Tumor Targeting and Prodrug Lab

Antibodies

We have generated monoclonal antibodies against several antigens and cell types. These antibodies are available for commercialization and for collaborative research projects.

Contact me at sroff@ibms.sinica.edu.tw for more information.

Antibody Index

Antigens

PEG Polyethylene glycol

More information and references about the anti-PEG antibodies can be found here ANTI-PEG

AFP Alpha fetoprotein (human)

PE Pseudomaonas exotoxin A

PLC delta 1 Phospholipase C delta 1 (human)

PLC delta 3 Phospholipase C delta 3 (human)

bG beta-glucuronidase (E. coli)

hbG beta-glucuronidase (human)

mbG beta-glucuronidase (murine)

RC RNAse RNAse (Bullfrog)

topo I Topoisomerase I (human)

Muc5 (human mucin)

TSG101 Tumor suppressor gene 101 (human)

MA HIV-1 matrix protein (p17(Gag))

TAL6 Tumor antigen TAL6 (human)

Cells

Caski Human cervical carcinoma

AS-30D Rat hepatoma

B16/F10 Mouse metastatic melanoma

B16/BL6 Mouse metastatic melanoma

Human cells Various human tumor and normal cells

CL1-0 Low metastatic human lung adenocarcinoma

CL1-5 High metastatic human lung adenocarcinoma

Virus

JEV Japanese encephalitis E protein

micoplasma

Human alpha-fetoprotein

Name	Antigen	Subclass	Comments
AFP3.3	human AFP	IgG2a	Binds to epitope 1 of AFP
1E8-E7-A11	human AFP	IgG1	Binds to epitope 1 of AFP
8B3-B11-E6	human AFP	IgG2b	Binds to epitope 1 of AFP
2C1-A10-A2	human AFP	IgG1	Binds to epitope 2 of AFP
4G8-B1-D10	human AFP	IgG1	Binds to epitope 2 of AFP
85-G4-C11	human AFP	IgG1	Binds to epitope 2 of AFP
1C10-B10-C10	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
3E9-F12-C10	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
4C10-H2-D6	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
6C10-A8-C1	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
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Pseudomonas exotoxin A

Name	Antigen	Subclass	Comments
2G6-11B	PE		not characterized
2B11-9G	PE		"
3A2-7C	PE		"
3E1-4B	PE		"
1D12-C3	PE		"
4G1-1F	PE		"
2B1-4G	PE		"
1C1-7C	PE		"
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Phospholipase C delta 1

Name	Antigen	Subclass	Comments
G	recombinant human PLC delta 1	IgG1
1C9	"	IgG1
3E8	"	IgG1
3H5	"	IgG1
5G5	"	IgG1
6A6	"	IgG1
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Phospholipase C delta 3

Name	Antigen	Subclass	Comments
3G1-B1	recombinant human PLC delta 3		binds dominant C-teminal domain epitope
8B6-B9	"		"
9E4-G11	"		"
9E5-B6	"	IgG1	"
10B11-B5	"	IgG1	"
2A11-D10-D8	N-terminal domain of PLC delta3		binds N-terminal domain
8G10-C12-B12	"		"
9H5-F5-B10	"		"
	Return to Index

E. coli beta-glucuronidase

Name	Antigen	Subclass	Comments
1E8	recombinant E. coli beta-glucuronidase	IgG1	good for western blots, ELISA
3C1	"	IgG1
6D1	"	IgG2a
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human beta-glucuronidase

Name	Antigen	Subclass	Comments
1B3-C1	rhuman beta-G	IgG1 lambda	ELISA +++, Western +++, no cross rxn mouse beta-G, does not inhibit activity
1B5-D3	rhuman beta-G	IgG2b kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
3F2-G10	rhuman beta-G	IgG1 kappa	ELISA +++, Western +++, no cross rxn mouse beta-G, does not inhibit activity
3G7-H12	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
5E1-H4	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
5G11-F9	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
7E1-A12	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
7F7-F3	rhuman beta-G	IgG2b lambda	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
7G8-B7	rhuman beta-G	IgG2b kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
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murine beta-glucuronidase

Name	Antigen	Subclass	Comments
7G7	rmurine beta-G	rat IgG	ELISA +++, FACs +++, Western -, no cross rxn human beta-G, does not inhibit activity
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RC RNAse

Name	Antigen	Subclass	Comments
RC1	Recombinant RNAse	IgG1	reacts with native RC bullfrog RNAse
RC2	"	IgG1	"
RC3	"	IgG2b	"
RC4	"	IgG1	"
RC5	"	IgG1	"
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Topisomerase I

Name	Antigen	Subclass	Comments
SR-TOP-1A	recombinant human topo I	IgM	ELISA +, Immunoblot +/-
SR-TOP-2A	"	IgM	ELISA +, Immunoblot +/-
SR-TOP-4A	"	IgG2b	ELISA +++, Immunoblot +++
SR-TOP-4C	"	IgG2b	ELISA +++, Immunoblot +++
4F2	"	ND	ELISA -, Immunoblot +
8D3	"	ND	ELISA ++, Immunoblot +
9F4	"	ND	ELISA -, Immunoblot +/-
	Return to Index

Muc 5

Name	Antigen	Subclass	Comments
5D5-G11	muc5	IgG1	reacts with human muc5 (mucin)
6E3-G10	"	IgG1	"
9B12-A2	"	IgG1
7D6-E6	"	IgG1
8A1-H2	"	IgG1
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Methoxy polyethylene glycol (PEG)

Name	Antigen	Subclass	Comments
AGP3	bG-sPEG conjugate	IgM	reacts with free and conjugated PEG in ELISA, immunoblots, in solution
E11	"	IgG1	"
AGP4		IgM	"
3.3		IgG1	"
	Return to Index		More info

Tumor Suppressor Gene 101 protein (tsg101)

Name	Antigen	Subclass	Comments
2A8-F11	recombinant human tgs101		binds to domain between aa 167-374, perinuclear and diffuse cytoplasmic immunofluorescence staining
3D8-H6	"	IgG1	binds to domain between aa 250-300, perinuclear and diffuse cytoplasmic immunofluorescence staining
3G1-D11	"		perinuclear immunofluorescence staining
6B3-G1	"
7H5-D6	"
10H12-C6	"	IgG1
	Return to Index

HIV matrix protein (p17)

Name	Antigen	Subclass	Comments
6D1-H3	recombinant MA		p17 (gag) / HIV matrix protein
9G8-C5	"
9G12-D4	"
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Tumor Associated Antigen L6

Name	Antigen	Subclass	Comments
1F4	TAL6 DNA immunization	IgG1	flow cytometer/ELISA
3C6	"	IgG1	"
6D6	"	IgG1	"
8D10	"	IgM	"
9C7	"	IgG1	"
10A9	"	IgG1	"
10H6	"	IgM	Western blots
2E7	"	IgM	dot blots / may recognize cryptic or internal epitope
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Human cervical carcinoma

Name	Antigen	Subclass	Comments
1H10	CaSki cells	IgG3	reacts with human cervical and colon carcinoma
9D5	SiHa cells		reacts with some human cervical carcinomas
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Rat hepatoma AS-30D cells

Name	Antigen	Subclass	Comments
RH1	AS-30D cells	IgG2a	reacts withAS-30D rat hepatoma cells
1C6-6C	"	IgG2a	"
6C6-4G	"	IgG2a	"
3F6-9B	"	IgG2a	"
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B16/F10 melanoma

Name	Antigen	Subclass	Comments
3D5	B16/F10 cells	IgM	reacts with B16/F10 melanoma cells (C57/BL6 mice)
E8	"	IgG2b	"
E9	"	IgG2b	"
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B16/BL6 melanoma

Name	Antigen	Subclass	Comments
1E9	B16/BL6 cells	IgM	reacts with B16/BL6 metastatic melanoma cells (C57/BL6 mice)
3D8	"	IgM	"
3F10	"	IgM	"
6G9	"	IgM	"
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Human cells

Name	Antigen	Subclass	Comments
AC-1	human cells	IgG1	reacts with most human cells
AC-2	"	IgG1	"
AC-3	"	IgM	mAbs available up to AC-10
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Low metastatic lung adenocarcinoma (CL1-0)

Name	Antigen	Subclass	Comments
6D8-B2	CL1-0 human lung adenocarcinoma cells		preferentially binds CL1-0 > CL1-5
8E6-C2	"		"
9F11-B1	"		"
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High metastatic lung adenocarcinoma (CL1-5)

Name	Antigen	Subclass	Comments
Ly-1F4	CL1-5 human lung adenocarcinoma cells	IgG1	binds CD13, partially inhibits aminopeptidase activity
Ly-1G9	"	IgG1	binds EphA2
Ly-2A2	"	IgG1	binds VLA-3 alpha (integrin alpha 3)
Ly-3C1	"	IgG1	binds EphA2
Ly-3E4	"	IgG1	preferentially binds CL1-5 > CL1-0
Ly-3F7	"	IgG2b	binds EphA2
Ly-4B8	"	IgG2b	binds CD13, goes not inhibit aminopeptidase activity
Ly-4F5	"	IgG1	preferentially binds CL1-5 > CL1-0
Ly-5D7	"	IgG1	"
Ly-6F10	"	IgG1	"
Ly-7F10	"	IgG1	"
Ly-8G6	"	IgG1	"
Ly-9A1	"	IgG1	binds CD13, partially inhibits aminopeptidase activity
Ly-9F7	"	IgG2b	preferentially binds CL1-5 > CL1-0
Ly-10D5	"	IgG1	"
Ly-10F3	"	IgG1	"
	Return to Index

Japanese encephalitis virus

Name	Antigen	Subclass	Comments
5G11-G3-B5	JEV	IgG2b	binds JEV-E protein
5E11-D9-G6	"	IgG2b	"
	Return to Index

Micoplasma

Name	Antigen	Subclass	Comments
2BS	infected cells	IgG3	detects micoplasma infected cells
4BF	"	IgG1	"
5BFII	"	IgG3	"
6BF	"	IgG2b	"
7BF3	"	IgG1	"
8BF	"	IgG2b	"
10BFII	"	IgM	"
11BFII	"	IgM	"
12BFII	"	IgM	"
17BFII	"	IgM	"
18BFII	"	IgM	"
	Return to Index

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