蔡松智博士
助研究員
- 02-2789-9163 (Lab)
- 02-2652-3934 (Office)
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Specialty:
Epitranscriptomic RNA modifications
Virus-host interactions
Education and Positions:
Ph.D., University of Pennsylvania, Perelman School of Medicine
Post Doctoral, Duke University Medical Center, Department of Molecular Genetics and Microbiology
近來研究進展發現核糖核酸(RNA)上的小分子修飾對於RNA的各種功能有十分重要的調控作用，這被稱為RNA表觀遺傳學 (epitranscriptomics)。RNA修飾在病毒的感染與複製過程中扮演著重要的角色。RNA中的腺嘌呤上的甲基化修飾 (m6A) 可增進人類免疫缺乏病毒 (HIV-1)、流感A病毒 (IAV) 等多種病毒的複製; 而RNA核糖鏈的甲基化 (Nm)和m6A甲基化皆可防止病毒的RNA被宿主細胞的先天免疫偵測到。此外，我們也發現胞嘧啶上的甲基化與乙醯化修飾(m5C & ac4C)也可以分別促進HIV-1病毒基因的轉譯與RNA之穩定。以上皆顯示 RNA修飾對病毒感染與複製的過程有著重要的影響。不過，以上多種RNA修飾對HIV-1以外之病毒的調控與機制還有許多未知之處。因次，本實驗室針對流感A病毒 (IAV) 、HIV-1與B肝病毒(HBV)去了解RNA修飾對這些病毒的感染與致病機制有何影響，進而提供未來對抗病毒的潛在新途徑。
Our Team
期刊 21
專書 1
期刊 21 專書 1
- (Tsai Kevin), Bogerd Hal P., Kennedy Edward M., Emery Ann, Swanstrom Ronald, Cullen Bryan R. Epitranscriptomic addition of m6A regulates HIV-1 RNA stability and alternative splicing GENES & DEVELOPMENT 35(13-14), 992-1004 (2021) [JCR] [WOS]
- Martinez Campos C, Tsai K, Courtney DG, Bogerd HP, Holley CL, Cullen BR Mapping of pseudouridine residues on viral and cellular transcripts using a novel antibody-based technique. RNA (New York, N.Y.) rna.078940.121, 121-121 (2021) [JCR] [WOS]
- Cullen Bryan R., (Tsai Kevin) Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification Sequencing Methods in Molecular Biology 123-134 (2021) [JCR] [WOS]
- (Tsai Kevin), Jaguva Vasudevan Ananda Ayyappan, Martinez Campos Cecilia, Emery Ann, Swanstrom Ronald, Cullen Bryan R. Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability Cell Host & Microbe 28(2), 306-312.e6 (2020) [JCR] [WOS]
- (Tsai Kevin), Cullen Bryan R. Epigenetic and epitranscriptomic regulation of viral replication Nature Reviews Microbiology 18(10), 559-570 (2020) [JCR] [WOS]
- Irwan Ishak D., Karnowski Heather L., Bogerd Hal P., (Tsai Kevin), Cullen Bryan R. Reversal of Epigenetic Silencing Allows Robust HIV-1 Replication in the Absence of Integrase Function mBio 11(3), e01038-20 (2020) [JCR] [WOS]
- Courtney David G., (Tsai Kevin), Bogerd Hal P., Kennedy Edward M., Law Brittany A., Emery Ann, Swanstrom Ronald, Holley Christopher L., Cullen Bryan R. Epitranscriptomic Addition of m5C to HIV-1 Transcripts Regulates Viral Gene Expression Cell Host & Microbe 26(2), 217-227.e6 (2019) [JCR] [WOS]
- (Tsai Kevin), Courtney David G., Cullen Bryan R. Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication PLOS Pathogens 14(2), e1006919 (2018) [JCR] [WOS]
- (Tsai K), Courtney DG, Kennedy EM, Cullen BR Influenza A virus-derived siRNAs increase in the absence of NS1 yet fail to inhibit virus replication. RNA (New York, N.Y.) 24(9), 1172-1182 (2018) [JCR] [WOS]
- Poling Brigid Chiyoko, (Tsai Kevin), Kang Dong, Ren Linda, Kennedy Edward M., Cullen Bryan R. A lentiviral vector bearing a reverse intron demonstrates superior expression of both proteins and microRNAs RNA Biology 14(11), 1570-1579 (2017) [JCR] [WOS]
