唐堂博士
副院長
特聘研究員
中央研究院院士
- 02-27899156(lab) (Lab) (Room No: 301)
- 02-26523901(o)
- 02-27829143 (Fax)
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Specialty:
- Centrosome and Cilia Biogenesis
- Mitosis
- Neural Stem Cell Division
Education and Positions:
Ph.D. Human Genetics, Yale University
中心粒/中心體，纖毛，神經幹細胞，與癌細胞分裂之研究
細胞中心體含有母、子二顆中心粒，主要是促成細胞纖毛，鞭毛的形成及參與細胞有絲分裂。畸型小頭症（MCPH）是一種人類遺傳疾病，主要症狀是病人腦容量小，並伴隨中，重度智力障礙。目前已知多個MCPH基因缺陷會造成畸型小頭症。近年來本研究室解開其中4個畸型小頭症蛋白CPAP, STIL, CEP135, 和RTTN的功能。本研究室發現STIL（EMBO J, 2011)， CEP135 (EMBO J, 2013) 都會與CPAP蛋白（Nat Cell Biol 2009）結合，且RTTN亦會與STIL結合（Nat Commun 2017, Cells 2021），形成多個蛋白質複合體，共同參與中心粒的複製與細胞分裂。有趣的是CPAP蛋白缺失會誘發神經幹細胞死亡，神經細胞過早分化，及嚴重腦部病變 （J Cell Sci 2020）。據此我們提出一個假設：干擾細胞中心粒複製，將會抑制神經幹細胞分裂與減少大腦皮層神經細胞數目，繼而造成人類畸型小頭症。另外本研究室亦發現CEP120 (J Cell Biol, 2013; Sci Rep, 2019)與CEP295 (J Cell Sci 2016)蛋白會調控中心粒長度，且Myosin-Va在纖毛起始過程中扮演一非常重要的角色：Myosin-Va會將preciliary vesicles首先帶到母中心粒上，繼而開啟了纖毛形成初始機轉(Nat Cell Biol 2018)。近年來癌細胞發現有基因體不穩定現象，此有可能是中心體複製不正常所致。未來本研究室將利用分子，細胞，遺傳，小鼠模式，及hiPSC-derived類人腦器官之技術來探討中心體/中心粒及纖毛形成機轉，並探討干擾此機轉所可能誘發人類畸型小頭症，纖毛病變，及癌基因體不穩定之病因。
Our Team
期刊 60
專書 1
期刊 60 專書 1
- Wang YW, Chen SC, Gu DL, Yeh YC, Tsai JJ, Yang KT, Jou YS, Chou TY, Tang TK* A novel HIF1alpha-STIL-FOXM1 axis regulates tumor metastasis. Journal of biomedical science 29(1), 24 (2022-04) [JCR] [WOS]
- An HL, Kuo HC, Tang TK* Modeling Human Primary Microcephaly With hiPSC-Derived Brain Organoids Carrying CPAP-E1235V Disease-Associated Mutant Protein Frontiers in Cell and Developmental Biology 10, 1-18 (2022-03) [JCR] [WOS]
- Chang CH, Chen TY, Lu IL, Li RB, Tsai JJ, Lin PY, Tang TK* CEP120-mediated KIAA0753 recruitment onto centrioles is required for timely neuronal differentiation and germinal zone exit in the developing cerebellum. Genes & development 35(21-22), 1445-1460 (2021-11) [JCR] [WOS]
- Chou EJ, Tang TK* Human Microcephaly Protein RTTN Is Required for Proper Mitotic Progression and Correct Spindle Position Cells 10(6), 1441 (2021-06) [JCR] [WOS]
- Chen TY, Huang BM, Tang TK, Chao YY, Xiao XY, Lee PR, Yang LY, Wang CY Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response. Cell death and differentiation 28, 1865–1879 (2021-01) [JCR] [WOS]
- Lin YN, Lee YS, Li SK, Tang TK* Loss of CPAP in developing mouse brain and its functional implication in human primary microcephaly. Journal of cell science 133(12), 1-14 (2020-06) [JCR] [WOS]
- Tsai JJ, Hsu WB, Liu JH, Chang CW, Tang TK* CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis. Scientific reports 9(1), 6037 (2019-04) [JCR] [WOS]
- Wu CT, Chen HY, Tang TK* Myosin-Va is required for preciliary vesicle transportation to the mother centriole during ciliogenesis. Nature cell biology 20(2), 175-185 (2018-01) [JCR] [WOS]
- Wang ZH, Chang YY, Wu JG, Lin CY, An HL, Luo SC, Tang TK, Su WF Novel 3D Neuron Regeneration Scaffolds Based on Synthetic Polypeptide Containing Neuron Cue Macromolecular Bioscience 1700251, 1-12 (2017-12) [JCR] [WOS]
- Chen HY, Wu CT, Tang CC, Lin YN, Wang WJ, Tang TK* Human microcephaly protein RTTN interacts with STIL and is required to build full-length centrioles. Nature communications 8(1), 247 (2017-08) [JCR] [WOS]