應用範例

Fig.1 GFP expression in mouse liver

Fig. 1 Comparison of liver transduction efficiency of different double-stranded AAV (dsAAV) serotypes.

ICR mice were intravenously injected with 1 x 10 12 vector genomes per mouse of dsAAV7/CB-GFP, dsAAV8/CB-GFP, or dsAAV9/CB-GFP vector. (a) Photographs of liver cryosections were taken 3 weeks after injection and examined for GFP expression. (b) Quantification of the average fluorescence intensity and the percentage of the total area containing GFP-positive cells. {Chen, C.C./Tao, M.H. (2009) Mol Ther 17: 352-9}

Fig.2 GFP expression in mouse brain

Fig. 2 AAV-GFP expression in mice neuron cell.

B6CBAFI/J mice were intrastriatally injected with single-stranded AAV8/CB-GFP. 5 wk after injection, brain sections were harvested and analyzed for GFP expression. {Ju, T.C./Chern, Y. (2011) J Cell Biol 194: 209-27}

Fig.3 GFP expression in mouse eye

Fig. 3 AAV-GFP expression in mice retina

B6CBAFI/J mice received subretinal injections using a 33-gauge needle and a Hamilton syringe. AAV administration was performed at 4 weeks of age, and the mice were euthanized at 10.5 weeks for GFP expression analysis. {Lin, M.S. et al. (2019) J Neurosci. 39: 1505-1524}

Fig.4 GFP expression in mouse heart

Fig. 4 AAV-GFP expression in mice heart.

1x10 12 vg of AAV9/CB-GFP were intraperitoneally injected into C57BL/6 mice. Heart cryo-sections were prepared 3 weeks after AAV administration, and images were acquired by confocal microscopy. {Sun, C.P. el al. (2021) PLoS Pathog 17: e1009758}

Fig.5 GFP expression in mouse lung

Fig.5 AAV-GFP expression in mice lung.

3x10 11 vg of AAV6/CB-GFP were intratracheally injected into C57BL/6 mice. 3 wk after injection, lung cryo-sections were harvested and analyzed for GFP expression. {Sun, C.P. et al. (2021) PLoS Pathog 17: e1009758}

Fig.6 GFP expression in mouse skeletal muscle

Fig.6 AAV-GFP expression in mice muscle.

1x10 11 vg of AAV8/CB-GFP were directly injected into quadriceps muscle of C57BL/6 mice . 3 wk after injection, muscle tissues were harvested and analyzed for GFP expression. (core facility’s own data)

Fig.7 GFP expression in mouse kidney

Fig.7 AAV-GFP expression in mice kidney.

1x10 11 vg of AAV8/CB-GFP were injected into C57BL/6 mice via sub-renal capsular injection. Kidneys were sectioned and analyzed for GFP expression 3 weeks post-injection. (core facility’s own data)

Fig.8 shRNA knockdown in mouse liver

Fig.8 Inhibition of HBV gene expression by AAV8 vectors in mice.

HBV transgenic mice were intrasplenically injected with 1 x 10 12 vg of various dsAAV8 vectors encoding HBV-specific HBV-S1 or the control GL2 shRNAs. At 14 days after injection, HBV core antigen in liver sections was analyzed by immunohistochemical staining. {Chen, C.C. et al. (2007) Gene Ther 14: 11-9}

代表性文章

  1. Y. T. Tung et al., Mir-17 approximately 92 Confers Motor Neuron Subtype Differential Resistance to ALS-Associated Degeneration. Cell stem cell 25, 193-209 e197 (2019).
  2. C. P. Sun et al., Rapid generation of mouse model for emerging infectious disease with the case of severe COVID-19. PLoS Pathog 17, e1009758 (2021).
  3. Y. T. Weng et al., TRAX Provides Neuroprotection for Huntington's Disease Via Modulating a Novel Subset of MicroRNAs. Mov Disord 37, 2008-2020 (2022).
  4. S. C. Wu et al., Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth. Nature communications 13, 4174 (2022).
  5. C. Y. Kao et al., Improvement of Gene Delivery by Minimal Bacteriophage Particles. ACS Nano 17, 14532-14544 (2023).
  6. I. J. Lee et al., A receptor-binding domain-based nanoparticle vaccine elicits durable neutralizing antibody responses against SARS-CoV-2 and variants of concern. Emerg Microbes Infect 12, 2149353 (2023).
  7. C. P. Sun et al., Development of AAV-delivered broadly neutralizing anti-human ACE2 antibodies against SARS-CoV-2 variants. Mol Ther 31, 3322-3336 (2023).
  8. P. S. Sung, C. P. Sun, M. H. Tao, S. L. Hsieh, Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc. EMBO Mol Med 15, e16351 (2023).