人類細胞株鑑定之樣品準備說明
Sample Submission Guidelines for human cell line authentication
1. 樣品類型
接受細胞樣品及DNA樣品作為鑑定材料,所有樣品需裝於1.5 mL 離心管中遞交。本服務僅接受人類細胞株。
- 1.1 細胞樣品
- 細胞數量:每份樣品需提供1x10⁶ ~ 5x10⁶ 細胞。
- 樣品準備方式:
- 貼附型細胞:
- 使用Trypsin剝離細胞,計數後取適量細胞。
- 以1500 rpm 離心 5 分鐘,形成細胞沉澱並去除上清液。
- 使用PBS 緩衝液清洗細胞,再次離心後移除上清液,僅留細胞沉澱送驗。
- 懸浮型細胞:
- 收集細胞並計數,取適量細胞。
- 使用PBS 緩衝液清洗細胞,離心後移除上清液,僅留細胞沉澱送驗。
- 1.2 DNA樣品
- 濃度要求:最低濃度為10 ng/µL,建議濃度為50~100 ng/µL以達最佳效果。
- 體積要求:無論濃度,樣品體積至少需10 µL。
2. 注意事項
- 冷凍保存的細胞相比於新鮮培養細胞,可能導致結果品質較低。
- 若為病毒細胞株,需提前提取 DNA再進行送樣。病毒細胞沉澱物不予受理。
- 本服務僅接受人類細胞株。
1. Sample Types
We accept cell samples and DNA samples for authentication. All samples must be submitted in a 1.5 mL microfuge tube. Only human cell lines are accepted.
- 1.1 Cell Samples
- Quantity: Provide 1x10⁶ ~ 5x10⁶ cells per sample.
- Preparation:
- Adherent Cells:
- Detach cells using Trypsin and count them.
- Centrifuge at 1500 rpm for 5 minutes to pellet the cells, then remove the supernatant.
- Wash cells with PBS, centrifuge again, and remove the supernatant. Submit the cell pellet.
- Suspension Cells:
- Collect and count the cells.
- Wash cells with PBS, centrifuge, and remove the supernatant. Submit the cell pellet.
- 1.2 DNA Samples
- Concentration: Minimum 10 ng/µL; recommended 50~100 ng/µL for optimal results.
- Volume: Submit at least 10 µL, regardless of concentration.
Notes
- Cryopreserved cells may yield lower-quality results compared to freshly cultured cells.
- For virus cell lines, DNA must be extracted prior to submission. Virus cell pellets are not accepted.
- Only human cell lines are accepted for this service.