Dr. Wang, Shu-Ping 's publons link picture

王書品博士

助研究員
  • 02-2789-9122 (Lab) (Room No: 540)
  • 02-2652-3073 (Office)
Specialty:
  • Molecular oncology, Cancer epigenetics
  • Biochemically defined cell free system
  • CRISPR-based genetic/epigenetic editing
Education and Positions:
    • B.S. National Sun Yat-Sen University
    • M.S. National Tsing-Hua University
    • Ph.D. National Defense Medical Center
    • Postdoc. The Rockefeller University, New York

研究興趣與目標

本實驗室的長期研究興趣是闡明與基本生物學問題（包括發育缺陷和癌症）相關的表觀遺傳調控機制。研究重點是破譯關鍵的表觀遺傳調節因子，包括組蛋白甲基轉移酶 (HMTs) 和去甲基化酶，如何在生理和疾病條件下通過增強子景觀修飾來控制目標基因的轉錄。對於所有提議的研究計劃，我們的最終目標是利用基於 CRISPR 的表觀基因組編輯技術和小分子誘導劑/抑製劑來精確操縱基因表達程序，從而確定細胞類型規範或糾正癌症疾病中錯誤調節的基因表達。

 

研究方向和項目

確立 MLL3/4甲基轉移酶為抗腫瘤標靶治療之靶標

儘管癌症基因組測序數據支持 MLL3 或 MLL4 在許多人類癌症中可能扮演關鍵之腫瘤抑制角色，但在某些情況下，這些組蛋白修飾酶也可能作為致癌輔助因子，並參與驅動腫瘤發生之過程。在這個研究主題中，我們想闡明某些癌症類型中潛在的 MLL3/4 致癌功能，確定 MLL3/4 為抗腫瘤標靶治療之靶標，並設計小分子抑製劑作為這些癌症的潛在靶向治療。

 

探討H3K4 甲基轉移酶/去甲基化酶基軸調節癌症進展

雖然 H3K4 三甲基化 (H3K4me3) 在主要修飾啟動子以正向調節基因轉錄，但最近的研究表明H3K4三甲基化同時也具有激活增強子的潛在功能。KDM5C H3K4去甲基酶複合物可以將大多數基因組上的增強子的活性維持在正常水平; 然而當這種 H3K4 去甲基酶複合物失能，則會大幅地增加增強子上的 H3K4三甲基化，強化增強子活性，進而引發致癌基因的過度表現並促使癌症進展。在這個研究項目中，我們想確定負責增強子H3K4三甲基化的關鍵H3K4甲基轉移酶，並闡明此新穎的H3K4甲基轉移酶/去甲基酶基軸作用於癌症進展中的潛在分子機制。

 

繪製致癌基因和抑癌基因的增強子調控元件

增強子包含各式各樣的DNA序列，這些序列對於精確的基因調控至關重要，並且可以通過與目標啟動子形成環形結構而與之進行溝通。重要的是，增強子功能發生障礙會導致目標致癌基因或抑癌基因的異常表現，從而誘發腫瘤發生。因此，全面了解致癌基因與腫瘤抑制基因相關的增強子序列和其在基因組的位置將有利於癌症表觀基因組分析。在這個研究方向，我們希望建立一套分析與鑑定系統，通過 ChIP 測序、CRISPR 干擾 (CRISPRi) 和 4C（染色質空間構象捕捉）測序技術，鑑定並分析癌症表觀基因組中關鍵增強子及其目標致癌或抑癌基因之相關性。

Our Team
Team photo

期刊 17 專書 0

  1. Vikrant Kumar Sinha,Yi Zhang, Longxia Xu, Yu-Wen Chen, Sarah Picaud, Mohamad Zandian, Soumi Biswas, Panagis Filippakopoulos, (Shu-Ping Wang), Xiaobing Shi, Tatiana G. Kutateladze* Histone H4K16ac binding function of the triple PHD finger cassette of MLL4 Journal of Molecular Biology 10.1016/, j.jmb.-2023.168212 (2023-07-20) [JCR] [WOS]
  2. Hsiang-Chi Huang, Shih-Han Wang, Guo-Chen Fang, Wen-Cheng Chou, Chun-Che Liao, Cheng-Pu Sun, Jia-Tsrong Jan, Hsiu-Hua Ma, Hui-Ying Ko, Yi-An Ko, Ming-Tsai Chiang, Jian-Jong Liang, Chun-Tse Kuo, Te-An Lee, Diego Morales-Scheihing, Chen-Yang Shen, Shih-Yu Chen, Louise D. McCullough, Lu Cui, Gerlinde Wernig, Mi-Hua Tao, Yi-Ling Lin, Yao-Ming Chang*, (Shu-Ping Wang*), Yun-Ju Lai*, Chia-Wei Li*. Upregulation of PD-L1 by SARS-CoV-2 promotes immune evasion JOURNAL OF MEDICAL VIROLOGY 2023;, 95:-e28478. (2023-01-07) [JCR] [WOS]
  3. Binay K. Sahoo, Yuh-Charn Lin, Cheng-Fen Tu, Chien-Chin Lin, Wei-Ju Liao, Fu-An Li, Ling-Hui Li, Kurt Yun Mou, Steve R. Roffler, (Shu-Ping Wang), Chi-Tai Yeh, Chi-Yuan Yao, Hsin-An Hou, Wen-Chien Chou, Hwei-Fang Tien and Ruey-Bing Yang. Signal peptide-CUB-EGF-like repeat-containing protein 1-promoted FLT3 signaling is critical for the initiation and maintenance of MLL-rearranged acute leukemia Haematologica DOI:, 10.3324-/haematol.2022.281151 (2022-08-25) [JCR] [WOS]
  4. Lan-Hsin Wang, Marvin Angelo E. Aberin, Sean Wu, (Shu-Ping Wang)* The MLL3/4 H3K4 methyltransferase complex in establishing an active enhancer landscape BIOCHEMICAL SOCIETY TRANSACTIONS 49 (3), 1041-1054 (2021-06-22) [JCR] [WOS]
  5. Chia-Sing Lu, Ching-Wen Lin, Ya-Hsuan Chang, Hsuan-Yu Chen, Wei-Chia Chung, Wei-Yun Lai, Chao-Chi Ho, Tong-Hong Wang, Chi-Yuan Chen, Chen-En Yeh, Sean Wu, (Shu-Ping Wang)*, Pan-Chyr Yang*. Antimetabolite Pemetrexed Primes a Favorable Tumor Microenvironment for Immune Checkpoint Blockade Therapy JOURNAL FOR IMMUNOTHERAPY OF CANCER 8, e001392 (2020-11-26) [JCR] [WOS]
  6. Ting-Chun Kuo, Kuo-Yen Huang, Shuenn-Chen Yang, Sean Wu, Wei-Chia Chung, Yih-Leong Chang, Tse-Ming Hong, (Shu-Ping Wang), Hsuan-Yu Chen, Tzu-Hung Hsiao, Pan-Chyr Yang Monocarboxylate Transporter 4 Is a Therapeutic Target in Non-small Cell Lung Cancer with Aerobic Glycolysis Preference Molecular Therapy - Oncolytics 18, 189-201 (2020-09) [JCR] [WOS]
  7. Liling Wan, Shasha Chong, Fan Xuan, Angela Liang, Xiaodong Cui, Leah Gates, Thomas S. Carroll, Yuanyuan Li, Lijuan Feng, Guochao Chen, (Shu-Ping Wang) , Michael V. Ortiz, Sara K. Daley, Xiaolu Wang, Hongwen Xuan, Alex Kentsis, Tom W. Muir, Robert G. Roeder, Haitao Li, Wei Li, Robert Tjian, Hong Wen, C. David Allis Impaired cell fate through gain-of-function mutations in a chromatin reader Nature 577(7788), 121-126 (2020-01) [JCR] [WOS]
  8. Yi Zhang , Younghoon Jang, Ji-Eun Lee, JaeWoo Ahn, Longxia Xu, Michael R. Holden, Evan M. Cornett, Krzysztof Krajewski, Brianna J. Klein, (Shu-Ping Wang), Yali Dou, Robert G. Roeder, Brian D. Strahl, Scott B. Rothbart, Xiaobing Shi, Kai Ge*, Tatiana G. Kutateladze*. Selective binding of the PHD6 finger of MLL4 to histone H4K16ac links MLL4 and MOF NATURE COMMUNICATIONS 10, 2314 (2019-05-24) [JCR] [WOS]
  9. Xiaomu Wei, M. Nieves Calvo-Vidal, Siwei Chen, Gang Wu, Maria V. Revuelta, Jian Sun, Jinghui Zhang, Michael F. Walsh, Kim E. Nichols, Vijai Joseph, Carrie Snyder, Celine M. Vachon, James D. McKay, (Shu-Ping Wang), David S. Jayabalan, Lauren M. Jacobs, Dina Becirovic, Rosalie G. Waller, Mykyta Artomov, Agnes Viale, Jayeshkumar Patel, Jude Phillip, Selina Chen-Kiang, Karen Curtin, Mohamed Salama, Djordje Atanackovic, Ruben Niesvizky, Ola Landgren, Susan L. Slager, Lucy A. Godley, Jane Churpek, Judy E. Garber, Kenneth C. Anderson, Mark J. Daly, Robert G. Roeder, Charles Dumontet, Henry T. Lynch, Charles G. Mullighan, Nicola J. Camp, Kenneth Offit, Robert J. Klein, Haiyuan Yu, Leandro Cerchietti, Steven M. Lipkin* Germline Lysine-Specific Demethylase 1 (LSD1/KDM1A) Mutations Confer Susceptibility to Multiple Myeloma Cancer Research 78(10), 2747-2759 (2018) [JCR] [WOS]
  10. Leah A. Gates, Guowei Gu, Yue Chen, Aarti D. Rohira, Jonathan T. Lei, Ross A. Hamilton, Yang Yu, David M. Lonard, Jin Wang, (Shu-Ping Wang), David G. Edwards, Philip F. Lavere, Jiangyong Shao, Ping Yi, Antrix Jain, Sung Yun Jung, Anna Malovannaya, Shunqiang Li, Jieya Shao, Robert G. Roeder, Matthew J. Ellis, Jun Qin, Suzanne A. W. Fuqua, Bert W. O’Malley, Charles E. Foulds* Proteomic profiling identifies key coactivators utilized by mutant ERα proteins as potential new therapeutic targets Oncogene 37(33), 4581-4598 (2018) [JCR] [WOS]
- More Publications-

- 博士後研究 -
Chen, Yu-Wen
陳譽文
Hong, Hsiao-Chin
洪曉琴
- 學生 -
Liu, Ta-Yu
劉大猷
Aberin, Marvin
馬文
Khag, Joy
喬伊
Yeh, Lin-Wen
葉齡文
Wu, Sean
吳泓賢
Yeh, Sheryl
葉佳芸
Chen, Je-Wei
陳哲緯
Rashid, Mariya
龍儷雅
Lin, Ting-Jun
林庭君
Chang, Pei-Chi
張倍綺
- 昔日夥伴 -
Chung, Pei-Chi
鐘珮綺
Pak, Yao-Ying
畢曜瀅