Ph.D. University of Manitoba Canada
As a member of the Division of Infectious Diseases, our laboratory focuses our effort in Leishmaniasis on three topics: (1) study of the interaction of Leishmania parasite with host mononuclear phagocytes through ligand-receptor relationship; (2) using ICAM molecule in the form of DNA or protein to immunize mice to investigate the host immune response against Leishmania. (3) study of the mechanism of genesis of drug resistance and gene amplification in protozoan Leishmania parasites.
(1) An intercellular adhesive molecule from Leishmania (ICAM) cloned and expressed is found to involve in the interaction between the parasite and its host macrophage.Identification of receptor for ICAM molecule and the pathogenesis induced by such an interaction is under investigation. (2) Leishmania infection attracts neutrophils to the site of infection.Recent finding indicated that elastase of neutrophil interacts with TLR4 of macrophage to produce TNFa for elimination of Leishmania.ICAM immunization recruited large amount of neutrophils to the peritoneal cavity.We are now investigating the role of ICAM and recruited neutrophils in the response of the host against Leishmania parasite.(3) In drug resistance, a genome wide search of the genes activated during drug resistance induction in order to understand the mechanisms behind drug resistance and gene amplification.Subtractive hybridization and proteomic approaches are used for a genome wide comparison between the wildtype and the drug-resistant Leishmania variants.