徵求有志從事跨領域生醫/神經科學研究的新成員，與我們一起解構神經傳導的細胞及分子網絡。

這是一個高度跨領域的研究主題，我們結合化學、生物工程、生物物理、以及神經科學的概念與技術，發展創新的工具及方法，進而運用這些科技精準調控或探測複雜的神經傳導現象。我們已開發了一系列光遺傳/光藥理學工具，目前正建立各式實驗系統進行生物測試與應用。

We develop chemical and genetically-encoded tools for neuroscience applications.  This is a highly cross-disciplinary research field.  We integrate concepts and techniques in chemistry, bioengineering, biophysics, and neurobiology, aiming to enable precise manipulation and/or probing of complex neurotransmission in the brain. We have developed a series of novel optogenetic/photopharmacological tools, and we are establishing various biological systems to explore potential applications of these tools.

生命科學、神經科學、醫藥或生醫工程相關科系學士/碩士畢業，熟悉電生理、小鼠實驗、或細胞生化/蛋白質體實驗 (符合一項即可)。歡迎有興趣開發optogenetic/chemogenetic tools於神經科學之應用者提出申請。

若有advanced microscopy (confocal, two-photon, tissue clearing/expansion, etc.)，protein design/modeling或bioinformatics的相關經驗也歡迎申請，但這些經驗不是必要條件。

Prospective candidates wishing to employ optogenetic/chemogenetic tools in neurobiology and holding a  M.S. or B.S. degree in life sciences, neuroscience, biomedical sciences, or bioengineering are strongly encouraged to apply.  We are looking for a new member who is experienced in one of the following: electrophysiology, mouse surgery, mouse in vivo experiments, or neural biochemical/proteomic analysis.  However, other candidates that have experience in advanced microscopy (confocal, two-photon, tissue clearing/expansion, etc.), protein design/modeling, or bioinformatics are also welcomed.

細胞免疫相關機制探討

有小鼠實驗經驗佳

具分子細胞生物學背景

1.配合計畫執行

2.動物實驗與分生實驗操作

3.支援一般性實驗室事務

1.生物生醫, 生物科技, 化學, 化工相關領域學/碩士畢業

2.需熟悉分生實驗, 腸道菌, 幹細胞研究, 化學合成等且不懼動物實驗

3.具團隊精神，有耐心、細心負責

4.願意學習並對科學有熱誠者為佳

OVERVIEW

The Cheng lab is looking for an enthusiastic and excellent Research Technician to join our dynamic and competitive group in human immunology research in the Institute of Biomedical Science at Academia Sinica, Taiwan. Our research focuses on deep-profiling human virus-specific T cells during viral infection, vaccination, or virus-associated cancer by using highly multiplexed combinatorial peptide-MHC tetramer staining, mass cytometry, and single-cell sequencing methods (Cheng et al., 2019. Science Immunology; Cheng et al., 2021. Immunity). We are interested in dissecting the different arms of human adaptive immune responses, and characterize their functional and cellular heterogeneity, particularly tissue-resident memory T cells and follicular helper T cells. We are currently working on chronic hepatitis B infection (HBV), HBV-associated hepatocellular carcinoma and liver disease, and SARS-CoV-2 infection and vaccination.

RESPONSIBILITIES

1. Learn nuances of mass cytometry experiment, maintenance, and the preparation of reagents.
2. Produce proteins required for highly multiplexed combinatorial peptide-MHC tetramer staining.
3. Use single-cell sequencing methods to further profile human antigen-specific T cells.
4. Perform high-dimensional data analysis.
5. Carry out high quality research under the supervision of Dr. Yang Cheng or senior member in the lab, and support other lab members’ projects.
6. Lab management.

QUALIFICATIONS

Minimum qualifications:

• Passionate in science
• Bachelor’s degree in related scientific fields
• Cell and tissue culture, experience in common techniques in molecular and cellular biology
• Great organization and communication (verbal and writing) skills
• Team player with great interpersonal characteristics
• Experience in flow cytometry

Preferred qualifications (candidates who have the following criteria will be considered as favored):

• Experience in handling and processing human clinical samples
• Certified phlebotomist
• Experience in molecular cloning, and protein production and purification
• Experience in TCR sequencing
• Experience in FACS sorting
• Experience in spectral flow cytometry
• Experience in R (or python)

We are investigating a new kind of liposome invented in our lab for the treatment of cancer. The research involves learning how to make liposomes, load different cancer drugs into the liposomes, how to characterize the liposomes, and investigate the in vitro and in vivo anticancer activity of the liposomes.

The goal of this research is to form a company. This project involves also learning how to start a business in the Biotechnology Research Park of Academia Sinica. The successful candidate should be highly motivated to carry our translation research with the goal of commercializing the product.

BS or MS degreee in Biology or Chemistry related fields

Cancer immunotherapy 研究

DC-T 細胞免疫相關機制探討

有小鼠實驗經驗佳

具分子細胞生物學背景

癌症免疫治療:
1.建立人類免疫系統小鼠
2.發展癌症疫苗與細胞免疫治療

基因治療:
1.研發腺相關病毒載體(AAV)大量生產
2.開發基因治療用的AAV載體

研究地點在 生醫所和國家生技研究園區，

研究內容以產品開發為導向，將接受初階商業課程訓練，未來有技轉和創業機會

生命科學相關科系學士或碩士/博士畢業

• (1)利用基因轉殖鼠，及分子生物學，生物化學，動物行為學，計算科學的方法進行研究;
• (2)研究幼年型「肌強直症」大腦發育遲緩及退化的分子機轉;
• (3) 研究「注意力不足及過動症」的致病機轉。

1. 生命科學相關科系畢業之學士或碩士, 具操作小鼠實驗經驗尤佳。
2. 有一個學習的熱忱，(i) 想學習如何利用基因轉殖鼠進行研究; (ii) 對神經發育及神經退化性疾病的研究有興趣; (iii) 想學習如何利用分子生物, 生物化學, 動物行為學的方法研究導致疾病的分子致病機轉。
3. 並且願意和有經驗的人一起學習，共同成長。

配合計劃執行，協助核心設施一般行政業務與推廣

具生命科學相關科系學位