Dr. Lee, Yu-Ru
Assistant Research Fellow
- 2789-9137 (Lab) (Room No: R401)
- 2652-3931 (Office)
- yrlee@ibms.sinica.edu.tw
Specialty:
- Cancer Genetics
- Cancer Signaling, Post-translational Modifications, Non-coding RNAs
- Cancer Biology, Targeted/Immunotherapy
Education and Positions:
- Ph.D., Institute of Molecular Medicine, National Taiwan University College of Medicine
- Instructor in BIDMC, Harvard Medical School
- Post-doc in BIDMC, Harvard Medical School
Highlight Detail
Dual DNA and protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia
Dr. Lee, Yu-Ru
Nature Methods, Mar 18, 2021
The architecture of chromatin regulates eukaryotic cell states by controlling transcription factor access to sites of gene regulation. Here we describe a dual transposase-peroxidase approach, integrative DNA and protein tagging (iDAPT), which detects both DNA (iDAPT-seq) and protein (iDAPT-MS) associated with accessible regions of chromatin. In addition to direct identification of bound transcription factors, iDAPT enables the inference of their gene regulatory networks, protein interactors and regulation of chromatin accessibility. We applied iDAPT to profile the epigenomic consequences of granulocytic differentiation of acute promyelocytic leukemia, yielding previously undescribed mechanistic insights. Our findings demonstrate the power of iDAPT as a platform for studying the dynamic epigenomic landscapes and their transcription factor components associated with biological phenomena and disease.