M.D., Taipei Medical University Postdoctoral Fellow, University of California, San Diego
With the growth of genetic engineering, mice have become common models of human cardiovascular disease and therefore have stimulated the development of techniques to monitor and image the murine cardiovascular system. We had established techniques with cardiac trans-aortic banding, coronary artery ligation, cardiac ischemic reperfusion, and has established the following in vivo analysis in the past several years including surface ECG, echocardiography, wireless radio-telemetry and heart rate variability studies. Through collaboration with colleagues in our Institute, we have worked on several models of cardiovascular diseases (ASIC3, ATF3). In the past two years, we also established reactive oxidative stress (ROS) related animal model on cardiovascular diseases. Here we used carboplatin or gentamicin induced ROS on heart, liver, and kidney respectively, then to elucidate the therapeutic effects using stains (pravastatin, simvastatin) or adiponectin treatment. We specifically explored the therapeutic mechanism related to anti-apoptosis and anti-inflammation, and the possible involvements on HO-1 and PPARα signaling pathways in such diseases. Recently, we are working on the novel molecular mechanism involved in iron overload cardiomyopathy, and elaborate the effect of using adiponectin supplement or thromboxane A2 inhibition for possible therapeutic molecules in the future.