SR Lab

Antibodies

We have generated monoclonal antibodies against several antigens and cell types. These antibodies are available for commercialization and for collaborative research projects. sroff@ibms.sinica.edu.tw for more information.

Antibody Index

Antigens

PEG Polyethylene glycol

More information and references about the anti-PEG antibodies can be found here ANTI-PEG antibodies

AFP Alpha fetoprotein (human)

PE Pseudomaonas exotoxin A

PLC delta 1 Phospholipase C delta 1 (human)

PLC delta 3 Phospholipase C delta 3 (human)

bG beta-glucuronidase (E. coli)

hbG beta-glucuronidase (human)

mbG beta-glucuronidase (murine)

RC RNAse RNAse (Bullfrog)

topo I Topoisomerase I (human)

Muc5 (human mucin)

TSG101 Tumor suppressor gene 101 (human)

MA HIV-1 matrix protein (p17(Gag))

TAL6 Tumor antigen TAL6 (human)

Cells

Caski Human cervical carcinoma

AS-30D Rat hepatoma

B16/F10 Mouse metastatic melanoma

B16/BL6 Mouse metastatic melanoma

Human cells Various human tumor and normal cells

CL1-0 Low metastatic human lung adenocarcinoma

CL1-5 High metastatic human lung adenocarcinoma

Virus

JEV Japanese encephalitis E protein

micoplasma

Human alpha-fetoprotein

Name	Antigen	Subclass	Comments
AFP3.3	human AFP	IgG2a	Binds to epitope 1 of AFP
1E8-E7-A11	human AFP	IgG1	Binds to epitope 1 of AFP
8B3-B11-E6	human AFP	IgG2b	Binds to epitope 1 of AFP
2C1-A10-A2	human AFP	IgG1	Binds to epitope 2 of AFP
4G8-B1-D10	human AFP	IgG1	Binds to epitope 2 of AFP
85-G4-C11	human AFP	IgG1	Binds to epitope 2 of AFP
1C10-B10-C10	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
3E9-F12-C10	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
4C10-H2-D6	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
6C10-A8-C1	human AFP	IgG1	Binds to epitope 3 (not 1 or 2) of AFP
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Pseudomonas exotoxin A

Name	Antigen	Subclass	Comments
2G6-11B	PE		not characterized
2B11-9G	PE		"
3A2-7C	PE		"
3E1-4B	PE		"
1D12-C3	PE		"
4G1-1F	PE		"
2B1-4G	PE		"
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Phospholipase C delta 1

Name	Antigen	Subclass	Comments
G	recombinant human PLC delta 1	IgG1
1C9	"	IgG1
3E8	"	IgG1
3H5	"	IgG1
5G5	"	IgG1
6A6	"	IgG1
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Phospholipase C delta 3

Name	Antigen	Subclass	Comments
3G1-B1	recombinant human PLC delta 3		binds dominant C-teminal domain epitope
8B6-B9	"		"
9E4-G11	"		"
9E5-B6	"	IgG1	"
10B11-B5	"	IgG1	"
2A11-D10-D8	N-terminal domain of PLC delta3		binds N-terminal domain
8G10-C12-B12	"		"
9H5-F5-B10	"		"
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E. coli beta-glucuronidase

Name	Antigen	Subclass	Comments
1E8	recombinant E. coli beta-glucuronidase	IgG1	good for western blots, ELISA
3C1	"	IgG1
6D1	"	IgG2a
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human beta-glucuronidase

Name	Antigen	Subclass	Comments
1B3-C1	rhuman beta-G	IgG1 lambda	ELISA +++, Western +++, no cross rxn mouse beta-G, does not inhibit activity
1B5-D3	rhuman beta-G	IgG2b kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
3F2-G10	rhuman beta-G	IgG1 kappa	ELISA +++, Western +++, no cross rxn mouse beta-G, does not inhibit activity
3G7-H12	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
5E1-H4	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
5G11-F9	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
7E1-A12	rhuman beta-G	IgG1 kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
7F7-F3	rhuman beta-G	IgG2b lambda	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
7G8-B7	rhuman beta-G	IgG2b kappa	ELISA +++, Western -, no cross rxn mouse beta-G, does not inhibit activity
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murine beta-glucuronidase

Name	Antigen	Subclass	Comments
7G7	rmurine beta-G	rat IgG	ELISA +++, FACs +++, Western -, no cross rxn human beta-G, does not inhibit activity
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RC RNAse

Name	Antigen	Subclass	Comments
RC1	rRNAse	IgG1	reacts with native RC bullfrog RNAse
RC2	"	IgG1	"
RC3	"	IgG2b	"
RC4	"	IgG1	"
RC5	"	IgG1	"
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Topisomerase I

Name	Antigen	Subclass	Comments
SR-TOP-1A	rhuman topo I	IgM	ELISA +, Immunoblot +/-
SR-TOP-2A	"	IgM	ELISA +, Immunoblot +/-
SR-TOP-4A	"	IgG2b	ELISA +++, Immunoblot +++
SR-TOP-4C	"	IgG2b	ELISA +++, Immunoblot +++
4F2	"	ND	ELISA -, Immunoblot +
8D3	"	ND	ELISA ++, Immunoblot +
9F4	"	ND	ELISA -, Immunoblot +/-
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Muc 5

Name	Antigen	Subclass	Comments
5D5-G11	muc5	IgG1	reacts with human muc5 (mucin)
6E3-G10	"	IgG1	"
9B12-A2	"	IgG1	"
7D6-E6	"	IgG1	"
8A1-H2	"	IgG1	"
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Methoxy polyethylene glycol (PEG)
More info

Name	Antigen	Subclass	Comments
AGP3	bG-sPEG conjugate	IgM	Specific for PEG backbone
E11	"	IgG1	Specific for PEG backbone
AGP4	"	IgM	Specific for PEG backbone
3.3	"	IgG1	Specific for PEG backbone
6.3	"	IgG1	Specific for PEG backbone, high affinity
15-2b	"	IgG2b	Specific for methoxy terminus, high affinity
r33G	"	IgG1	rat mAb, specific for PEG backbone, high affinity
rAGP6	"	IgM	rat mAb, specific for PEG backbone, high affinity
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Tumor Suppressor Gene 101 protein (tsg101)

Name	Antigen	Subclass	Comments
2A8-F11	recombinant human tgs101		binds to domain between aa 167-374, perinuclear and diffuse cytoplasmic immunofluorescence staining
3D8-H6	"	IgG1	binds to domain between aa 250-300, perinuclear and diffuse cytoplasmic immunofluorescence staining
3G1-D11	"		perinuclear immunofluorescence staining
6B3-G1	"
7H5-D6	"
10H12-C6	"	IgG1
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HIV matrix protein (p17)

Name	Antigen	Subclass	Comments
6D1-H3	recombinant MA		p17 (gag) / HIV matrix protein
9G8-C5	"
9G12-D4	"
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Tumor Associated Antigen L6

Name	Antigen	Subclass	Comments
1F4	TAL6 DNA immunization	IgG1	flow cytometer/ELISA
3C6	"	IgG1	"
6D6	"	IgG1	"
8D10	"	IgM	"
9C7	"	IgG1	"
10A9	"	IgG1	"
10H6	"	IgM	Western blots
2E7	"	IgM	dot blots / may recognize cryptic or internal epitope
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Human cervical carcinoma

Name	Antigen	Subclass	Comments
1H10	CaSki cells	IgG3	reacts with human cervical and colon carcinoma
9D5	SiHa cells		reacts with some human cervical carcinomas
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Rat hepatoma AS-30D cells

Name	Antigen	Subclass	Comments
RH1	AS-30D cells	IgG2a	reacts withAS-30D rat hepatoma cells
1C6-6C	"	IgG2a	"
6C6-4G	"	IgG2a	"
3F6-9B	"	IgG2a	"
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B16/F10 melanoma

Name	Antigen	Subclass	Comments
3D5	B16/F10 cells	IgM	reacts with B16/F10 melanoma cells (C57/BL6 mice)
E8	"	IgG2b	"
E9	"	IgG2b	"
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B16/BL6 melanoma

Name	Antigen	Subclass	Comments
1E9	B16/BL6 cells	IgM	reacts with B16/BL6 metastatic melanoma cells (C57/BL6 mice)
3D8	"	IgM	"
3F10	"	IgM	"
6G9	"	IgM	"
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Human cells

Name	Antigen	Subclass	Comments
AC-1	human cells	IgG1	reacts with most human cells
AC-2	"	IgG1	"
AC-3	"	IgM	mAbs available up to AC-10
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Low metastatic lung adenocarcinoma (CL1-0)

Name	Antigen	Subclass	Comments
6D8-B2	CL1-0 human lung adenocarcinoma cells		preferentially binds CL1-0 more than CL1-5
8E6-C2	"		"
9F11-B1	"		"
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High metastatic lung adenocarcinoma (CL1-5)

Name	Antigen	Subclass	Comments
Ly-1F4	CL1-5 human lung adenocarcinoma cells	IgG1	binds CD13, partially inhibits aminopeptidase activity
Ly-1G9	"	IgG1	binds EphA2
Ly-2A2	"	IgG1	binds VLA-3 alpha (integrin alpha 3)
Ly-3C1	"	IgG1	binds EphA2
Ly-3E4	"	IgG1	preferentially binds CL1-5 more than CL1-0
Ly-3F7	"	IgG2b	binds EphA2
Ly-4B8	"	IgG2b	binds CD13, goes not inhibit aminopeptidase activity
Ly-4F5	"	IgG1	preferentially binds CL1-5 more than CL1-0
Ly-5D7	"	IgG1	"
Ly-6F10	"	IgG1	"
Ly-7F10	"	IgG1	"
Ly-8G6	"	IgG1	"
Ly-9A1	"	IgG1	binds CD13, partially inhibits aminopeptidase activity
Ly-9F7	"	IgG2b	preferentially binds CL1-5 more than CL1-0
Ly-10D5	"	IgG1	"
Ly-10F3	"	IgG1	"
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Japanese encephalitis virus

Name	Antigen	Subclass	Comments
5G11-G3-B5	JEV	IgG2b	binds JEV-E protein
5E11-D9-G6	"	IgG2b	"
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Micoplasma

Name	Antigen	Subclass	Comments
2BS	infected cells	IgG3	detects micoplasma infected cells
4BF	"	IgG1	"
5BFII	"	IgG3	"
6BF	"	IgG2b	"
7BF3	"	IgG1	"
8BF	"	IgG2b	"
10BFII	"	IgM	"
11BFII	"	IgM	"
12BFII	"	IgM	"
17BFII	"	IgM	"
18BFII	"	IgM	"
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