Flow Cytometry流式細胞儀設施

Brand

EQUIPMENT

 

 


Flow analyzers

 

  • LSRII SORP – 17 color analyzer

 

 

 

 

 

 

 

 

 

Brand:Becton Dickinson

Available Excitation wavelength(nm)355, 404, 488, 561, 639

Available fluorescense detectors:17

Flow rate(μL/min)12, 24, 36, 60, 72, 120

Analysis speed:70,000 events/sec

Sample input:5mL Falcon Polystyrene tube (ref. 352052, 352054, 352235), 96 well plate, 384 well plate

Software:BD FACSDiva software v6.2


  • Attune CytPix – 14 color image flow analyzer

 

 

 

 

 

 

 

 

 

BrandThermo Fisher Scientific

Available Excitation wavelength(nm)405, 488, 561, 637

Available fluorescense detectors14

Flow rate(μL/min)12, 25, 100, 200, 500, 1000

Analysis speed35,000 events/sec

Sample inputeppendorff, micro tube, 5mL tube

Autosampler input: eppendorff, 96- & 384-well plate (standard & deep well)

Image type: bright-field images (no fluorescent images)

Magnification: 20X objective (0.45 NA)

Image resolution: 0.3um/pixel

Image sensitivity: > 800nm particles

Image capture rate: ~ 6000 images/sec

Image size: 96x96 (pixel) ~ 248x248 (pixel)

SoftwareAttune NxT software v5.2


  • Attune NxT – 14 color analyzer

 

 

 

 

 

 

 

 

 

 

 

 

 

BrandThermo Fisher Scientific

Available Excitation wavelength(nm)405, 488, 561, 637

Available fluorescense detectors14

Flow rate(μL/min)12, 25, 100, 200, 500, 1000

Analysis speed35,000 events/sec

Sample inputeppendorff, micro tube, 5mL tube

SoftwareAttune NxT software v4.2


  • FACSCanto – 6 color analyzer

 

 

 

 

 

 

 

 

BrandBecton Dickinson

Available Excitation wavelength(nm)488, 633

Available fluorescense detectors6

Flow rate(μL/min)12, 60, 120

Analysis speed10,000 events/sec

Sample input5mL Falcon Polystyrene tube (ref. 352052, 352054, 352235)

SoftwareBD FACSDiva software v6.2


Cell sorters

  • FACSAria IIIu – 15 color cell sorter

 

 

 

 

 

 

 

 

 

BrandBecton Dickinson

Available Excitation wavelength(nm)375, 404, 488, 561, 639

Available fluorescense detectors15

Sorting speed70,000 events/sec

Sample inputmicro tube, 5mL tube, 15mL tube

Collection way2-way, 4-way, plate and slide sorting

Collection deviceEppendorf, micro tube, 5mL tube, 15mL tube

                              6-384 well plate, slide

SoftwareBD FACSDiva software v6.2


  • FACSJazz – 6 color cell sorter

 

 

 

 

 

 

 

 

 

 

BrandBecton Dickinson

Available Excitation wavelength(nm)488, 561, 640

Available fluorescense detectors6

Sorting speed20,000 events/sec

Sample input5mL Falcon polypropylene tube (ref.352063)

Collection way:2-way、plate and slide sorting

Collection device:5mL tube, 15mL tube

                              6-384 well plate, slide

SoftwareBD FACSTM software


 

儀器設備

 

 


分析儀

 

  • LSRII SORP – 17色分析儀

 

 

 

 

 

 

 

 

 

廠牌:Becton Dickinson

可用激發光波長(nm)355, 404, 488, 561, 639

可偵測顏色數:17

上樣流速(μL/min)12, 24, 36, 60, 72, 120

分析速度:70,000 events/sec

上樣方式:5mL Falcon Polystyrene tube (ref. 352052, 352054, 352235), 96 well plate, 384 well plate

操作軟體:BD FACSDiva software v6.2


  • Attune CytPix – 14色影像式分析儀

 

 

 

 

 

 

 

 

 

廠牌:Thermo Fisher Scientific

可用激發光波長(nm)405, 488, 561, 637

可偵測顏色數:14

上樣流速(μL/min)12, 25, 100, 200, 500, 1000

分析速度:35,000 events/sec

上樣方式:eppendorff, micro tube, 5mL tube

自動上樣系統:eppendorff, 96孔盤, 384 孔盤 (標準或深底盤)

影像分析:明視野影像,不具螢光影像分析功能

鏡頭倍率:20X鏡頭 (0.45 NA)

影像解析度:0.3um/pixel

影像靈敏度:> 800nm particles

影像擷取速度:~ 6000張/秒

影像尺寸:96x96 (pixel) ~ 248x248 (pixel)

操作軟體:Attune software v5.2


  • Attune NxT – 14色分析儀

 

 

 

 

 

 

 

 

 

 

 

 

 

廠牌:Thermo Fisher Scientific

可用激發光波長(nm)405, 488, 561, 637

可偵測顏色數:14

上樣流速(μL/min)12, 25, 100, 200, 500, 1000

分析速度:35,000 events/sec

上樣方式:eppendorff, micro tube, 5mL tube

操作軟體:Attune software v4.2


  • FACSCanto – 6色分析儀

 

 

 

 

 

 

 

 

廠牌:Becton Dickinson

可用激發光波長(nm)488, 633

可偵測顏色數:6

上樣流速(μL/min)12, 60, 120

分析速度:10,000 events/sec

上樣方式:5mL Falcon Polystyrene tube (ref. 352052, 352054, 352235)

操作軟體:BD FACSDiva software v6.2


分選儀

  • FACSAria IIIu – 15色分選儀

 

 

 

 

 

 

 

 

 

廠牌:Becton Dickinson

可用激發光波長(nm)375, 404, 488, 561, 639

可偵測顏色數:15

分析速度:70,000 events/sec

上樣方式:micro tube, 5mL tube, 15mL tube

分選方式:2向、4向、盤式分選

樣本收集方式:Eppendorf, micro tube, 5mL tube, 15mL tube

                    6-384 well plate, slide

操作軟體:BD FACSDiva software v6.2


  • FACSJazz – 6色分選儀

 

 

 

 

 

 

 

 

 

 

廠牌:Becton Dickinson

可用激發光波長(nm)488, 561, 640

可偵測顏色數:6

分析速度:20,000 events/sec

上樣方式:5mL Falcon polypropylene tube (ref.352063)

分選方式:2向、盤式分選

樣本收集方式:5mL tube, 15mL tube

                    6-384 well plate, slide

操作軟體:BD FACSTM software


 


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