應用範例

Fig.1 GFP expression in mouse liver

Fig. 1 Comparison of liver transduction efficiency of different double-stranded AAV (dsAAV) serotypes.

ICR mice were intravenously injected with 1 x 10 12 vector genomes per mouse of dsAAV7/CB-GFP, dsAAV8/CB-GFP, or dsAAV9/CB-GFP vector. (a) Photographs of liver cryosections were taken 3 weeks after injection and examined for GFP expression. (b) Quantification of the average fluorescence intensity and the percentage of the total area containing GFP-positive cells. {Chen, C.C./Tao, M.H. (2009) Mol Ther 17: 352-9}

Fig.2 GFP expression in mouse brain

Fig. 2 AAV-GFP expression in mice neuron cell.

B6CBAFI/J mice were intrastriatally injected with single-stranded AAV8/CB-GFP. 5 wk after injection, brain sections were harvested and analyzed for GFP expression. {Ju, T.C./Chern, Y. (2011) J Cell Biol 194: 209-27}

Fig.3

Fig. 3 AAV-GFP expression in mice retina

B6CBAFI/J were injected into the subretinal space of the eye using a 33 gauge needle with a Hamilton syringe. Mice injected with AAVs at the age of 4 weeks were killed at the age of 10.5 weeks and analyzed for GFP expression. {Lin, M.S./Chern, Y. (2019) J Neurosci. 39: 1505-1524}

Fig.4 GFP expression in mouse heart

Fig. 4 AAV-GFP expression in mice heart.

2 x 10 9 vector genomes of AAV8/CB-EGFP were directly injected into mouse cardiac muscle. 3 wk after the injection, heart sections were harvested and analyzed for GFP expression (provided by Dr. Chen C. C.).

Fig.5 shRNA knockdown in mouse liver

Fig. 5 Inhibition of HBV gene expression by dsAAV8 vectors in mice.

HBV transgenic mice were intrasplenically injected with 1 x 10 12 vector genomes of various dsAAV8 vectors encoding HBV-specific HBV-S1 or the control GL2 shRNAs. At 14 days after injection, HBV core antigen in liver sections was analyzed by immunohistochemical staining. {Chen, C.C./Tao, M.H. (2007) Gene Ther 14: 11-9}