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Dr. Huang, Yi-Shuian

Research Fellow
Division Chief
  • 02-2789-9174 (Lab) (Room No: N703)
  • 02-2652-3523 (Office)

  • Translational Control/ RNA 轉譯調控
  • Cap modification/ RNA-cap 修飾調控
  • Molecular & Cellular Neuroscience/ 分子與細胞神經生物學

Education and Positions:
  • Ph.D. University of Texas Southwestern Medical Center at Dallas

Highlight Detail

NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress.

Dr. Huang, Yi-Shuian
Nucl. Acids Res., Oct 07, 2015

Non-selenocysteine-containing phospholipid hydroperoxide glutathione peroxidase (NPGPx or GPx7) is an oxidative stress sensor that modulates the antioxidative activity of its target proteins through intermolecular disulfide bond formation. Given NPGPx's role in protecting cells from oxidative damage, identification of the oxidative stress-induced protein complexes, which forms with key stress factors, may offer novel insight into intracellular reactive oxygen species homeostasis. Here, we show that NPGPx forms a disulfide bond with the translational regulator cytoplasmic polyadenylation element-binding protein 2 (CPEB2) that results in negative regulation of hypoxia-inducible factor 1-alpha (HIF-1α) RNA translation. In NPGPx-proficient cells, high oxidative stress that disrupts this bonding compromises the association of CPEB2 with HIF-1α RNA, leading to elevated HIF-1α RNA translation. NPGPx-deficient cells, in contrast, demonstrate increased HIF-1α RNA translation under normoxia with both impaired induction of HIF-1α synthesis and blunted HIF-1α-programmed transcription following oxidative stress. Together, these results reveal a molecular mechanism for how NPGPx mediates CPEB2-controlled HIF-1α RNA translation in a redox-sensitive manner.