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賴時磊博士

助研究員
  • 886-2-27899019 (N611) (Lab)
  • 886-2-26523057 (Office)
Specialty:
  • Cardiovascular development and regeneration
  • Cardioimmunology: Inflammation and Immunity in Cardiovascular Disease
  • Developmental genetics in fish models for human diseases
Education and Positions:

  • Ph.D., National Taiwan University

    Postdoc, National Taiwan University

    Postdoc, Max Planck Institute for Heart and Lung Research

人類心肌梗塞導致心肌壞死後(俗稱心臟病)，死亡的心肌細胞無法再生。取而代之的結締組織會影響心臟功能、進一步導致心室肥大、心臟衰竭，與患者死亡；不同於哺乳類，許多演化上較原始的脊椎生物例如部分魚類與兩生類，在心肌壞死後仍保有再生能力，能夠完美的恢復組織型態與功能。

本實驗室以系統生物學與功能性基因體學的方式，結合多種模式生物探討脊椎動物心臟受損後的修復與再生機制。其中，斑馬魚與稻田魚同為淡水硬骨魚，具有相同的解剖構造、生理條件與生活環境。令人意外地，稻田魚在心肌受損後卻無法活化心肌再生。基於哺乳類與魚類在各方面的巨大差異，稻田魚與斑馬魚的對比提供絕佳的模式來探討心臟再生的關鍵因子。目前主要的研究方向是比較分析斑馬魚與稻田魚在組織受損後的基因轉錄反應(transcriptome)有何不同，以期增進我們對心臟再生的了解並進而轉譯這些知識到臨床治療，改善人類心臟病的預後。

我們的初步研究發現免疫反應與組織血管新生在這兩種模式生物中有明顯的不同。其中，巨噬細胞(macrophage)在這兩種模式生物的比較中扮演非常重要且不同的功能。在斑馬魚心臟受損時，巨噬細胞的入侵速度與數量似乎比在稻田魚心臟受損時快且多。減緩巨噬細胞在心臟受損後入侵傷處的速度竟會完全阻礙斑馬魚的心臟再生能力，即使晚到的巨噬細胞能夠達到一樣多的數目，暗示其功能特性已改變(polarity)；反之，簡單的注射免疫觸發物poly I:C竟能夠有效的活化巨噬細胞的入侵、並促進稻田魚的心臟再生。我們對這樣的結果感到非常興奮，並將積極地進一步探討背後的機制、以及轉譯這些知識到臨床治療。

Our Team
Team photo

期刊 16 專書 0

  1. Wei Ke-Hsuan, Lin I-Ting, Chowdhury Kaushik, Lim Khai Lone, Liu Kuan-Ting, Ko Tai-Ming, Chang Yao-Ming, Yang Kai-Chien, Lai Shih-Lei (Ben) Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration eLife 12, e84679 (2023-07) [JCR] [WOS]
  2. Chowdhury Kaushik, Lin Shi, Lai Shih-Lei Comparative Study in Zebrafish and Medaka Unravels the Mechanisms of Tissue Regeneration Frontiers in Ecology and Evolution 10, 1 (2022) [JCR] [WOS]
  3. Chowdhury K, Lai SL, Marin-Juez R Modulation of VEGFA Signaling During Heart Regeneration in Zebrafish. Methods in molecular biology (Clifton, N.J.) 2475, 297-312 (2022) [JCR] [WOS]
  4. Tsao KC, Lin YC, Chen YT, Lai SL, Yang RB Zebrafish scube1 and scube2 cooperate in promoting Vegfa signaling during embryonic vascularization. Cardiovascular research cvab, 125 (2022) [JCR] [WOS]
  5. Tsedeke AT, Allanki S, Gentile A, Jimenez-Amilburu V, Rasouli SJ, Guenther S, Lai SL, Stainier DYR, Marin-Juez R Cardiomyocyte heterogeneity during zebrafish development and regeneration. Developmental biology 476, 259-271 (2021) [JCR] [WOS]
  6. El-Brolosy MA, Kontarakis Z, Rossi A, Kuenne C, Gunther S, Fukuda N, Kikhi K, Boezio GLM, Takacs CM, Lai SL, Fukuda R, Gerri C, Giraldez AJ, Stainier DYR Genetic compensation triggered by mutant mRNA degradation. Nature 568(7751), 193-197 (2019-04) [JCR] [WOS]
  7. Lai SL, Marin-Juez R, Stainier DYR Immune responses in cardiac repair and regeneration: a comparative point of view. Cellular and molecular life sciences : CMLS 76(7), 1365-1380 (2019-04) [JCR] [WOS]
  8. Burg L, Palmer N, Kikhi K, Miroshnik ES, Rueckert H, Gaddy E, MacPherson Cunningham C, Mattonet K, Lai SL, Marin-Juez R, Waring RB, Stainier DYR, Balciunas D Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish. PLoS genetics 14(11), e1007754 (2018) [JCR] [WOS]
  9. Lai SL, Marin-Juez R, Moura PL, Kuenne C, Lai JKH, Tsedeke AT, Guenther S, Looso M, Stainier DY Reciprocal analyses in zebrafish and medaka reveal that harnessing the immune response promotes cardiac regeneration. eLife 6, e25605 (2017) [JCR] [WOS]
  10. Marin-Juez R, Marass M, Gauvrit S, Rossi A, Lai SL, Materna SC, Black BL, Stainier DY Fast revascularization of the injured area is essential to support zebrafish heart regeneration. Proceedings of the National Academy of Sciences of the United States of America 113(40), 11237-11242 (2016) [JCR] [WOS]
- More Publications-

- 博士後研究 -
Lin, I-Ting PhD
林奕廷
- 研究助理 -
Chen, Mei-Hua
陳美華
Huang, Chia-Lin
黃嘉琳
Liu, Hsing-Wei
劉行瑋
Lee, Ann Nee
李艾妮
Jian, Yi-Fei
簡翊霏
Nien, Shao-Fu Hunter
粘少輔
- 學生 -
Chowdhury, Kaushik
高希
Lang, Wei-Han Phillip
郎偉涵
Lim, Khai Lone
林凱倫
Hung, Yu-Jen Elly
洪鈺荏
Thu, Aye Myat
歐陽英英
- 訪問學人 -
Ma, Wei-Ning
馬韡寧
- 昔日夥伴 -
Wei, Ke-Hsuan (Leise)
魏可軒
Chen, Yu-Siang (Sean)
陳鈺翔
Chu, An-Ju (Annie)
朱安如
Chiu, Zheng-Wen
邱政文
Tamayanti, Wahyu Dewi
王仙渼
Chen, Vincent
陳政玄
Yungca, Mica Xiena
Yungca Mica Xiena
Cheng, Evelyn
鄭心謙
Lin, Chia-Hao
林家豪
Du, Hung-Yi Isaac
杜泓逸
Lee, Jung-Che Jerry
李鎔哲
Lin, Hao-Lin Jennifer
林浩琳
Lin, Shi
林昕
Lee, Po-I (Samson)
李柏毅
Cheng, Sophia
鄭淳溱
Chen, Mei-Lin
陳美琳
Naeem, Muhammad
穆哈瑪
Guo, Yi-Ting
郭怡葶
Chao, Yi-Ya (Vivian)
趙怡雅
Yen, Ju-Ching (Emily)
顏如靖
Yang, Hui-Ting
楊惠婷
Duan, Chung-Hsing
段中興
Zheng, Jun-Chung (Kevin)
鄭鈞中
Huang , Yu-Wen
黃鈺雯
Phillip, Iquo Oyohosuho
菲伊可
Ahmed, Hossam
艾禾桑
Wu, Jun-Yuan
吳俊淵
MAHMOUD, EL-HASSAN Moustafa
馬哈珊

  • Dean's Award from National Taiwan University
  • Speedy Horse Award from National Science Council (Visiting scientist fellowship/千里馬計畫) 
  • Postdoctoral Fellowship from National Science Council
  • Postdoctoral Fellowship from Max-Planck-Gesellschaft
  • Special outstanding talent award from the Ministry of Science and Technology (延攬特殊優秀人才)
  • Investigator Fellowship from Academia Sinica (新聘學術研究獎)
  • 8th Annual Excellence in Creativity Award for Young Scholar from The Foundation for the Advancement of Outstanding Scholarship (傑出人才發展基金會-年輕學者創新獎)
  • Excellent Young Scholar Grant from the Ministry of Science and Technology (科技部優秀年輕學者) 
  • Career Development Award from Academia Sinica (前瞻計畫)