Dr. Tang, Tang K. 's publons link picture

Dr. Tang, Tang K.

Vice President, Academia Sinica
Distinguished Research Fellow
Academician, Academia Sinica
  • 02-27899156(lab) (Lab) (Room No: 301)
  • 02-26523901(o)
  • 02-27829143 (Fax)

  • Centrosome and Cilia Biogenesis
  • Mitosis
  • Neural Stem Cell Division

Education and Positions:
  • Ph.D. Human Genetics, Yale University

Highlight Detail

Loss of human microcephaly CPAP in developing mouse brain

Dr. Tang, Tang K.
J Cell Sci, Jun 24, 2020

Primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by small brain size with mental retardation. CPAP/CENPJ, a known microcephaly gene, plays a key role in centriole biogenesis. Here, we generated a previously unreported conditional knockout allele in the mouse Cpap gene. Our results showed that conditional Cpap deletion preferentially induces formation of monopolar spindles in radial glia progenitors (RGPs) and causes robust apoptosis that severely disrupts embryonic brains. Interestingly, microcephalic brains with reduced apoptosis are detected in the conditional Cpap gene-deleted mice, which lose only one allele of p53, while simultaneous removal of p53 and Cpap rescues RGPs death. Furthermore, Cpap deletion leads to cilia loss, RGPs mislocalization, junctional integrity disruption, massive heterotopia, and severe cerebellar hypoplasia. Together, these findings indicate that complete CPAP loss leads to severe and complex phenotypes in developing mouse brain, and provide new insights into the causes of MCPH.