應用基因轉殖 (transgenic) 及基因剔除 (knockout) 小鼠來探討帶功能性多醣體 (functional glycans) 的細胞表面蛋白之分子、生化特性及病生理功能﹐著重於心血管疾病及癌症生物學之探討。

具分子生物、生化或生命科學相關科系之碩士/博士學位，

對研究工作有濃厚興趣、能自我挑戰者。

協助精準醫療相關計畫

1.具生醫、醫護等相關科系背景

2.具醫院臨床工作經驗尤佳

OVERVIEW

The Cheng lab  (https://thechenglab.com/) is looking for an enthusiastic and independent Postdoctoral Fellow to join our dynamic and competitive group in human immunology research in the Institute of Biomedical Science at Academia Sinica, Taiwan. Our research focuses on deep-profiling human virus-specific T cells during viral infection, vaccination, or virus-associated cancer by using highly multiplexed combinatorial peptide-MHC tetramer staining, mass cytometry, single-cell sequencing methods (Cheng et al., 2019. Science Immunology; Cheng et al., 2021. Immunity), and high-dimensional analysis (Newell and Cheng., 2016. Nature Immunology). We are interested in dissecting the different arms of human adaptive immune responses, and characterize the functional and cellular heterogeneity of tissue-resident memory T cells or exhausted T cells. We are currently working on chronic hepatitis B infection (HBV), HBV-associated hepatocellular carcinoma and liver disease, SARS-CoV-2 infection and vaccination, human papillomavirus (HPV)-associated cancers.

RESPONSIBILITIES

1. Perform mass cytometry experiment, maintenance, and the preparation of reagents and materials.
2. Use highly multiplexed combinatorial peptide-MHC tetramer staining and single-cell sequencing methods to deep-profile human antigen-specific T cells. Establish Smart-seq3xpress protocol.
3. Perform high-dimensional data analysis and data interpretation.
4. Independently carry out high quality research, support other lab members’ projects, write manuscripts, and present findings in international conferences.
5. Train and mentor junior members in the lab. Identify, communicate, and solve scientific problems.

QUALIFICATIONS

Minimum qualifications:

• Proactive and passionate in biomedical research and immunology
• Holds MD, PhD, MD/PhD, or equivalent degree in related fields
• Strong experience in flow cytometry, and other experimental tools in the research fields of immunology and molecular and cellular biology
• Experience in cell and tissue culture, and ex vivo and in vitro experiment
• Great organization and communication (verbal and writing) skills
• Team player with great interpersonal characteristics

Preferred qualifications (candidates who have the following criteria will be considered as favored):

• Have done research in chronic viral infection (e.g., LCMV, HBV, HCV, and HIV), or have research background in virology or viral immunology
• Experience in handling and processing human clinical samples and primary tissue cells
• Experience in tissue sectioning, imaging and histological staining
• Experience in TCR sequencing and analysis
• Familiar in R or python

OVERVIEW

The Cheng lab (https://thechenglab.com/) is looking for an enthusiastic and excellent Research Technician to join our dynamic and competitive group in human immunology research in the Institute of Biomedical Science at Academia Sinica, Taiwan. Our research focuses on deep-profiling human virus-specific T cells during viral infection, vaccination, or virus-associated cancer by using highly multiplexed combinatorial peptide-MHC tetramer staining, mass cytometry, and single-cell sequencing methods (Cheng et al., 2019. Science Immunology; Cheng et al., 2021. Immunity), and high-dimensional analysis (Newell and Cheng., 2016. Nature Immunology). We are interested in dissecting the different arms of human adaptive immune responses, and characterize their functional and cellular heterogeneity, particularly tissue-resident memory T cells and follicular helper T cells. We are currently working on chronic hepatitis B infection (HBV), HBV-associated hepatocellular carcinoma and liver disease, and SARS-CoV-2 infection and vaccination.

RESPONSIBILITIES

1. Learn nuances of mass cytometry experiment, maintenance, and the preparation of reagents.
2. Produce proteins required for highly multiplexed combinatorial peptide-MHC tetramer staining.
3. Use single-cell sequencing methods to further profile human antigen-specific T cells.
4. Perform high-dimensional data analysis.
5. Carry out high quality research under the supervision of Dr. Yang Cheng or senior member in the lab, and support other lab members’ projects.
6. Lab management.

QUALIFICATIONS

Minimum qualifications:

• Passionate in science
• Bachelor’s degree in related scientific fields
• Cell and tissue culture, experience in common techniques in molecular and cellular biology
• Great organization and communication (verbal and writing) skills
• Team player with great interpersonal characteristics
• Experience in flow cytometry

Preferred qualifications (candidates who have the following criteria will be considered as favored):

• Experience in handling and processing human clinical samples
• Certified phlebotomist
• Experience in molecular cloning, and protein production and purification
• Experience in TCR sequencing
• Experience in FACS sorting
• Experience in spectral flow cytometry
• Experience in R (or python)

-魚房管理、清潔與動物照顧 (淡水小型魚)

-實驗室器材清潔、滅菌、環境整理與其他庶務

-個性認真負責、積極任事、團隊合作

-早班八點開始,排班時間彈性可議

-需假日輪班,能接受才應徵 (動物餵食1-2小時)

具分子生物、生化、或動物實驗研究背景者

碩/博士畢業五年內對細胞訊息傳遞、癌症研究有興趣者

細胞免疫相關機制探討

有小鼠實驗經驗佳

具分子細胞生物學背景

DC-T 細胞免疫研究，探討細胞免疫機轉 (長期計畫支持)

疫苗開發研究

動物模型操作

具分子細胞生物學背景

Developing anticancer drugs and studies on molecular mechanisms of tumor metastasis

Experience on drug development and skills of molecular, cellular and genomic biology
 

1.實驗動物獸醫學照護與諮詢

2.動物設施例行巡房、計畫核准後監督(PAM)

3.訂定動物檢疫策略計畫（排定行程委託國動代檢）

4.動物房環境監測(落塵、落菌、水質、滅菌確校)

5.分生實驗，動物實驗及儀器、實驗室藥品管理

6.支援動物飼育與照護、協助User解決操作問題

7.其他行政事務及配合IACUC委員會之定期查核監督

8.舉辦教育訓練及修訂動物房操作SOP

需具備獸醫師資格，有動物實驗、無菌操作經驗者佳