謝如姬博士

研究員
Specialty:
  • Biophysics/Electrophysics
  • Fluorescence/Ion Chennel
  • Thermodynamics
Education and Positions:

  • Ph.D. University of Rochester

離子通道是位於細胞膜上的蛋白質，負責運輸細胞內外一些特定的離子產生電流並造成細胞內外電位差。這種電位差可快速又精確地在細胞之間傳遞訊息。內向整流性的鉀通道控制細胞興奮性並進而調整許多生理功能，譬如心跳速率、腎臟鹽份的代謝及胰導素之分泌。 本實驗室結合電生理、定點突變技術及單一通道的開關分析探討內向整流性的鉀通道的基本性質如通道的開關和通道的導電性並將基礎研究得到的結果運用到生理功能及病理治療(如心律不整)的探討。此外，我們也運用熱力學的原理探討離子通道中不同區域的交互作用及其產生的能量。另一方面，我們正在研發可以被光激活的鉀離子及鈣離子通道。當我們成功地製造出此二類通道後，我們就能藉由光來調控神經及心臟細胞電訊號的開與關，進而控制這些細胞的功能以及瞭解細胞間的溝通網路。我們預期，這些研究可以發展出新的學術探討利器，並且提供一些疾病如癲癇、心臟衰竭、心律不整的治療新方法。

Our Team
Team photo

期刊 23 專書 0

  1. Sigg DM, Chang HK, Shieh RC Linkage analysis reveals allosteric coupling in Kir2.1 channels. The Journal of general physiology 150(11), 1541-1553 (2018) [JCR] [WOS]
  2. Liu CH, Chang HK, Lee SP, Shieh RC Activation of the Ca2+-sensing receptors increases currents through inward rectifier K+ channels via activation of phosphatidylinositol 4-kinase. Pflugers Archiv : European journal of physiology 468(11-12), 1931-1943 (2016) [JCR] [WOS]
  3. Chang HK, Iwamoto M, Oiki S, Shieh RC Mechanism for attenuated outward conductance induced by mutations in the cytoplasmic pore of Kir2.1 channels. Scientific reports 5, 18404 (2015) [JCR] [WOS]
  4. Hsueh-Kai Chang and Ru-Chi Shieh Voltage-dependent inhibition of Kir2.1 outward currents by extracellular spermine BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1828, 765-775 (2013-02) [JCR] [WOS]
  5. T-A Liu; H-K Chang; (R-C Shieh*) . Revisiting Inward Rectification: K Ions Permeate through Kir2.1 Channels during High-Affinity Block by Spermidine. JOURNAL OF GENERAL PHYSIOLOGY 139, 245-259 (2012-02) [JCR] [WOS]
  6. T-A Liu; H-K Chang; (R-C Shieh*) Extracellular K+ elevates outward currents through Kir2.1 channels by increasing single-channel conductance. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1808(6), 1772-1778 (2011-06) [JCR] [WOS]
  7. Hsueh-Kai Chang, Jay-Ron Lee, Tai-An Liu, Ching-Shu Suen, Jorge Arreola, and Ru-Chi Shieh* The extracellular K+ concentration-dependence of outward currents through Kir2.1 channels is regulated by extracellular Na+ and Ca2+. JOURNAL OF BIOLOGICAL CHEMISTRY 285(30), 23115-23125 (2010) [JCR] [WOS]
  8. Kun-Chi Chiang, Ling-Ping Lai, and *Ru-Chi Shieh** Characterization of a novel Nav1.5 channel mutation, A551T, associated with Brugada syndrome. JOURNAL OF BIOMEDICAL SCIENCE 16, 76 (2009) [JCR] [WOS]
  9. Hsueh-Kai Chang, Laurence J. Marton, Kuo Kan Liang, and *Ru-Chi Shieh K^+ binding in the G-loop and water cavity facilitates Ba^2+ movement in the Kir2.1 channel. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1788, 500-506 (2009) [JCR] [WOS]
  10. Hsuan-Wen Hwang, Jay-Ron Lee, Kuan-Yu Chou, Ching-Shu Suen, Ming-Jing Hwang, Chinpan Chen, *Ru-Chi Shieh*, Lee-Young Chau** Oligomerization is crucial for the stability and function of heme oxygenase-1 in the endoplasmic reticulum. JOURNAL OF BIOLOGICAL CHEMISTRY 284(34), 22672-22679 (2009) [JCR] [WOS]
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