YH Tseng, HP Lin, SY Lin, BM Chen, TN Nguyen Vo, SH Yang, YC Lin, Z Prijovic, A Czosseck, YL Leu, SR Roffler*
Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrug
J Control Release., 369:179-198 (2024)
Chen WA, Chang DY, Chen BM, Lin YC, Barenholz Y*, Roffler SR*
Antibodies against poly(ethylene glycol) activate innate immune cells and induce hypersensitivity reactions to PEGylated nanomedicines
ACS Nano 17:5757-5772 (2023)
Trieu Tm Tran and Steve R Roffler*
Interactions between nanoparticle corona proteins and the immune system
Curr Opin Biotechnol, 84:103010 (2023)
Beishenaliev A, Loke YL, Goh SJ, Geo HN, Mugila M, Misran M, Chung LY, Kiew LV, Roffler S*, Teo YY*
Bispecific antibodies for targeted delivery of anti-cancer therapeutic agents: A review
J. Control. Release,359:268-286 (2023)
Lin YC, Chen BM, Tran TTM, Chang TC, Al-Qaisi TS, Roffler SR*
Accelerated clearance by antibodies against methoxy PEG depends on pegylation architecture
J Control Release., 354:354-367 (2023)
Y Bavli, BM Chen, G Gross, A Hershko, Keren Turjeman, S Roffler*, Y Barenholz*
Anti-PEG antibodies before and after a first dose of Comirnaty® (mRNA-LNP-based SARS-CoV-2 vaccine)
J. Control. Release, 354:316-322 (2023)
BK Sahoo, YC Lin, CF Tu, CC Lin, WJ Liao, FA Li, LH Li, KY Mou, SR Roffler, SP Wang, CT Yeh, CY Yao, HA Hou, WC Chou, HF Tien, RB Yang
Signal peptide-CUB-EGF-like repeat-containing protein 1-promoted FLT3 signaling is critical for the initiation and maintenance of MLL-rearranged acute leukemia
Haematologica 108:1284-1299 (2023)
YC Su, MT Nguyen, YC Shih, MH Lin, S Roffler, CY Hsiao, TL Cheng, WW Lin, EC Lin, YJ Jong and CY Chang
Structural determination of an antibody that specifically recognizes polyethylene glycol with a terminal methoxy group
Commun Chem. 2022; 5(1): 88
PA Burnouf, SR Roffler, CC Wu, SY Cheng
Glucuronides: From biological waste to bio-nanomedical applications
J. Control. Release, 349: 765-782 (2022)
Lin HJ, Liang TL, Chang YY, Liu DZ, Fan JY, Roffler SR, Lin SY
Development of Irinotecan Liposome Armed with Dual-Target Anti-Epidermal Growth Factor Receptor and Anti-Fibroblast Activation Protein-Specific Antibody for Pancreatic Cancer Treatment
Pharmaceutics, 14(6):1202 (2022)
Kim J, Li C, Wang H, Kaviraj S, Singh S, Savergave L, Raghuwanshi A, Gil S, Germond A, Baldessari A, Chen B, Roffler S, Fender P, Drescher C, Carter D, Lieber A
Translational development of a tumor junction opening technology
Sci Rep. 2022 12:7753 (2022)
Cheng WJ, Chuang KH, Lo YJ, Chen M, Chen YJ, Roffler SR, Ho HO, Lin SY, Sheu MT
Bispecific T-cell engagers non-covalently decorated drug-loaded PEGylated nanocarriers for cancer immunochemotherapy
J Control Release, doi: 10.1016/j.jconrel.2022.03.015 (2022)
KW Cheng, CH Tseng, IJ Chen, BC Huang, HJ Liu, KW Ho, WW Lin, CH Chuang, MY Huang, YL Leu, S Roffler, JY Wang, YL Chen, TL Cheng
Inhibition of gut microbial β-glucuronidase effectively prevents carcinogen-induced microbial dysbiosis and intestinal tumorigenesis
Pharmacol Res., doi: 10.1016/j.phrs.2022.106115 (2022)
H Wang, C Li, A Obadan, H Frizzell, TY Hsiang, S Gil, A Germond, C Fountain, A Baldessari, S Roffler, HP Kiem, D Fuller, A Lieber
In vivo HSC gene therapy for SARS-CoV2 infection using a decoy receptor
Hum Gene Ther., doi: 10.1089/hum.2021.295 (2022)
MA Al-Aghbar, A Jainarayanan, ML Dustin, SR Roffler
Interplay between membrane topology and mechanical forces in TCR triggering and regulation of T cell activity
Commun Biol., 5:40. doi: 10.1038/s42003-021-02995-1 (2022)
TC Chang, BM Chen, JY Wu, TL Cheng, SR Roffler.
Impact of anti-PEG antibody affinity on accelerated blood clearance of pegylated epoetin beta in mice
Biomed. Pharmacother. 146:112502. doi: 10.1016/j.biopha.2021.112502 (2021)
Chen BM, Cheng TL, and Roffler SR
Polyethylene Glycol Immunogenicity: Theoretical, Clinical, and Practical Aspects of Anti-Polyethylene Glycol Antibodies
ACS Nano doi.org/10.1021/acsnano.1c05922 (2021)
Lin HY, Chen CY, Lin TC, Yeh LF, Hsieh WC, Gao S, Burnouf PA, Chen BM, Hsieh TJ, Dashnyam P, Kuo YH, Tu Z, Roffler SR andLin CH
Entropy-driven binding of gut bacterial beta-glucuronidase inhibitors ameliorates irinotecan-induced toxicity
Commun. Biol. doi: 10.1038/s42003-021-01815-w (2021)
Sylvestre M, Lv S, Yang LF, Luera N, Peeler DJ, Chen BM, Roffler SR, Pun SH.
Replacement of L-amino acid peptides with D-amino acid peptides mitigates anti-PEG antibody generation against polymer-peptide conjugates in mice
J Control Release. doi: 10.1016/j.jconrel.2021.01.015 (2021)
Ho KW, Chen IU, Cheng YA, Liao TY, Liu ES, Chen HJ, Lu YC, Su YC, Roffler SR, Huang BC, Liu HJ, Huang MY, Chen CY, Cheng TL
Double attack strategy for leukemia using a pre-targeting bispecific antibody (CD20 Ab-mPEG scFv) and actively attracting PEGylated liposomal doxorubicin to enhance anti-tumor activity
J Nanobiotechnology 19(1):16. doi: 10.1186/s12951-020-00752-w (2021)
Fang JL, Beland FA, Tang Y, Roffler SR
Flow cytometry analysis of anti-polyethylene glycol antibodies in human plasma
Toxicol Rep. 8:148-154 (2021)
Chen E, Chen BM, Su YC, Chang YC, Cheng TL, Barenholz Y, Roffler SR
Premature drug release from polyethylene glycol (PEG)-coated liposomal doxorubicin via formation of the membrane attack complex
ACS Nano. 14: 7808-7822 (2020)
IJ Chen, YA Cheng, KW Ho, WW Lin, KW Cheng, YC Lu, YC Hsieh, CC Huang, CH Chuang, FM Chen, YC Su, SR Roffler, TL Cheng
Bispecific Antibody (HER2×mPEG) Enhances Anti-Cancer Effects by Precise Targeting and Accumulation of mPEGylated Liposomes
Acta Biomaterialia 111:386-397 (2020)
Bavli Y, Chen BM, Roffler SR, Dobrovolskaia MA, Elnekave E, Ash S, Barenholz Y, Turjeman K
PEGylated liposomal methyl prednisolone succinate does not induce infusion reactions in patients: A correlation between in vitro immunological and in vivo clinical studies
Molecules. 25 doi: 10.3390/molecules25030558 (2020)
Lee CC, Su YC, Ko TP, Lin LL, Yang CY, Chang SS, Roffler SR, Wang AH
Structural basis of polyethylene glycol recognition by antibody
J Biomed Sci. doi: 10.1186/s12929-019-0589-7 (2020)
Chang TC, Chen BM, Lin WW, Yu PH, Chiu YW, Chen YT, Wu JY, Cheng TL, Hwang DY, Roffler SR
Both IgM and IgG antibodies against polyethylene glycol can alter the biological activity of methoxy polyethylene glycol-epoetin beta in mice
Pharmaceutics doi: 10.3390/pharmaceutics12010015 (2019)
Cheng YA, Chen IJ, Su YC, Cheng KW, Lu YC, Lin WW, Hsieh YC, Kao CH, Chen FM, Roffler SR, Cheng TL
Enhanced drug internalization and therapeutic efficacy of PEGylated nanoparticles by one-step formulation with anti-mPEG bispecific antibody in intrinsic drug-resistant breast cancer
Biomater Sci. 7:3404-3417 (2019)
Bavli Y, Winkler I, Chen BM, Roffler S, Cohen R, Szebeni J, Barenholz Y.
Doxebo (doxorubicin-free Doxil-like liposomes) is safe to use as a pre-treatment to prevent infusion reactions to PEGylated nanodrugs
J Control Release 306:138-148 (2019)
Chen YJ, Chen M, Cheng TL, Roffler SR, Lin SY, Hsu HL, Wang CH, Chen CY, Kao AP, Cheng JJ, Chuang KH
Simply mixing poly protein G with detection antibodies enhances the detection limit and sensitivity of immunoassays.
Anal Chem. doi: 10.1021/ acs.analchem.9b01077 (2019)
MA Al-Aghbar, YS Chu, BM Chen, and SR Roffler
High-Affinity Ligands Can Trigger T Cell Receptor Signaling Without CD45 Segregation
Front. Immunol., 10.3389 (2018)
TS Al-Qaisi, YC Su, SR Roffler
Transient AID expression for in situ mutagenesis with improved cellular fitness
Scientific Reports. 8:9413 (2018)
YC Hsieh, HE Wang, WW Lin, SR Roffler, TC Cheng, YC Su, Jia-Je Li, CC Chen, CH Huang, BM Chen, JY Wang, TL Cheng, Fang-Ming Chen
Pre-existing anti-polyethylene glycol antibody reduces the therapeutic efficacy and pharmacokinetics of PEGylated liposomes
Theranostics 8:3164-3175 (2018)
PA Burnouf, YL Leu, YC Su, K Wu, W.C. Lin and SR Roffler
Reversible attachment of a glycosidic switch to drugs for secure delivery in nanoliposomes
Nat. Comm. 9(1):1843 (2018)
YC Hsieh, HE Wang, WW Lin, SR Roffler, TC Cheng, YC Su, Jia-Je Li, CC Chen, CH Huang, BM Chen, JY Wang, TL Cheng, Fang-Ming Chen
Pre-existing anti-polyethylene glycol antibody reduces the therapeutic efficacy and pharmacokinetics of PEGylated liposomes
Theranostics 8:3164-3175 (2018)
Chang CJ, Chen CH, Chen BM, Su YC, Chen YT, Hershfield MS, Lee MM, Cheng TL, Chen YT, Roffler SR, Wu JY
A genome-wide association study identifies a novel susceptibility locus for the immunogenicity of polyethylene glycol
Nat Commun. 8(1):522 (2017)
YC Su, PA Burnouf, KH Chuang, BM Chen, TL Cheng and SR Roffler
Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
Nat. Comm., 8:15507 (2017)
BM Chen, MA Al-Aghbar, CH Lee, TC Chang, YC Su, YC Li, SE Chang, CC Chen, TH Chung, YJ Liao, CH Lee and SR Roffler
The Affinity of Elongated Membrane-Tethered Ligands Determines Potency of T Cell Receptor Triggering
Front. Immunol. 8:793 (2017)
WW Lin, YA Cheng, CH Kao, SR Roffler, YC Lee YC, BM Chen, YC Hsieh, IJ Chen, BC Huang, YT Wang, YC Tung, MY Huang, FM Chen, TL Cheng
Enhancement effect of a variable topology of a membrane-tethered anti-poly(ethylene glycol) antibody on the sensitivity for quantifying PEG and PEGylated molecules
Anal. Chem., 89: 6082-6090 (2017)
Lin YC, Chao TY, Yeh CT, Roffler SR, Kannagi R, Yang RB
Endothelial SCUBE2 interacts With VEGFR2 and regulates VEGF-induced angiogenesis
Arterioscler Thromb Vasc Biol. 37:144-155 (2017)
Wu JP, Cheng B, Roffler SR, Lundy DJ, Yen CY, Chen P, Lai JJ, Pun SH, Stayton PS, Hsieh PC
Reloadable multidrug capturing delivery system for targeted ischemic disease treatment
Sci Transl Med. 8(365):365ra160, 2016
YC Hsieh, TC Cheng, HE Wang, JJ Li, WW Lin, CC Huang, CH Chuang, YT Wang, JY Wang, SR Roffler, KH Chuang, TL Cheng.
Using anti-poly(ethylene glycol) bioparticles for the quantitation of PEGylated nanoparticles
Sci. Rep. 6:39119 (2016)
WW Lin, YC Hsieh, YA Cheng, KH Chuang, CC Huang, CH Chuang, IJ Chen, KW Cheng, YC Lu, TC Cheng, YT Wang, SR Roffler and TL Cheng
Optimization of an anti-poly(ethylene glycol) (anti-PEG) cell-based capture system to quantify PEG and PEGylated molecules
Anal. Chem. 88:12371–12379 (2016)
Chen BM, Su YC, Chang CJ, Burnouf PA, Chuang KH, Chen CH, Cheng TL, Chen YT, Wu JY, Roffler SR
Measurement of pre-existing IgG and IgM antibodies against polyethylene glycol in healthy individuals
Anal Chem. 88:10661-10666, 2016
WW Lin, IJ Chen, TC Cheng, YC Tung, PY Chu, CH Chuang, YC Hsieh, CC Huang, YT Wang, CH Kao, SR Roffler and TL Cheng
A secondary antibody-detecting molecular weight marker with mouse and rabbit IgG Fc linear epitopes for western blot analysis
PloS One 11(8):e0160418, 2016
Henry CE, Wang YY, Yang Q, Hoang T, Chattopadhyay S, Hoen T, Ensign LM, Nunn KL, Schroeder H, McCallen J, Moench T, Cone R, Roffler SR, Lai SK
Anti-PEG antibodies alter the mobility and biodistribution of densely PEGylated nanoparticles in mucus
Acta Biomater. 2016
Lin SI, Huang MH, Chang YW, Chen IH, Roffler S, Chen BM, Sher YP, Liu SJ
Chimeric peptide containing both B and T cells epitope of tumor-associated antigen L6 enhances anti-tumor effects in HLA-A2 transgenic mice
Cancer Lett. 28: 126-133, 2016
WC Huang, PA Burnouf, YC Su, BM Chen, KH Chuang, CW Lee, PK Wei, TL Cheng and SR Roffler
Engineering chimeric receptors to investigate the size and rigidity-dependent interaction of PEGylated nanoparticles with cells
ACS Nano 10:648-662, 2016
ZM Prijovich, PA Burnouf, PT Huang, KC Chen, TL Cheng, YL Leu and SR Roffler
Synthesis and antitumor properties of BQC-glucuronide, a camptothecin prodrug for selective tumor activation
Mol. Pharm. 13: 1242-1250, 2016
Kooijmans SA, Aleza CG, Roffler SR, van Solinge WW, Vader P, Schiffelers RM
Display of GPI-anchored anti-EGFR nanobodies on extracellular vesicles promotes tumour cell targeting
J Extracell Vesicles 5:31053. doi: 10.3402/jev.v5.31053, 2016
Sher YP, Lin SI, Chen IH, Liu HY, Lin CY, Chiang IP, Roffler S, Chen HW, Liu SJ.
A HLA-A2-restricted CTL epitope induces anti-tumor effects against human lung cancer in mouse xenograft model
Oncotarget 7:671-683, 2016
YT Hsieh, PT Huang, ZM Projovich and SR Roffler
Effect of cellular location of human carboxylesterase 2 on CPT-11 hydrolysis and antitumor activity
PLoS One 10:e0141088, 2015.
HY Chuang, CS Suen, MJ Hwang and SR Roffler
Toward reducing immunogenicity of enzyme replacement therapy: altering the specificity of human beta-glucuronidase to compensate for alpha-iduronidase deficiency
Protein Eng. Des. Sel. 28:519-30, 2015
HY Tung, YC Su, BM Chen, PA Burnouf, WC Huang, KH Chuang,YT Yan, TL Cheng and SR Roffler
Selective delivery of PEGylated compounds to tumor cells by anti-PEG hybrid antibodies
Mol. Cancer Ther. 14:1317-26, 2015.
M Richter, R Yumul, H Wang, K Saydaminova, M Ho, D May, A Baldessari, M Gough, C Drescher, N Urban, S Roffler, C Zubieta, D Carter, P Fender, A Lieber
Preclinical safety and efficacy studies with an affinity-enhanced epithelial junction opener and PEGylated liposomal doxorubicin
Molecular Therapy - Methods & Clinical Development. Doi:10.1038/mtm.2015.5
Cheng TC, Chuang KH, Roffler SR, Cheng KW, Leu YL, Chuang CH, Huang CC, Kao CH, Hsieh YC, Chang LS, Cheng TL, Chen CS.
Discovery of specific inhibitors for intestinal E. coli beta-glucuronidase through in silico virtual screening
Scientific World Journal. 2015 doi: 10.1155/2015/740815
Mousavi MZ, Chen HY, Hou HS, Chang CY, Roffler S, Wei PK, Cheng JY
Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance
Biosensors (Basel). 5:98-117, 2015.
Hsieh YT, Chen KC, Cheng CM, Cheng TL, Tao MH, Roffler SR
Impediments to enhancement of CPT-11 anticancer activity by e. coli directed beta-glucuronidase therapy
PLoS One 2015 Feb 17;10(2):e0118028
Lin YC, Roffler SR, Yan YT, Yang RB
Disruption of Scube2 impairs endochondral bone formation
Bone Miner Res. 2015 Jan 12. doi: 10.1002/jbmr.2451
YC Su, TC Cheng, YL Leu, SR Roffler, JY Wang, CH Chuang, CH Kao, KC Chen, HE Wang, TL Cheng
PET imaging of beta-glucuronidase activity by an activity-based 124I-trapping probe for the personalized glucuronide prodrug targeted therapy
Mol Cancer Ther, 13: 2852-2863, 2014
CH Kao, JY Wang, KH Chuang, CH Chuang, TC Cheng, YC Hsieh, YL Tseng, BM Chen, SR Roffler, TL Cheng
One-step mixing with humanized anti-mPEG bispecific antibody enhances tumor accumulation and therapeutic efficacy of mPEGylated nanoparticles
Biomaterials 35: 9930-40, 2014
KH Chuang, CH Kao, SR Roffler, SJ Lu, TC Cheng, YM Wang, CH Chuang, YC Hsieh, YT Wang, JY Wang, KY Weng, and TL Cheng
Development of an anti-methoxy poly(ethylene glycol) (α-mPEG) cell-based capture system to measure mPEG and mPEGylated molecules.
Macromolecules 47: 6880-6888, 2014
KH Chuang, YC Hsieh, IS Chiang, CH Chuang, CH Kao, TC Cheng, YT Wang, WV Lin, BM Chen, SR Roffler, MY Huang, TL Cheng
High-throughput sorting of the highest producing cell via a transiently protein-anchored system
PLoS One 2014 9:e102569
YC Su, TS Al-Qaisi, HY Tung, TL Cheng, KH Chuang, BM Chen, and SR Roffler
Mimicking the germinal center reaction in hybridoma cells to isolate temperature-selective anti-PEG antibodies
Mabs 6: 1069-83, 2014
MY Wu, YC Lin, WJ Liao, CF Tu, MH Chen, SR Roffler and RB Yang
Inhibition of the plasma SCUBE1, a movel pletelet adhesive protein, protects mice against thrombosis
Arteriosclerosis, Thrombosis, and Vascular Biology (ePub 2014)
CY Lai, PR Wu, SR Roffler, ST Lee, SM Hwang, SS Wang, K Wang, PC Hsieh
The clearance kinetics of biomaterials affects stem cell retention and therapeutic efficacy
Biomacromolecules 15:564-73, 2014
ZM Prijovich, PA Burnouf and SR Roffler
Versatile on-line SPE HPLC method for analysis of Irinotecan and its clinically relevant metabolites in biomaterials
J. Separation Science 37:360-367, 2014
YC Lin, WC Lu, CI Su, ZM Prijovich, BM Chen, WC Chung, PY Wu, KC Chen, IC Lee, TY Juan and SR Roffler
The B7-1 cytoplasmic tail enhances intracellular transport and mammalian cell surface display of chimeric proteins in the absence of a linear ER export motif
PLoS One 8(9):e75084, 2013
TC Cheng, KH Chuang, M Chen, HE Wang, SC Tzou, YC Su, CH Chuang, CH Kao, BM Chen, LS Chang, SR Roffler, and TL Cheng
Sensitivity of PEGylated interferon detection by anti-polyethylene glycol (PEG) antibodies depends on PEG length
Bioconjugate Chem., 24: 1408-1413, 2013
CM Cheng, FM Chen, YL Lue, SC Tzou, JY Wang, CH Kao, KW Liao, TC Cheng, CH Chuang, BM Chen, SR Roffler and TL Cheng
Expression of beta-glucuronidase on the surface of bacteria enhances activation of glucuronide prodrugs
Cancer Gene Ther., 20: 276-281, 2013
DV Chan, R Sharma, CY Ju, SR Roffler and ST Ju
A recombinant scFv-FasLext as a targeting cytotoxic agent against human Jurkat-Ras cancer
J. Biomed. Sci., 20: 16, 2013
KC Chen, K Schmuck, LF Tietze and SR Roffler
Selective cancer therapy by extracellular activation of a highly potent glycosidic duocarmycin analog.
Mol. Pharm., 10:1773-1782, 2013
CH Kao, CM Cheng, KH Chuang, CH Chuang, SC Tzou,TC Cheng, YC Hsieh, KW Liao, YM Wang, LS Chang, SR Roffler, FM Chen, TL Cheng
A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis
Anal. Biochem., 431: 1-3, 2012
TL Cheng, KH Chuan, BM Chen and SR Roffler
Analytical measurement of PEGylated molecules
Bioconjugate Chem., 23: 881-899, 2012
CP Chen, YT Hsieh, ZM Prijovich, HY Chuang, KC Chen, WC Lu, Q Tseng, YL Leu, TL Cheng, SR Roffler
ECSTASY, an adjustable membrane-tethered/soluble protein expression system for the directed evolution of mammalian proteins
Protein Eng Des Sel., 25:367-375, 2012
I Beyer, H Cao, J Persson, H Song, M Richter, Q Feng, R Yumul, R van Rensburg, Z Li, R Berenson, D Carter, S Roffler, C Drescher, A Lieber
Coadministration of epithelial junction opener JO-1 improves the efficacy and safety of chemotherapeutic drugs
Clin. Cancer Res., 18:3340-3351, 2012
Pan WY, Lo CH, Chen CC, Wu PY, Roffler SR, Shyue SK, Tao MH
Cancer immunotherapy using a membrane-bound interleukin-12 with B7-1 transmembrane and cytoplasmic domains
Mol Ther., 20:927-937,2012
CH Chuang, KH Chuang, HE Wang, SR Roffler, SC Tzou, TC Cheng, CH Kao, SY Wu, WL Tseng, J Shiea, JM Wang and TL Cheng
In vivo positron emission tomography imaging of protease activity by generation of a hydrophobic product from a noninhibitory protease substrate
Clin Cancer Res., 18:238-247, 2012
SH Tu , HI Huang, SI Lin, HY Liu, YP Sher, SK Chiang, P Chong, S Roffler, HW Chen, SJ Liu
A Novel HLA-A2-restricted CTL epitope of tumor-associated antigen L6 can inhibit tumor growth in vivo
J. Immunother., 35: 235-244, 2012
TC Cheng, SR Roffler, SC Tzou, KH Chuang, YC Su, CH Chuang, CS Chen, IH Harn, KY Liu, TL Cheng and YL Leu
An activity-based near infrared beta-glucuronide trapping probe for imaging beta-glucuronidase expression in deep tissues
J. Am. Chem. Soc., 132:3103-3110, 2012
J Persson, I Beyer, R Yumul, Z Ki, HP Kiem, S Roffler and A Lieber
Immuno-therapy with anti-CTLA4 antibodies in tolerized and non-tolerized mouse tumor models
PLoS One 6:e22303, 2011
KC Chen, SY Wu, YL Leu, ZM Orijovich, BM Chen, HE Wang, TL Cheng and SR Roffler
A humanized immunoenzyme with enhanced activity for glucuronide prodrug activation in the tumor microenvironment
Bioconjugate Chem., 22: 938-948, 2011
Y Liu, S Tuve, J Persson, I Beyer, R Yumel, ZY Li, K Tragoolpua, KE Hellstrom, S Roffler and A Lieber
Adenovirus-mediated intratumoral expression of immunostimulatory proteins in combination with systemic Treg inactivation induces tumor-destructive immune responses in mouse models
Cancer Gene Ther., 18: 407-418, 2011
PT Huang, KC Chen, ZM Prijovich, TL Cheng, YL Leu, SR Roffler
Enhancement of CPT-11 antitumor activity by adenovirus-mediated expression of beta-glucuronidase in tumors
Cancer Gene Ther., 18: 381-389, 2011
YC Su, BM Chen, KH Chuang, TL Cheng and SR Roffler
Sensitive quantitation of pegylated proteins and nanoparticles by second generation anti-polyethylene glycol monoclonal antibodies
Bioconjugate Chem., 21: 1264-1267, 2010
KH Chuang, HE Wang, TC Cheng, SC Tzou, CM Cheng, CH Kao, CH Chuang, WL Tseng, WC Hung, SR Lin, BM Chen, SR Roffler and TL Cheng
Development of an universal anti-polyethylene glycol reporter gene for non-invasive imaging of PEGylated probes
J. Nucl. Med., 51: 933-941, 2010
YC Li,BM Chen, PC Wu, TL Cheng, LS Kao, MH Tao, A Lieber and SR Roffler
Mechanical forces acting on T cells immobilized via the TCR complex can trigger TCR signaling
J. Immunol. (Cutting Edge) 184: 5959-5963, 2010
KH Chuang, HE Wang, FM Chen, SC Tzou, CM Cheng,YC Chang, WL Tseng, J Shiea, SR Lin, JY Wang, BM Chen, SR Roffler and TL Cheng
Endocytosis of PEGylated agents enhances cancer imaging and anti-cancer efficacy
Mol. Cancer Ther., 9: 1903-1912, 2010
Chuang KH, Tzou SC, Cheng TC, Kao CH, Tseng WL, Shiea J, Liao KW, Wang YM, Chang YC, Huang BJ, Wu CJ, Chu PY, Roffler SR and Cheng TL
Measurement of poly(ethylene glycol) by cell-based anti-poly(ethylene glycol) ELISA
Anal Chem., 82:2355-62, 2010
Chang CM, Lo CH, Shih YM, Chen Y, Wu PY, Tsuneyama K, Roffler S, Tao MH.
Treatment of metastatic hepatocellular carcinoma by adeno-associated virus encoding interleukin-15 superagonist
Hum Gene Ther., 21: 611-621, 2010
Jung T, Castellana D, Klingbeil P, Hernadez IC, Vitacolonna M, Orlicky DJ, Roffler SR, Brodt P, Zoler M.
CD44v6 dependence of premetastatic niche preparation by exosomes
Neoplasia 11:1093-105, 2009
TY Juan, SR Roffler, HS Hou, SM Huang, KC Chen, YL Leu, ZM Prijovich, CP Yu, CC Wu, GH Sun and TL Cheng
Antiangiogenesis targeting tumor microenvironment synergizes glucuronide prodrug antitumor activity
Clinical Cancer Res., 15:4600-4611, 2009
SC Tzou, S Roffler, KH Chuang, HP Yeh, CH Kao, YC Su, CM Cheng, WL Tseng, J Shiea, KW Cheng, BM Chen, TL Cheng and HE Wang
Micro-PET imaging of beta-glucuronidase activity by the hydrophobic conversion of a glucuronide probe
Radiology, 252: 754-762, 2009
SJ Shieh, FC Chou, PN Yu, WC Lin, DM Chang, SR Roffler, HK Sytwu
Transgenic expression of single-chain anti-CTLA-4 Fv on beta cells protects nonobese diabetic mice from autoimmune diabetes
J Immunol. 183: 2277-85, 2009
S Tuve, Y Liu, K. Tragoolpua, JD Jacobs, RC Yumul, ZY Li, R Strauss, KE Hellstrom, ML Disis, S Roffler and A Lieber
In situ adenovirus vaccination engages T effector cells against cancer
Vaccine 27: 4225-4239, 2009
ZM Prijovich, KC Chen, SR Roffler
Local enzymatic hydrolysis of an endogenously-generated metabolite can enhance CPT-11 anti-cancer efficacy
Mol Cancer Ther., 8: 940-946, 2009
CH Lee, YH Chiang, SE Chang, CL Chong, BM Cheng and SR Roffler
Tumor-localized ligation of CD3 and CD28 with systemic regulatory T cell depletion induces potent innate and adaptive antitumor responses
Clin Cancer Res., 15: 2756-2766, 2009
CM Cheng, PY Chu, KH Chuang, SR Roffler, CH Kao, WL Tseng, J Shiea, WD Chang, YC Su, BM Chen, YM Wang and TL Cheng
Hapten-derivatized nanoparticle targeting and imaging of gene expression by multimodality imaging systems
Cancer Gene Ther., 16: 83-90, 2009
KC Chen, CH Wu, CY Chang, WC Lu, Q Tseng, ZM Prijovich, W Schechinger, YC Liaw, YL Leu and SR Roffler
Directed evolution of a lysosomal enzyme with enhanced activity at neutral pH by mammalian cell-surface display
Chem. Biol. 15: 1277-86, 2008
H Wang, Y Liu, Z Li, S Tuve, D Stone, O Kalyushniy, D Shayakhmetov, CL Verlinde, T Stehle, J McVey, A Baker, KW Peng, S Roffler, A Lieber
In vitro and in vivo properties of adenovirus vectors with increased affinity to CD46
J Virol. 82: 10567-79, 2008
TL Cheng and SR Roffler
Membrane-tethered proteins for basic research, imaging and therapy
Med Res Rev., 28: 885-928, 2008
Cheng, YL Lu, CH Chuang, WC Hung, J Shiea, YC Su, CH Kao, BM Chen, S Roffler, TL Cheng
Tumor-targeting prodrug-activating bacteria for cancer therapy
Cancer Gene Ther., 15: 393-401, 2008
H Wang, YC Liaw, D Stone, O Kalyuzhniy, I Amiraslanov, S Tuve, CL Verlinde, D Shayakhmetov, T Stehle, S Roffler and A Lieber
Identification of CD46 binding sites within the adenovirus Serotype 35 fiber knob
J. Virol., 81:12785-92, 2007
S Tuve, BM Chen, Y Liu, TL Cheng, P Toure, PS Sow, Q Feng, N Kiviat, S Ni, ZY Li, SR Roffler and A Lieber
Combination of tumor-site located CTLA-4 blockade and systemic regulatory T cell depletion induces anti-tumor immunity but not autoimmunity
Cancer Res., 67:5929-39, 2007
P Volna, J Jarjour, S Baxter, RJ Monnat, BL Stoddard, SR Roffler and AM Scharenberg
Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases
Nucleic Acids Res., 35:2748-58, 2007
KC Chen, TL Cheng, YL Leu, ZM Prijovich, CH Chuang, BM Chen and SR Roffler
Membrane-localized activation of glucuronide prodrugs by beta-glucuronidase enzymes
Cancer Gene Ther., 14: 187-200, 2007
ZM Prijovich, YL Leu and SR Roffler
Effect of pH and human serum albumin on the cytotoxicity of a glucuronide prodrug of 9-aminocamptothecin
Cancer Chemother Pharmacol., 60: 7-17, 2007
KH Chuang, CM Cheng, SR Roffler, YL Lu, SR Lin, JY Wang, WS Tzou, YC Su, BM Chen and TL Cheng
Combination cancer therapy by hapten-targeted prodrug-activating enzymes and cytokines
Bioconjugate Chem., 17: 707-714, 2006
D Kiewlich, J Zhang, C Gross, W Xia, B Larsen, RR Cobb, S Biroc, JM Gu, T Sato, DR Light, T Heitner, J Willuda, D Vogel, F Monteclaro, A Citkowicz, SR Roffler, DA Zajchowski
Anti-EphA2 antibodies decrease EphA2 protein levels in murine CT26 colorectal and human MDA-231 breast tumors but do not inhibit tumor growth
Neoplasia 8: 18-30, 2006
AB Liu and SR Roffler
Anti-CTLA4 scFv, a single-chain antibody, protects the expression of E. coli beta-galactosidase during repeated intramuscular injections in immune-competent mice
Tzu Chi Med J., 18: 259-265, 2006
SR Roffler, HE Wang, HM Yu, WD Chang,CM Cheng, YL Lu, BM Chen and TL Cheng
A membrane antibody receptor for noninvasive imaging of gene expression
Gene Ther. 13:412-20, 2006
MH Yu, SR Roffler and BM Chen
Topoisomerase I activity in ovarian cancer is a predictor of sensitivity to topotecan chemotherapy ?an exciting preliminary finding
Taiwan Gynecol. Oncol., 2:14-22, 2005
TL Cheng, CM Cheng, BM Chen, DA Tsao, KH Chuang, SW Hsiao, YH Lin and SR Roffler
Monoclonal antibody-based quantitation of poly(ethylene glycol)-derivatized proteins, liposomes, and nanoparticles
Bioconj. Chem. 16:1225-31, 2005
YW Chang, SC Chen, EC Cheng, YP Ko, YR Kao, YC Tsai, SY Chen, YG Tsay, PC Yang, CW Wu and SR Roffler
CD13 (aminopeptidase N) can associate with tumor-associated antigen L6 and enhance the motility of human lung cancer cells
Int. J Cancer 116:243-52, 2005
YP Sher, JY Shih, PC Yang, SR Roffler, YW Chu, CW Wu, CL Yu and K. Peck
Prognosis of non-small cell lung cancer patients by detecting circulating cancer cells in the peripheral blood with multiple marker genes
J Clin Oncol., 11:173-9, 2005
CJ Chang, KF Tai, SR Roffler and LH Hwang
The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors
J Immunol. 173:6025-32, 2004
TL Cheng, KW Liao, SC Tzou, CM Cheng, BM Chen, SR Roffler
Hapten-directed targeting to single-chain antibody receptors
Cancer Gene Therapy, 11: 380-8, 2004
AB Liu and SR Roffler
Anti-CTLA4 scFv expressed on cells can prolong the expression of Escherichia. coli. beta-galactosidase in immune-competent mice
Tzu Chi Med J. 16: 25-31, 2004
FH Chang, CH Lee, MT Chen, CC Kuo, YL Chiang, CY Hang and SR Roffler
Surfection: a new platform for transfected cell arrays
Nucleic Acids Res., 32:e33, 2004
CH Wu, WR Balasubramanian, YP Ko, G Hsu, SE Chang, ZM Prijovich, YC Chao and SR Roffler
Simple mammalian cell production of recombinant proteins
Biotechnol. Appl. Biochem., 40: 167-172, 2004
HK Sytwu, WD Lin, SR Roffler, JT Hung, HS Sung, CH Wang, TL Cheng, SC Tsou, SC Hsi, KL She
Anti-4-1BB-based immunotherapy for autoimmune diabetes: Lessons from a transgenic non-obese diabetic (NOD) model
J. Autoimmunity. 21: 247-54, 2003
ZM Prijovich, YL Leu and SR Roffler
Stability of the new prodrug 9-aminocamptothecin glucuronide (9ACG) in the presence of human serum albumin
Biochem. Pharmacol., 66: 1181-1187, 2003
KW Liao, BM Chen, TB Liu, SC Tzo, YM Lin, KF Lin, CI Su and SR Roffle
Stable expression of chimeric anti-CD3 receptors on mammalian cells for stimulation of anti-tumor immunity
Cancer Gene Therapy. 10: 779-790, 2003
CH Lo, SC Lee, PY Wu, WY Pan, J Su, CW Cheng, SR Roffler, BL Chiang, CN Lee, CW Wu and MH Tao
Antitumor and antimetastatic activity of IL-23
J. Immunol. 171: 600-607, 2003
YR Kao, JY Shih, WC Wen, YP Ko, BM Chen, YL Chan, YW Chu, PC Yang, CW Wu and SR Roffler
Tumor-associated antigen L6 (TAL6) and the invasion of human lung cancer cells
Clin. Cancer Res., 9: 2807-2816, 2003
NM Tsai, BM Chen, SL Wei, CW Wu and SR Roffler
Antitumor immunoglobulin M increases lung metastasis in an experimental model of malignant melanoma
Clin. Exp. Metastasis, 20: 103-109, 2003
KM Bernt, DS Steinwaerder, S Ni, ZY Li, SR Roffler and A Lieber
Enzyme-activated prodrug therapy enhances tumor-specific replication of adenovirus vectors
Cancer Res., 62: 6089-98, 2002
ZM Prijovich, BM Chen, YL Leu, JW Chern and SR Roffler
Antitumor activity and toxicity of the new glucuronide prodrug 9-aminocamptothecin glucuronide (9ACG) in mice
Br. J. Cancer, 86: 1634-1638, 2002
BM Chen, TL Cheng, SC Tzou and SR Roffler
Potentiation of antitumor immunity by antibody-directed enzyme prodrug therapy
Int. J. Cancer, 94: 850-858, 2001
JW Chen, K Peck, TM Hong, SC Yang, YP Sher, JY Shih, R Wu, SR Roffler, CW Wu and PC Yang
Global analysis of gene expression in invasion by a lung cancer model
Cancer Res., 61: 5223-5230, 2001
KW Liao, WC Chou, YC Lo and SR Roffler
Design of transgenes for efficient expression of active chimeric Proteins on mammalian cells
Biotechnol Bioeng, 73:313-323, 2001
NM Tsai, TL Cheng and SR Roffler
Sensitive quantitation of poly(ethylene glycol)-modified proteins
Biotechniques 30: 396-402, 2001
BM Chen, JY Chen, M Kao, JB Lin, MH Yu and SR Roffler
Elevated topoisomerase I activity in gynecological cancers as a target for chemoradiation therapy
Gynecoligic Oncol. 79: 272-280, 2000
Cheng TL, Chen BM, Chern JW, Wu MF and Roffler S
Efficient clearance of poly(ethylene glycol)-modified immunoenzyme with anti-PEG monoclonal antibody for prodrug cancer therapy
Bioconj. Chem., 11: 258-266, 2000
Hou CL, Tang CC, Roffler SR and Tang TK
Protein 4.1R binding to eIF3-p44 suggests an interaction between the cytoskeletal network and the translation apparatus
Blood, 96: 747-753, 2000
Liao KW, Lo YC, and Roffler SR
Activation of lymphocytes by anti-CD3 single-chain antibody dimers expressed on the plasma membrane of tumor cells
Gene Therapy 7: 339-347, 2000
Leu YL, Roffler SR and Chern JW
Design and synthesis of water-soluble glucuronide analogs of camptothecin for cancer prodrug monotherapy and antibody-directed enzyme prodrug therapy (ADEPT)
J. Med. Chem., 42: 3623-3628, 1999
Lomasney JW, Cheng HF, Roffler SR and King K
Activation of PLC δ1 by Ca-enzyme-phosphatidylserine ternary complex
J. Biol. Chem., 274: 21995-22001, 1999
Chou WC, Liao KW, Lo YC, Jiang SY, Yeh MY and Roffler SR
Expression of chimeric monomer and dimer proteins on the plasma membrane of mammalian cells
Biotechnol. Bioeng., 65: 160-169, 1999
Cheng TL, Chou WC, Chen BM, Chern JW and Roffler SR
Characterization of an antineoplastic glucuronide prodrug
Biochem. Pharmacol., 58: 325-328, 1999
Cheng TL, Wu PY, Wu MF, Chern JW, and Roffler SR
Accelerated clearance of polyethylene glycol-modified proteins by anti-polyethylene glycol IgM
Bioconj. Chem. 10: 520-528, 1999
Cheng TL, Wei SL, Chou WC, Chen BM, Wu MF, Liu PW, Chern JW, and Roffler SR
Bystander killing of tumor cells by antibody-targeted enzymatic activation of a glucuronide prodrug
Br. J. Cancer 79: 1378-1385. 1999
Shen AY, Chen CP, Roffler SR
A chelating agent possessing cytotoxicity and antimicrobial activity: 7-morpholinomethyl-8-hydroxyquinoline
Life Sciences 64: 813-825, 1999
Peck K, Sher YP, Shih JY, Roffler SR, Wu CW, and Yang PC
Detection and quantitation of circulating cancer cells in the peripheral blood of lung cancer patients
Cancer Res. 58: 2761-2765, 1998
Chen BM, Chan LY, Wang SM, Wu MF, Chern JW and Roffler SR
Cure of malignant ascites and generation of protective immunity by monoclonal antibody-targeted activation of a glucuronide prodrug in rats
Int. J. Cancer, 73: 392-402, 1997
Cheng TL, Chen BM, Chan LY, Wu PW, Chern JW, and Roffler SR
Poly(ethylene glycol) modification of beta-glucuronidase-antibody conjugates for solid-tumor therapy by targeted activation of glucuronide prodrugs
Cancer Immun. Immunother., 44: 305-315, 1997
Jiang SY, Shyu RY, Huang HF, Tang HS, Young TH, Roffler SR, Chiou YS, and Yeh MY
Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis
Brit. J. Cancer, 75: 928-933, 1997
Shyu RY, Jiang SY, Huang SL, Chang TC, Wu, KL, Roffler SR, and Yeh MY
Growth regulation by all-trans-retinoic acid and retinoic acid receptor messanger ribonucleic acids expression in gastric cancer cells
Eur. J. Cancer 31A: 237-243, 1995
Shen AY Hwang MH, Roffler SR, and Chen CF
Cytotoxicty and antimicrobial activity of some naphthol derivatives
Arch. Pharm. (Weinheim) 328: 197-201, 1995
Roffler SR, Chan J and Yeh MY
Potentiation of radioimmunotherapy by inhibition of topoisomerase
Cancer Research 54: 1276-1285, 1994
Wang JJ, Roffler SR, Chou HH, Yin FY and Yin CS
Characterization of mullerian inhibiting substance binding on cervical carcinoma cells demonstrated by immunohistochemistry
Tissue and Cell, 26: 467-476, 1994
Wang SM, Chern JW, Yeh MY, Ng JC, Tung E, and Roffler SR
Specific activation of glucuronide prodrugs by antibody-targeted enzyme conjugates for cancer therapy
Cancer Res. 52: 4484-4491, 1992
Wang JJ, Roffler SR, Yu MH, Cheng CH, Yin CS, and Yeh MY
Characterization of a tumor associated antigen present on human cervical carcinoma cells by lectin binding and electron microscopy
Histochem. 97: 453-461, 1992
Yu MH, Roffler SR, Hwang WS, Chen WL and Yeh MY
In vivo localization of human cervical carcinoma xenografts by radiolabeled monoclonal antibody
Gynecol. Oncol. 47: 93-101, 1992
Yeh MY, Roffler SR, and Yu MH
Doxorubicin-monoclonal antibody conjugate for therapy of human cervical carcinoma
Int. J. Cancer 51: 274-282, 1992
Roffler SR, Wang SM, Chern JW, Yeh MY, and Tung E
Anti-neoplastic glucuronide prodrug treatment of human tumor cells targerted with a monoclonal antibody-enzyme conjugate
Biochem. Pharmacol., 42: 2062-2065, 1991
Yu MH, Roffler SR, Liu HS, Yin CS, and Yeh MY
Flow cytometric analysis of monoclonal antibody reactivity and specificity against human cervical carcinoma
Chinese J. Obstet. Gynaecol., 30: 90-95, 1991
Roffler SR, Yu MH, Chen BM, Hsu CJ, and Yeh MY
Therapy of human cervical carcinoma with monoclonal antibody - Psuedomonas exotoxin conjugates
Cancer Res., 51: 4001-4007, 1991
Roffler SR, Yu MH, Chen MR, Chen SC, Hsu CT, and Yeh MY
New monoclonal antibody against human cervical carcinoma with diagnostic and therapeutic potential
Chinese J. Microbiol. Immunol. 24: 19-29, 1991
Roffler SR, Randolph TW, Miller DA, Blanch HW, and Prausnitz JM
Extractive Bioconversions with non-aqueous solvents
Bioprocess Technol. 11: 133-172, 1991
Roffler SR, Blanch HW and Wilke CR
Design and mathematical description of differential contactors used in extractive fermentation
Biotechnol. Bioeng. 32: 192-204, 1988
Roffler SR, Blanch HW and Wilke CR
In- situ extractive fermentation of acetone and butanol
Biotechnol. Bioeng. 31: 135-143, 1988
Roffler SR, Blanch HW and Wilke CR
Extractive fermentation of acetone and butanol: Process design and economic evaluation
Biotechnol. Progress 3: 131-140, 1987
Roffler SR, Blanch HW and Wilke CR
In-situ recovery of butanol during fermentation. II. Fed-batch extractive fermentation
Bioprocess Eng. 2: 181-190, 1987
Roffler SR, Blanch HW and Wilke CR
In-situ recovery of butanol during fermentation. I. Batch extractive fermentation
Bioprocess Eng. 2: 1-12, 1986
Roffler SR, Blanch HW, and Wilke C
In-situ recovery of fermentation products
Trends in Biotechnol. 2: 129-136, 1984