Service
- Immunohistochemistry (IHC)

Introduction

  • Immunohistochemistry (IHC) staining is performed on formalin fixed paraffin embedded tissue sections.
  • ICF provide antibodies for IHC staining of immune cells, including T cell (CD3, CD4, CD8), B cell (B220), macrophage (F4/80), neutrophil (Ly-6G).
  • Customization services can be performed with clients provided antibodies.
Cell type Cell markers Species Organ
T cell CD3 Mouse Spleen, Thymus*
CD4 Spleen*
CD8 Spleen*
B cell B220 Spleen, Lymph node*, Thymus*
Macrophage F4/80 Colon
Neutrophil Ly-6G Colon
*fixed with Zinc fixative buffer
Examples:
IHC example

Process

process

Sample Preparation

For CD4 and CD8, mouse tissue should be fixed with Zinc fixative buffer followed by paraffin embedding.


Price

Item Research institute (NTD/slide) Company (NTD/slide)
IHC (use client's antibody) 600 1000
IHC (use facility’s antibody) 650 1050

Payment Procedure

billing procedures pic

Payment Procedure

  1. Users fill in application form.
  2. After the application forms are approved, the facility will send a remittance advice email to each applicant.
  3. Arrange the payment.
  4. After the payment has arrived, the receipt and application form will be sent to users
  5. Upon receiving the sample delivery notification and the receipt, you may send the samples to the facility during working hours.

※A partial payment is also accepted if the total payment exceeds your quota. (For example, if the estimated price exceeds 300,000 NTD, two payments of 50% each can be made on the current and following month)

[Notice for payment]
  1. The charge is non-refundable, so please confirm the service charge before payment. After the payment has arrived, we will start to execute the service.
  2. If you have some special reasons for not delivering the payment before the service , please fill the “Deferred Payment Affidavit Letter”( Download here ) and send it to the core. The core manager will confirm this letter and execute this service before the payment. Each case can only apply once.
  3. Due to the different purchasing process of each administrative unit, it may takes 3-4 weeks from user submitting the purchase request to the unit to they actually allocate the funds to the Academia Sinica account. Please take the time requirement into consideration during scheduling the experiment.
[Notice for sample delivery]
  1. After the core ahs received the payment, we will send a “Sample Delivery Notification” to user. Please deliver your sample within one month. If we don’t receive the sample within a month, we will send the sample delivery notification again. However, if the sample is not delivered for more than three months, the case will be regarded as abandonment and no re-fund will be given.
  2. If there are some special circumstances that the user need to extend the delivery deadline, please inform the core and tell us the expected sample delivery date. If the expected delivery date is not clearly informed, the delivery deadline will be automatically extended by one month. Each case can only apply once.

Payment method for users in Academia Sinica

  • Please reply the email to agree the fund transfer.
  • The facility will transfer the payment through the in-house PI payment system (quota).
  • If users in Academia Sinica use Ministry of Science and Technology (MOST) funds to pay for the use of facilities, the charge calculation will be based on the "Research institute outside Academia Sinica" fee standard

Payment methods for users in other academia units or company

Please choose one payment method from below.

  1. Wire transfer
    • Beneficiary account number: 004056030013
    • Beneficiary account name: 中央研究院301專戶
    • Beneficiary bank and branch: LAND BANK OF TAIWAN NAN KANG BRANCH.
    • Please provide the information below via Email/fax after remit the money.
      1. Name of core facility service you use
      2. Client’s Title
      3. Client’s Name
      4. Client’s Address
      5. Amount of receipts (one receipt for each case/or one receipt for multiple cases)
      6. Confirmation of the payment (wire transfer/ cash remittance note)
      7. Service application form
    • Receipt will be sent within one week
  2. Send a check by registered mail
    • Check title: Academia Sinica (中央研究院)
    • Mail address: Room 2103, Academic Affairs and Instrument Service, Academia Sinica., No. 128, Sec. 2, Academia Rd, Nankang Dist., Taipei City 11529, Taiwan (ROC)
    • Recipient: Mr. Wun-Ci Ciou (邱文棋)
    • Receipt will be sent within one week
  3. Direct cash/ check deposit
    • Please bring cash or check to Room 2103, Administrative Building, Instrument Management Department, Academic Affairs and Instrument Service, Academia Sinica and look for Mr. Wun-Ci Ciou (邱文棋)
    • The receipt will be given to you at once

Order

Please submit your application form to: inflamcore@ibms.sinica.edu.tw

Download form

IHC
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