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黃怡萱博士

研究員
Division Chief
  • 02-2789-9174 (Lab) (Room No: N703)
  • 02-2652-3523 (Office)

Specialty:
  • Translational Control/ RNA 轉譯調控
  • Cap modification/ RNA-cap 修飾調控
  • Molecular & Cellular Neuroscience/ 分子與細胞神經生物學

Education and Positions:
  • Ph.D. University of Texas Southwestern Medical Center at Dallas


記憶如何形成和儲存在我們的腦中,是個有趣卻看似抽象的生物問題。隨著日新月異的技術和基因工程動物的發展,我們逐漸開始了解它的分子機制。在動物的中樞神經系統,神經細胞靠著特有的突觸結觸彼此傳遞訊息。每個突觸是個有塑性的訊息收發個體,會隨著所收到的訊息強度和次數而改變自己的結構,功能及數目,因而加強或減弱特定訊息的迴路。這種突觸可塑性是記憶形成的分子機轉。我們已知若要形成長久性的記憶,需要合成新的蛋白來維持突觸的結構改變。所以這些新合成的蛋白質被認為是組成記憶的分子體。細胞常藉由一群會和核醣核酸結合的蛋白 (RNA-binding proteins) 來選擇性的調控特定RNA的轉譯及蛋白的產生,因此,我們選擇了CPEB家族蛋白。希望藉由瞭解其在神經中的功能,去找出和記憶有關的蛋白分子體。

 

我們的另一研究重點是探討所知極少的帽甲基轉移酶的生理功能。近期的研究發現,轉錄後的基因調控,不僅取決於訊息核醣核酸 (mRNA) 的序列,還取決其核糖和鹼基的化學修飾。在所有真核生物中,mRNA的加帽修飾 (capping),可保護其免於降解並促進其轉譯。除了酵母菌僅具有原始的m7G帽 (cap0),其他高級真核生物mRNA的第一和第二核苷酸,可分別被帽甲基轉移酶CMTR1和CMTR2在2'-O-核糖位置甲基化。通常認為cap1結構 (m7GpppNmN) 可以避免引發先天免疫反應。但是,我們發現CMTR1對於腦發育的重要性是透過調控基因表達而非是防止先天免疫反應。

Our Team
Team photo

期刊 64 專書 0

  1. Chao YW, Lee YL, Tseng CS, Wang LU, Hsia KC, Chen H, Fustin JM, Azeem S, Chang TT, Chen CY, Kung FC, Hsueh YP*, (Huang YS)*, Chao HW* Improved CaP Nanoparticles for Nucleic Acid and Protein Delivery to Neural Primary Cultures and Stem Cells. ACS nano 18(6), 4822-4839 (2024) [JCR] [WOS]
  2. (Huang YS)*, Mendez R*, Fernandez M, Richter JD* CPEB and translational control by cytoplasmic polyadenylation: impact on synaptic plasticity, learning, and memory. Molecular psychiatry (2023) [JCR] [WOS]
  3. Tseng CS, Chao YW, Liu YH, (Huang YS)*, Chao HW* Dysregulated proteostasis network in neuronal diseases. Frontiers in cell and developmental biology 11, 1075215 (2023) [JCR] [WOS]
  4. Tsukamoto Y, Hiono T, Yamada S, Matsuno K, Faist A, Claff T, Hou J, Namasivayam V, Vom Hemdt A, Sugimoto S, Ng JY, Christensen MH, Tesfamariam YM, Wolter S, Juranek S, Zillinger T, Bauer S, Hirokawa T, Schmidt FI, Kochs G, Shimojima M, (Huang YS), Pichlmair A, Kummerer BM, Sakoda Y, Schlee M, Brunotte L, Muller CE, Igarashi M, Kato H Inhibition of cellular RNA methyltransferase abrogates influenza virus capping and replication. SCIENCE 379(6632), 586-591 (2023) [JCR] [WOS]
  5. Winkler I, Engler JB, Vieira V, Bauer S, Liu YH, Di Liberto G, Grochowska KM, Wagner I, Bier J, Bal LC, Rothammer N, Meurs N, Egervari K, Schattling B, Salinas G, Kreutz MR, (Huang YS), Pless O, Merkler D, Friese MA MicroRNA-92a-CPEB3 axis protects neurons against inflammatory neurodegeneration. Science advances 9(47), eadi6855 (2023) [JCR] [WOS]
  6. Wu CS, Lu WH, Hung MC, (Huang YS)*, Chao HW* From polyploidy to polyploidy reversal: its role in normal and disease states. Trends in genetics : TIG 38(10), 991-995 (2022) [JCR] [WOS]
  7. Lai HH, Hung LY, Yen CJ, Hung HC, Chen RY, Ku YC, Lo HT, Tsai HW, Lee YP, Yang TH, Chen YY, Huang YS, Huang W NEIL3 promotes hepatoma epithelial-mesenchymal transition by activating the BRAF/MEK/ERK/TWIST signaling pathway. The Journal of pathology 258(4), 339-352 (2022) [JCR] [WOS]
  8. Huang DF, Lee CY, Chou MY, Yang TY, Cao X, Hsiao YH, Wu RN, Lien CC, (Huang YS), Huang HP, Gau SS, Huang HS Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons. Proceedings of the National Academy of Sciences of the United States of America 119(33), e2203632119 (2022) [JCR] [WOS]
  9. Lu WH, Chang YM, (Huang YS*) Alternative Polyadenylation and Differential Regulation of Ucp1: Implications for Brown Adipose Tissue Thermogenesis Across Species. Frontiers in pediatrics 8, 612279 (2021) [JCR] [WOS]
  10. Lu WH, Chao HW, Lin PY, Lin SH, Liu TH, Chen HW, (Huang YS*) CPEB3-downregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice Neuropsychopharmacology 46, 1669-1679 (2021) [JCR] [WOS]

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