應用範例
Fig.1 GFP expression in mouse liver
Fig. 1 Comparison of liver transduction efficiency of different double-stranded AAV (dsAAV) serotypes.
ICR mice were intravenously injected with 1 x 10 12 vector genomes per mouse of dsAAV7/CB-GFP, dsAAV8/CB-GFP, or dsAAV9/CB-GFP vector. (a) Photographs of liver cryosections were taken 3 weeks after injection and examined for GFP expression. (b) Quantification of the average fluorescence intensity and the percentage of the total area containing GFP-positive cells. {Chen, C.C./Tao, M.H. (2009) Mol Ther 17: 352-9}
Fig.2 GFP expression in mouse brain
Fig. 2 AAV-GFP expression in mice neuron cell.
B6CBAFI/J mice were intrastriatally injected with single-stranded AAV8/CB-GFP. 5 wk after injection, brain sections were harvested and analyzed for GFP expression. {Ju, T.C./Chern, Y. (2011) J Cell Biol 194: 209-27}
Fig.3 GFP expression in mouse eye
Fig. 3 AAV-GFP expression in mice retina
B6CBAFI/J mice received subretinal injections using a 33-gauge needle and a Hamilton syringe. AAV administration was performed at 4 weeks of age, and the mice were euthanized at 10.5 weeks for GFP expression analysis. {Lin, M.S. et al. (2019) J Neurosci. 39: 1505-1524}
Fig.4 GFP expression in mouse heart
Fig. 4 AAV-GFP expression in mice heart.
1x10 12 vg of AAV9/CB-GFP were intraperitoneally injected into C57BL/6 mice. Heart cryo-sections were prepared 3 weeks after AAV administration, and images were acquired by confocal microscopy. {Sun, C.P. el al. (2021) PLoS Pathog 17: e1009758}
Fig.5 GFP expression in mouse lung
Fig.5 AAV-GFP expression in mice lung.
3x10 11 vg of AAV6/CB-GFP were intratracheally injected into C57BL/6 mice. 3 wk after injection, lung cryo-sections were harvested and analyzed for GFP expression. {Sun, C.P. et al. (2021) PLoS Pathog 17: e1009758}
Fig.6 GFP expression in mouse skeletal muscle
Fig.6 AAV-GFP expression in mice muscle.
1x10 11 vg of AAV8/CB-GFP were directly injected into quadriceps muscle of C57BL/6 mice . 3 wk after injection, muscle tissues were harvested and analyzed for GFP expression. (core facility’s own data)
Fig.7 GFP expression in mouse kidney
Fig.7 AAV-GFP expression in mice kidney.
1x10 11 vg of AAV8/CB-GFP were injected into C57BL/6 mice via sub-renal capsular injection. Kidneys were sectioned and analyzed for GFP expression 3 weeks post-injection. (core facility’s own data)
Fig.8 shRNA knockdown in mouse liver
Fig.8 Inhibition of HBV gene expression by AAV8 vectors in mice.
HBV transgenic mice were intrasplenically injected with 1 x 10 12 vg of various dsAAV8 vectors encoding HBV-specific HBV-S1 or the control GL2 shRNAs. At 14 days after injection, HBV core antigen in liver sections was analyzed by immunohistochemical staining. {Chen, C.C. et al. (2007) Gene Ther 14: 11-9}