AGP4-biotin anti-PEG monoclonal antibody

Product No. AGP4-PABM-B

AGP4-biotin Data Sheet

Description:	Mouse anti-PEG
Specificity:	Polyethylene glycol
Other names:	PEG
Format:	Purified
Product Type:	Monoclonal Antibody
Clone:	AGP4
Isotype:	IgM
Species:	mouse
Price:	see price list

AGP4-biotin Product details

AGP4-biotin is a biotinylated IgM antibody that can be used to detect PEGylated compounds. A sensitive ELISA can be achieved utilizing AGP3, E11, or AGP4 as capture antibodies and AGP4-biotin as the detection reagent.

Applications

This product has been reported to work in the following applications, The information is derived from experiments performed in our laboratory as well as from personal communications.

	Yes	No	Not Determined
ELISA	g
Western blotting	g
Flow cytometry			g
PEGylated liposomes	g
Immunohistochemistry			g
PEGylated quantum dots	g
Free PEG	†

† free PEG can be detected on immunoblots and if coated in microtiter plates. Free PEG is detected poorly by ELISA.

Product Form	Purified IgM liquid
Preparation	Purified IgM prepared by gel filtration chromatography
Buffer solution	50% glycerol in phosphate buffered saline pH 7.4
Preservative	0.02% sodium azide (NaN₃)
Immunogen	Conjugate of an antibody and PEG-modified E. coli β-glucuronidase
Fusion partners	Spleen cells from immunized BALB/c mice and FO myeloma cells
Specificity	AGP4-biotin binds to the repeating subunits of polyethylene glycol. The minimum PEG size that can be bound is ~ 2000 Da. Effective binding is greatly enhanced in PEG conjugates (i.e. PEG-protein, PEG-liposome, PEG-cell, PEG-nanoparticle conjugates).
ELISA	Concentrations of 1-5 µg/ml are recommended.
Storage	Store aliquots at -20^oC or -80^oC. Antibodies should be stored undiluted. Avoid repeated freeze-thaw cycles. For long-term storage of working stocks, we suggest adding glycerol to 50% (vov/vol) to undiluted antibody and storing at -20^oC.