Dr. Lee, Yungling Leo李永凌 博士

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Dr. Lee, Yungling Leo
李永凌 博士

Associate Research Fellow
長聘副研究員



  • M.D. National Taiwan University

    Ph.D. National Cheng Kung University

  • 02-27899132 (Lab) (Room No: N343)
  • 02-26523013 (Office)
  • 02-27829142 (Fax)
Specialty:

1. Adaptive Immunity

2. Omics and Precision Medicine

3. Antigen Presenting Cells

4. Vaccine Development

RESEARCH

Clinical Immunology Lab: linking innate cells to adaptive immunity

 

T cell Regulation in Inflammatory Diseases

1. We have demonstrated that UVB-exposed Treg cells contributed to the alleviation of inflammatory skin in a murine model of OVA-induced skin inflammation under UVB irradiation. When depleting Treg cells in Foxp3DTR/GFP mice by the administration of diphtheria toxin, we found UVB irradiation could not suppress OVA-induced skin inflammation. In addition, we found that LAG-3 expression was significantly increased in UVB-exposed Treg cells.

 

2. Cytokine signaling between keratinocytes and innate cells results in CD4/CD8/Treg cell infiltration of psoriasis lesions. We applied single-cell mass cytometry (Cytometry Time-Of-Flight, CyTOF) to investigate function of Treg cells in the peripheral blood of patients with psoriasis. We will also apply single-cell RNA sequencing (scRNA-seq) to dissect the differential pattern among skin cells of patients before and after UVB therapy.

 

DC-based Cancer Immunotherapy

1. Emerging evidence suggests the promoting role of aromatic hydrocarbon receptor (AhR) in the initiation, promotion, progression, invasion, and metastasis of cancer cells. AhR-CD11c-conditional KO mice were generated and we found tumor growth were significantly reduced in conditional KO mice. It was also noted that activation of AhR can induce PD-L1 level on dendritic cell (DC) and Treg recruitment.

 

2. We employed phage display to select anti-CD11c peptide and AhR-neutralizing single-chain fragment variable (ScFv). PEGylated peptide is inserted into surface of liposome and the transcript of neutralizing ScFv is packaged in the inner space of liposome. Liposome coats acid-labile PEG-PGA-HX to prevent systemic peptidase and accelerating endosomal escape in acidic tumor microenvironment. Finally, we implemented the humanized ASID mice to investigate the efficiency of liposomal drugs. The DC-targeted cancer treatment remodels the tumor microenvironment and reduces side effects.

 

 

HIGHLIGHT 重要成果

Investigating Obesity-related Risk Factors for Childhood Asthma
Pediatric Allergy and Immunology, Dec 02, 2021

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