Dr. Lee, Sho Tone

Research Fellow

  • Drug Resistance
  • Immunology

Education and Positions:
  • Ph.D. University of Manitoba Canada

As a member of the Division of Infectious Diseases, our laboratory focuses our effort in Leishmaniasis on three topics: (1) study of the interaction of Leishmania parasite with host mononuclear phagocytes through ligand-receptor relationship; (2) using ICAM molecule in the form of DNA or protein to immunize mice to investigate the host immune response against Leishmania. (3) study of the mechanism of genesis of drug resistance and gene amplification in protozoan Leishmania parasites.

(1) An intercellular adhesive molecule from Leishmania (ICAM) cloned and expressed is found to involve in the interaction between the parasite and its host macrophage.Identification of receptor for ICAM molecule and the pathogenesis induced by such an interaction is under investigation. (2) Leishmania infection attracts neutrophils to the site of infection.Recent finding indicated that elastase of neutrophil interacts with TLR4 of macrophage to produce TNFa for elimination of Leishmania.ICAM immunization recruited large amount of neutrophils to the peritoneal cavity.We are now investigating the role of ICAM and recruited neutrophils in the response of the host against Leishmania parasite.(3) In drug resistance, a genome wide search of the genes activated during drug resistance induction in order to understand the mechanisms behind drug resistance and gene amplification.Subtractive hybridization and proteomic approaches are used for a genome wide comparison between the wildtype and the drug-resistant Leishmania variants.

Journal 22 Book 0

  1. J. Y. Hsu, Y. C. Lin, S. C. Chiang and S. T. Lee Divergence of trypanothione-dependent tryparedoxin cascade into cytosolic and mitochondrial pathways in arsenite-resistant variants of Leishmania amazonensis Mol Biochem Parasitol 157(2), 193-204 (2008) [JCR] [WOS]
  2. J. R. Wang, S. T. Lee, W. H. Juan, W. L. Chuang, S. I. Hung, W. H. Chung and H. S. Hong Indigenous leishmaniasis in Taiwan: report of a case Int J Dermatol 47(1), 40-43 (2008) [JCR] [WOS]
  3. Y. C. Lin, J. Y. Hsu, J. H. Shu, Y. Chi, S. C. Chiang and S. T. Lee Two distinct arsenite-resistant variants of Leishmania amazonensis take different routes to achieve resistance as revealed by comparative transcriptomics Mol Biochem Parasitol 162(1), 16-31 (2008) [JCR] [WOS]
  4. S. C. Chiang, C. C. Chang, Y. C. Lin, H. P. Ng and S. T. Lee Loop 1 structure of the leishmanial ICAM-L molecule is crucial for parasite binding and infection of host macrophages Int J Parasitol 37(8-9), 1001-1011 (2007) [JCR] [WOS]
  5. O. I. Kuzmenok, G. A. Dvoryanchikov, E. N. Ponomareva, A. A. Goncharov, I. K. Fomin, S. T. Lee, P. R. Sanberg and M. P. Potapnev Myasthenia gravis accompanied by thymomas not related to foamy virus genome in belarusian\'s patients Int J Neurosci 117(11), 1603-1610 (2007) [JCR] [WOS]
  6. Y. C. Lin, J. Y. Hsu, S. C. Chiang and S. T. Lee Distinct overexpression of cytosolic and mitochondrial tryparedoxin peroxidases results in preferential detoxification of different oxidants in arsenite-resistant Leishmania amazonensis with and without DNA amplification Mol Biochem Parasitol 142, 66-75 (2005) [JCR] [WOS]
  7. J. C. Chen, F. M. Ho, P. D. Lee Chao, C. P. Chen, K.C.G. Jeng, H. B. Hsu, S. T. Lee, W. T. Wu and W. W. Lin Inhibition of iNOS gene expression by quercetin is mediated by the inhibition of IkappaB kinase, nuclear factor-kappa B and STAT1, and depends on heme oxygenase-1 induction in mouse BV-2 microglia Eur J Pharmacol 521, 9-20 (2005) [JCR] [WOS]
  8. O. I. Kuzmenok, S. C. Chiang, Y. C. Lin and S. T. Lee Retardation of cell cycle progression of macrophages from G1 to S phase by ICAM-L from Leishmania Int J Parasitol 35, 1547-1555 (2005) [JCR] [WOS]
  9. C. T. Chen, Y. C. Yi, S. C. Chiang, K. C. Liu, Y. L. Lin and S. T. Lee Selection of immunodominant fragments from envelope gene for vaccine against Japanese encephalitis virus in DNA priming - protein boosting protocols Microb Pathog 38, 53-62 (2005) [JCR] [WOS]
  10. S. C. Chang, O. Kuzmenok, S. C. Cheng and S. T. Lee An improved method for detection of Leishmania amastigotes by an antibody probe against the small subunit of leishmanial ribonucleotide reductase Parasitol Res 94, 243-245 (2004) [JCR] [WOS]