Dr. Yang, Kai-Chien 's publons link picture

Dr. Yang, Kai-Chien

Joint Appointment Associate Research Fellow
  • 2652-3597 (Lab) (Room No: N717)

  • Organ fibrosis and stromal biology
  • Cardiac regeneration
  • Non-coding RNA biology
  • Ion channel regulation and electrophysiology
  • Cardiac oxidative stress and arrhythmias

Education and Positions:
    • M.D. National Taiwan University
    • Ph.D. Washington University in St. Louis
    • Associate Professor, Department and Graduate Institute of Pharmacology, National Taiwan University
    • Attending physician, Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital

Highlight Detail

Deep RNA Sequencing Reveals Dynamic Regulation of Myocardial Noncoding RNAs in Failing Human Heart and Remodeling With Mechanical Circulatory Support

Dr. Yang, Kai-Chien
Circulation, Mar 04, 2014

Clinical management of heart failure requires reliable and accurate tools to categorize patients on the basis of underlying causes, associated risks, treatment responses, and outcomes. In recent years, myocardial transcriptional profiling has been used extensively to this end. Most prior transcriptional profiling studies in heart failure, however, examined only cardiac mRNAs or microRNAs (miRNAs), which together constitute only a small portion (≈1%) of the human genome. Because a large proportion of the genome is transcribed as long noncoding RNA (lncRNA), we profiled lncRNAs in parallel with mRNAs and miRNAs to provide a complete picture of the human cardiac transcriptome landscape in heart failure. Exploiting next-generation sequencing technology, we conducted a comprehensive cardiac transcriptome profiling study encompassing mRNAs, miRNAs, and lncRNAs in human left ventricular samples from heart failure patients before and after mechanical circulatory support with a left ventricular assist device and in left ventricular samples from nonfailing donors. We demonstrate here that the expression signature of cardiac lncRNAs compared with those of mRNAs or miRNAs provides exquisite sensitivity to discriminate cardiomyopathic samples of ischemic and nonischemic origins and to distinguish heart failure samples before and after left ventricular assist device support, revealing the potential of using lncRNA profiles as a reliable biomarker to differentiate between cardiac disease states. We further provide evidence to show that cardiac lncRNAs likely function through regulating the expression of nearby (cis) rather than distant (trans) genes. Taken together, these studies suggest an important role for lncRNAs in the pathogenesis of heart failure and in reverse remodeling observed after ventricular unloading with mechanical support.