Dr. Wu, Jer-Yuarn 's publons link picture

Dr. Wu, Jer-Yuarn

Research Fellow
  • 02-27899075 (Lab) (Room No: N520)
  • 02-27824066 (Fax)

  • Human Genetics
  • Pharmacogenetics
  • Genome Medicine

Education and Positions:
  • Ph.D, Duke University, USA

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Novel susceptibility gene for nonfamilial hypokalemic periodic paralysis.

Dr. Wu, Jer-Yuarn
Neurology, Mar 02, 2016

Objective: To identify susceptibility genes to nonfamilial hypokalemic periodic paralysis (hypoKPP) consisting of thyrotoxic periodic paralysis (TPP) and sporadic periodic paralysis (SPP) and explore the potential pathogenic mechanisms.

Methods: We enrolled patients with nonfamilial hypoKPP not carrying mutations in CACNA1S, SCN4A, KCNJ18, or KCNJ2 and conducted genome-wide association analyses comparing 77 patients with TPP and 32 patients with SPP with 1,730 controls in a Han Chinese population in Taiwan. Replication was performed using an independent Han Chinese cohort of 50 patients with TPP, 22 patients with SPP, and 376 controls.

Results: We identified 4 single nucleotide polymorphisms (rs312692, rs312736, rs992072, rs393743) located about 100 Kb downstream of KCNJ2 on chromosome 17q24.3 associated with both TPP and SPP reaching genome-wide significance (p < 9 × 10−8). rs312736 was mapped to CTD-2378E21.1, a lincRNA, and direct sequencing revealed an exon variant rs312732 (risk allele A) highly associated with both TPP (p = 1.81 × 10−12; odds ratio [OR] 3.22 [95% confidence interval (CI) 2.36–4.40]) and SPP (p = 8.6 × 10−12; OR 5.4 [95% CI 3.17–9.18]). Overexpression of C (normal allele) CTD-2378E21.1 in C2C12 skeletal muscle cell, but not A (risk allele) CTD-2378E21.1, showed significantly decreased Kcnj2 expression, indicating A-type CTD-2378E21.1 has lost the ability to regulate Kcnj2.

Conclusions: Our study reveals a shared genetic predisposition between TPP and SPP. CTD-2378E21.1 is a novel disease-associated gene for both TPP and SPP and may negatively regulate KCNJ2 expression. These findings provide new insights into the pathogenesis of nonfamilial hypoKPP.