Welcome to Anti-PEG Antibodies
PEG (polyethylene glycol) is a water-soluble, nontoxic, biocompatible polymer that has been approved by the Food and Drug Administration (FDA) for human intravenous, oral and dermal applications. Attachment of PEG chains to proteins can reduce their immunogenicity, minimize proteolytic cleavage and increase their serum half-life. PEG has also been attached to small molecules and liposomes for more selective delivery. PEG-modification of superparamagnetic iron oxide and quantum dots can improve their biocompatibility and reduce non-specific uptake.
To
allow sensitive measurement of PEGylated compounds, we developed
the first monoclonal antibody
(AGP3) with specificity for PEG. AGP3 can be employed to measure PEG-modified
protein by ELISA.
We also developed a monoclonal IgG antibody (E11) that
specifically binds to the backbone of PEG. Recently, we generated additional
high affinity anti-PEG monoclonal antibodies (AGP4 and
3.3-biotin). All our antibodies
can be employed to detect and quantify PEG-modified molecules in immunoblots, by ELISA and
by flow cytometry.
The Antibodies
AGP3 is our most utilized antibody for PEG detection. AGP3-biotin is also available, allowing convenient development of sandwich ELISAs.
E11 is an IgG1 monoclonal antibody that binds to PEG.
AGP4 is a new IgM antibody that binds to PEG with higher avidity than AGP3 and is an excellent capture antibody.
AGP4-biotin is now available as a sensitive detection reagent.
3.3 is an second generation IgG monoclonal antibody that binds to PEG with greater avidity than E11.
3.3-biotin is also avaible for use as a detection agent.
Of note, sandwich ELISA employing AGP4 to capture PEGylated compounds and 3.3-biotin for detection works very well, especially as compared to other commercially-available anti-PEG antibodies: Comparison with other antibodies
Samples of all our antibodies are available.
Commercial purchases can be made by contacting:
Ms. Mei Lee