Dr. Liao, You-Di 's publons link picture

Dr. Liao, You-Di

Adjunct Research Fellow
  • 2652-3053 (Office)
Specialty:
  • Protein Chemistry
  • Antitumor Ribonucleases
  • Antimicrobial Protein/Peptides
Education and Positions:

  • Ph.D. National Taiwan University

 1. Ribonucleases with antitumor activity are mainly found in the oocytes and embryos of Rana catesbeiana (bullfrog), but the mechanism of antitumor activity and their roles in frog development are not clear. The residues involved in catalytic activity base recognition and cytotoxicity are investigated by site-directed mutagenesis, NMR and X-ray cocrystallography.

 

2. Antimicrobial peptides/proteins (AMPs) are important components ofthe host innate defense mechanisms against invading microorganisms.The human ribonuclease 7(hRNase 7) possesses membrane permeability and antimicrobial activity through the flexible and clustered lysine residues near N-terminus. In addition to hRNase 7, hundreds of AMPs also exert similar antimicrobial activity. The susceptibility of Gram negative bacteria to these AMPs is determined by an outer membrane protein, like the OprI of Pseudomonas aeruginosa and Lpp of E. coli. Therefore, OprI and its homologues may be used as ligands for the screening and production of antimicrobial materials.

 

3. The removal of N-terminal Met is often crucial for the function and stability of recombinant proteins. We engineer an E. coli methionine aminopeptidase (MetAP) which is able to remove N-terminal Met from bulky or acidic penultimate residues.

Our Team
Team photo

Journal 40 Book 0

  1. Tang WH, Wang SH, Wang CF, Mou Y, Lin MG, Hsiao CD, Liao YD The lipid components of high-density lipoproteins (HDL) are essential for the binding and transportation of antimicrobial peptides in human serum. Scientific reports 12(1), 2576 (2022) [JCR] [WOS]
  2. Jung CJ, Liao YD, Hsu CC, Huang TY, Chuang YC, Chen JW, Kuo YM, Chia JS Identification of potential therapeutic antimicrobial peptides against Acinetobacter baumannii in a mouse model of pneumonia. Scientific reports 11(1), 7318 (2021) [JCR] [WOS]
  3. Tang Wen-Hung, Wang Chiu-Feng, Liao You-Di Fetal bovine serum albumin inhibits antimicrobial peptide activity and binds drug only in complex with α1-antitrypsin Scientific Reports 11(1) ,1267 (2021) [JCR] [WOS]
  4. Wang Shih-Han, Tang Tony Wen-Hung, Wu Eden, Wang Dan-Wei, Liao You-Di Anionic Surfactant-Facilitated Coating of Antimicrobial Peptide and Antibiotic Reduces Biomaterial-Associated Infection ACS Biomaterials Science & Engineering 6(8), 4561-4572 (2020) [JCR] [WOS]
  5. Wang Shih-Han, Tang Tony Wen-Hung, Wu Eden, Wang Dan-Wei, Wang Chiu-Feng, Liao You-Di Inhibition of bacterial adherence to biomaterials by coating antimicrobial peptides with anionic surfactant Colloids and Surfaces B: Biointerfaces 196, 111364 (2020) [JCR] [WOS]
  6. Wang SH, Wang CF, Chang TW, Wang YJ, Liao YD Oligomerization and insertion of antimicrobial peptide TP4 on bacterial membrane and membrane-mimicking surfactant sarkosyl. PloS one 14(5), e0216946 (2019-05) [JCR] [WOS]
  7. Chang TW, Wei SY, Wang SH, Wei HM, Wang YJ, Wang CF, Chen C, Liao YD Hydrophobic residues are critical for the helix-forming, hemolytic and bactericidal activities of amphipathic antimicrobial peptide TP4. PloS one 12(10), e0186442 (2017) [JCR] [WOS]
  8. Wei HM, Lin LC, Wang CF, Lee YJ, Chen YT, Liao YD Antimicrobial Properties of an Immunomodulator - 15 kDa Human Granulysin. PloS one 11(6), e0156321 (2016) [JCR] [WOS]
  9. Tseng TS, Wang SH, Chang TW, Wei HM, Wang YJ, Tsai KC, Liao YD, Chen C Sarkosyl-Induced Helical Structure of an Antimicrobial Peptide GW-Q6 Plays an Essential Role in the Binding of Surface Receptor OprI in Pseudomonas aeruginosa. PloS one 11(10), e0164597 (2016) [JCR] [WOS]
  10. Lin CH, Chen JK, Ko TM, Wei CY, Wu JY, Chung WH, Chen SY, Liao YD, Hung SI, Chen YT Immunologic basis for allopurinol-induced severe cutaneous adverse reactions: HLA-B*58:01-restricted activation of drug-specific T cells and molecular interaction. The Journal of allergy and clinical immunology 135(4), 1063-1065.e5 (2015) [JCR] [WOS]
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