Dr. Cheng, Ching-Feng 's publons link picture


  • 02-2789-9135 (Lab) (Room No: N706)

  • Integrative Physiology
  • Molecular Cardiology
  • Metabolic syndrome

Education and Positions:
  • M.D., Taipei Medical University

    Post Doctoral Fellowship, Kenneth R. Chien Lab, UCSD, U.S.A

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Green tea epigallocatechin gallate inhibits preadipocyte growth via the microRNA-let-7a/HMGA2 signaling pathway

Dr. Cheng, Ching-Feng
Molecular Nutrition & Food Research, Feb 24, 2023




Scope: This study investigated the effect of epigallocatechin gallate (EGCG) on white and beige preadipocyte growth and explored the involvement of the miR-let-7a/HMGA2 pathway.

Methods and results: 3T3-L1 and D12 cells were treated with EGCG. The effect of EGCG on cell proliferation and viability was evaluated, as well as microRNA (miRNA)-related signaling pathways. EGCG inhibited 3T3-L1 and D12 preadipocyte growth, upregulated miR-let-7a expression, and downregulated high-mobility group AT-hook 2 (HMGA2) mRNA and protein levels in a time- and dose-dependent manner. In addition, overexpression of miR-let-7a significantly inhibited the growth of 3T3-L1 and D12 cells and decreased HMGA2 mRNA and protein levels. MiR-let-7a inhibitor antagonized the inhibitory effects of EGCG on the number and viability of 3T3-L1 and D12 cells. Furthermore, miR-let-7a inhibitor reversed the EGCG-induced increase in miR-let-7a expression levels and decrease in HMGA2 mRNA and protein levels. HMGA2 overexpression induced an increase in cell number and viability and antagonized EGCG-suppressed cell growth and HMGA2 expression in 3T3-L1 and D12 preadipocytes.

Conclusion: EGCG inhibits the growth of 3T3-L1 and D12 preadipocytes by modulating the miR-let-7a and HMGA2 pathways. This article is protected by copyright. All rights reserved.