Service - Analysis

Introduction

The major activity of this core is to provide multi-plex assay to measure mediator levels in patients’ sera.

Multiplex assay is a technology that allows for the simultaneous quantification of analytes in serum/plasma and culture supernatants, making them a useful tool in both disease mechanistic studies and in biomarker discovery. Available analytes include cytokines, chemokines and growth factors. We currently offer both fully validated cytokine/chemokine panels and subpanels and other panels pertaining to immunology, metabolism, toxicity, etc. Multiplex assay panels are customizable to the investigators’ needs. For projects, prior consultation with the laboratory is recommended to ensure optimal sample processing.

The multiplex is capable of measuring up to 100 analytes. Multiplex assay can be used for the detection of multiple cytokines in serum, plasma or supernatants. It‘s consist of beads dyed with differing concentrations of two fluorophores to generate distinct bead sets. Each bead set is coated with a capture antibody specific for a specific cytokine. A second biotinylated antibody and streptavidin-phycoerythrin (S-PE) is used to detect the captured cytokine. The Bio-plex 200 analyzer is a dual laser, flow-based, sorting and detection platform. One laser detects the beads and determines which cytokine is being detected. The second laser determines the magnitude of PE-derived signal, which is proportional to the amount of cytokine bound.


Process

process

Sample Preparation

Serum prep

  • Allow blood to clot at room temperature for 30-45 min before proceeding to centrifugation steps.
  • Centrifuge at 1,000 x g for 15 min at 4C and transfer into a clean tube.
  • Repeat centrifuge at 1,000 x g for 10 min at 4C to completely remove platelets and precipitates.
  • Aliquot and store undiluted sample at -80 ℃ if not using immediately.

Plasma prep

  • Use collection tubes coated with anticoagulant (EDTA is the recommended anticoagulant for preparing plasma samples)
  • Centrifuge at 1,000 x g for 15 min at 4C and transfer into a clean tube.
  • Repeat centrifuge at 1,000 x g for 10 min at 4C to completely remove platelets and precipitates.
  • Aliquot and store undiluted sample at -80℃ if not using immediately.

Note

  • Use protease inhibitor when profiling for diabetes/metabolic markers.
  • Extremely lipemic samples may be filtered with a 0.22 µm filter to prevent clogging.
  • Hemolyzed samples are not suitable for Bio-Plex Pro™ diabetes assays.
  • Serum is qualitatively different from plasma because of how the removal of fibrinogen and other proteins that form fibrin clots affects coagulation.
  • Protein concentration of serum is 3~4% less than that of plasma.
  • Extremely lipemic samples may be filtered with a 0.22 µm filter to prevent clogging.

Price

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Research institute outside
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Company
1 25,000 28,000 38,000
2 50,000 56,000 76,000
3 75,000 84,000 114,000
4 100,000 112,000 152,000
5 125,000 140,000 190,000
6 145,000 163,000 223,000
7 170,000 191,000 261,000
8 195,000 219,000 299,000
9 220,000 247,000 337,000
10 245,000 275,000 375,000
11 265,000 298,000 408,000
12 290,000 326,000 446,000
13 315,000 354,000 484,000
14 340,000 382,000 522,000
15 365,000 410,000 560,000
16 385,000 433,000 593,000
17 410,000 461,000 631,000
18 435,000 489,000 669,000
19 460,000 517,000 707,000
20 485,000 545,000 745,000

Billing Procedures

billing procedures pic

繳費流程

  1. 使用者填單申請
  2. 核心實驗室審核無誤後,再由核心E-mail通知使用者進行繳款。
  3. 使用者繳款。(如有中研院院內經費,建議先行使用院內繳費方式)
  4. 繳款確認後,即可寄送樣本並進行服務。
  5. 收款通知,收款完成後將提供使用紀錄予申請者以利存檔備份。

※若超過額度,可先繳交部分之費用,即可進行服務。(例如:若sample數目超過240且cytokine超過12個,總價超過30萬可先預繳50%之費用並進行服務)

院內繳費方式

  • 請申請者回覆信件同意扣款。
  • 核心將經由院內PI轉帳系統執行轉帳。

院外繳費方式

請使用者選擇一種繳費方式完成付款。

  1. 電匯
    • 帳號:004056030013
    • 戶名:中央研究院301專戶
    • 銀行:土地銀行南港分行
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    • 郵寄地址:115 台北市南港區研究院路二段128號
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Order

Please submit your application form to: inflamcore@ibms.sinica.edu.tw

Download form

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We will help to performance cell types stain on slide.

Immunohistochemistry (IHC)

The Immunohistochemistry test example.

ihc pic

CD3、CD4、CD8:T Cell, B220:B Cell, F4/80:Macrophage, Ly-6G6C:Neutrophil

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