Dr. Chen, Chinpan
Emeritus Research Fellow
- 02-26523035 (Lab) (Room No: N133)
- 02-27887641 (Fax)
-
Specialty:
- Structural Biology
- NMR
Education and Positions:
Ph.D. University of Washington
Highlight Detail
Protein–DNA complex-guided discovery of the antibacterial lead E1 for restoring the susceptibility of Klebsiella Pneumoniae to polymyxin B by targeting the response regulator PmrA
Dr. Chen, Chinpan
Chemical Communications, May 24, 2018A new antibacterial drug is urgently needed. We employed a protein–DNA complex-guided pharmacophore modeling approach to screen inhibitors against the response regulator PmrA of polymyxin B-resistant Klebsiella pneumoniae (KP). The identified lead, E1 (IC50 = 10.2 μM), targeted the DNA-binding domain of PmrA (KD = 1.7 μM), whose conserved residues R171, R198, K203, and Y214 have been shown to be hotspots for antimicrobial development. Treatment of E1 restored the susceptibility of KP to polymyxin B.