detection
AGP4-biotin detection of Lipo-dox
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
Pre-mix 5 mg/ml AGP3-biotin & 1 mg/ml streptavidin-HRP detection
AGP4-biotin detection of pegasys
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
Pre-mix 5 mg/ml AGP3-biotin & 1 mg/ml streptavidin-HRP detection
AGP4-biotin detection of Q-dots
Anti-PEG antibody AGP3 (1 mg/well) capture antibody
Pre-mix 5 mg/ml AGP3-biotin & 1 mg/ml streptavidin-HRP detection
AGP3 experimental data is also available here:
TL
Cheng, CM Cheng, BM Chen, DA Tsao, KH Chuang, SW Hsiao, YH Lin and SR Roffler.
Monoclonal antibody-based quantitation of poly(ethylene glycol)-derivatized
proteins, liposomes, and nanoparticles. Bioconj. Chem. 16:1225-31, 2005.
(ELISA measurement of PEGylated compounds with Anti-PEG antibodies AGP3 and E11)
NM
Tsai, TL Cheng and SR Roffler. Sensitive quantitation of poly(ethylene
glycol)-modified proteins. Biotechniques 30: 396-402, 2001.
(Anti-PEG antibody AGP3 ELISA)
Purchases can be made by contacting:
Ms. Mei Lee