Product No. E11-PABG-A
E11 Data Sheet
| Description: | Mouse anti-PEG |
|---|---|
| Specificity: | Polyethylene glycol |
| Other names: | PEG |
| Format: | Purified |
| Product Type: | Monoclonal Antibody |
| Clone: | E11 |
| Isotype: | IgG1 |
| Species: | mouse |
| Price: | see price list |
E11 Product details
AGP3 was the first monoclonal antibody developed with specificity for the repeating subunits present in PEG. This antibody of the IgM class is widely used in the pharmaceutical and biotechnology industries for quantification of PEG. A sensitive ELISA can be achieved utilizing E11 as a capture antibody and AGP3-biotin or AGP4-biotin as the detection reagent.
Applications
This product has been reported to work in the following applications, The information is derived from experiments performed in our laboratory as well as from personal communications.
| Yes | No | Not Determined | |
| ELISA |
g |
||
| Western blotting | g | ||
| Flow cytometry | g | ||
| PEGylated liposomes | g | ||
| Immunohistochemistry | g | ||
| PEGylated quantum dots | g | ||
| Free PEG | † |
† free PEG can be detected onimmunoblots and if coated in microtiter plates. Free PEG is detected poorly by ELISA.
| Product Form | Purified IgG liquid |
|---|---|
| Preparation | Purified IgG prepared by affinity chromatography |
| Buffer solution | Phosphate buffered saline pH 7.4 |
| Preservative | 0.02% sodium azide (NaN3) |
| Immunogen | Conjugate of an antibody and PEG-modified E. coli β-glucuronidase |
| Fusion partners | Spleen cells from immunized BALB/c mice and FO myeloma cells |
|
Specificity |
E11 binds to the repeating subunits of polyethylene glycol. The minimum PEG size that can be bound is ~ 2000 Da. Effective binding is greatly enhanced in PEG conjugates (i.e. PEG-protein, PEG-liposome, PEG-cell, PEG-nanoparticle conjugates). |
| ELISA |
Coating concentrations of 10-20 µg/ml (50 µl/well) are recommended. AGP3-biotin, 3.3-biotin or AGP4-biotin are recommended for detection |
| Storage |
Store aliquots at -20oC. Antibodies should be stored undiluted. Avoid repeated freeze-thaw cycles. |
E11 Experimental data
TL
Cheng, CM Cheng, BM Chen, DA Tsao, KH Chuang, SW Hsiao, YH Lin and SR Roffler.
Monoclonal antibody-based quantitation of poly(ethylene glycol)-derivatized
proteins, liposomes, and nanoparticles. Bioconj. Chem. 16:1225-31, 2005.
(ELISA measurement of PEGylated compounds with AGP3 and E11)
Purchases can be made by contacting:
Ms. Mei Lee