張耀明博士
研究助技師
- 2652-3083 (Office)
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Specialty:
Computational Biology
Transcriptome Analysis
Gene Regultory Network
Education and Positions:
Ph.D. in Computer Science and Information Engeering (National Taiwan Normal University)
針對最新的生物技術所產出的資料，本實驗室致力於發展合適的分析方法以用回答重要的生物醫學問題。近年來，蓬勃發展的高通量和酸定序的技術對於基因等遺傳物質的了解提供了全面且精準的解決方案。許多定序技術（如WGS, RNA-seq, ChIP-seq, ATAC-seq等等）目前已經被大量使用。由於比較時間序列的RNA-seq資料分析可以幫助生物學家們解開細胞裡正在發生的程序與基因的調控路徑，我們克服了分析這類資料中必須同時考慮基因表現、時間順序、與不同實驗之間的因素，發展建立了一套時序基因共表現網路（TO-GCN）方法。
這個方法已經應用在許多研究上：如人類間質幹細胞的發育、斑馬魚心臟受損再生、小鼠胚胎心臟發育、SARS-CoV-2感染人類細胞過程等，我們希望可以透過更多的合作研究，幫助生醫學者在重要或是有趣的問題上有機會可以找出前人一直以來無法得到的答案。
有鑒於單細胞核酸定序技術越來越普及，建構在原本TO-GCN的概念基礎上，我們也正發展適合分析單細胞定序資料的新方法，將分析的解析度從原本的組織層級一舉推升到細胞型態層級，分析結果更能反應真實的情況。
期刊 27
專書 0
期刊 27 專書 0
- Huang FL, (Chang YM), Lin CY, Yu SJ, Fu JT, Chou TY, Yeh SW, Liao EC, Li CL* Regulating TKT activity inhibits proliferation of human acute lymphoblastic leukemia cells American journal of cancer research 14(2), 679-695 (2024-02-15) [JCR] [WOS]
- Chou Wen-Cheng, Chen Wei-Ting, Kuo Chun-Tse, (Chang Yao-Ming), Lu Yen-Shen, Li Chia-Wei, Hung Mien-Chie, Shen Chen-Yang* Genetic insights into carbohydrate sulfotransferase 8 and its impact on the immunotherapy efficacy of cancer Cell Reports 43(1), 113641 (2024-01-23) [JCR] [WOS]
- Siew Jian Jing, Chen Hui-Mei, Chiu Feng-Lan, Lee Chia-Wei, (Chang Yao-Ming), Chen Hung-Lin, Nguyen Thi Ngoc Anh, Liao Hung-Ting, Liu Mengyu, Hagar Hsiao-Tien, Sun Yung-Chen, Lai Hsing-Lin, Kuo Min-Hao, Blum David, Buée Luc, Jin Lee-Way, Chen Shih-Yu, Ko Tai-Ming, Huang Jie-rong, Kuo Hung-Chih, Liu Fu-Tong, Chern Yijuang* Galectin-3 aggravates microglial activation and tau transmission in tauopathy Journal of Clinical Investigation e165523, 1 (2023-11-31) [JCR] [WOS]
- Lin Chia-Yeh, (Chang Yao-Ming), Tseng Hsin-Yi, Shih Yen-Ling, Yeh Hsiao-Hui, Liao You-Rou, Tang Han-Hsuan, Hsu Chia-Ling, Chen Chien-Chang, Yan Yu-Ting*, Kao Cheng-Fu* Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization Cell Reports 42(11), 113416 (2023-11-28) [JCR] [WOS]
- Chang Shao-Han, (Chang Yao-Ming), Chen Huan-Yuan, Shaw Fu-Zen, Shyu Bai-Chuang* Time-course analysis of frontal gene expression profiles in the rat model of posttraumatic stress disorder and a comparison with the conditioned fear model Neurobiology of Stress 2352-2895(23), 100569 (2023-09-20) [JCR] [WOS]
- Wei Ke-Hsuan, Lin I-Ting, Chowdhury Kaushik, Lim Khai Lone, Liu Kuan-Ting, Ko Tai-Ming, (Chang Yao-Ming), Yang Kai-Chien, Lai Shih-Lei* Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration eLife 12, e84679 (2023-07-27) [JCR] [WOS]
- Chuang Tzu-Wei, Su Chun-Hao, Wu Pei-Yu, (Chang Yao-Ming), Tarn Woan-Yuh* LncRNA HOTAIRM1 functions in DNA double-strand break repair via its association with DNA repair and mRNA surveillance factors Nucleic Acids Research gkad143, 1 (2023-03-07) [JCR] [WOS]
- Weng Yu-Ting, (Chang Yao-Ming), Chern Yijuang* The Impact of Dysregulated microRNA Biogenesis Machinery and microRNA Sorting on Neurodegenerative Diseases International Journal of Molecular Sciences 24(4), 3443 (2023-02-08) [JCR] [WOS]
- Hsiang-Chi Huang, Shih-Han Wang, Guo-Chen Fang, Wen-Cheng Chou, Chun-Che Liao, Cheng-Pu Sun, Jia-Tsrong Jan, Hsiu-Hua Ma, Hui-Ying Ko, Yi-An Ko, Ming-Tsai Chiang, Jian-Jong Liang, Chun-Tse Kuo, Te-An Lee, Diego Morales-Scheihing, Chen-Yang Shen, Shih-Yu Chen, Louise D. McCullough, Mi-Hua Tao, Yi-Ling Lin, (Yao-Ming Chang)*, Shu-Ping Wang*, Yun-Ju Lai*, and Chia-Wei Li* Upregulation of PD‐L1 by SARS‐CoV‐2 promotes immune evasion JOURNAL OF MEDICAL VIROLOGY 95(2), e28478 (2023-01-07) [JCR] [WOS]
- Yu-Shuen Tsai#, (Yao-Ming Chang)#, Yang-Mooi Lim, Soon-Keng Cheong, I-Fang Chung*, Chee-Yin Wong* Generating transcriptional regulatory networks from time-ordered stem cell differentiation RNA sequencing data STAR Protocols 3(3), 101541 (2022-09-16) [JCR] [WOS]