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Dr. Lee, Yungling Leo

Research Fellow
  • 02-27899132 (Lab) (Room No: N343)
  • 02-26523013 (Office)
  • 02-27829142 (Fax)

Specialty:

1. Adaptive Immunity

2. Omics and Precision Medicine

3. Antigen Presenting Cells

4. Vaccine Development


Education and Positions:
  • M.D. National Taiwan University

    Ph.D. National Cheng Kung University


Clinical Immunology Lab: linking innate cells to adaptive immunity

 

T cell Regulation in Inflammatory Diseases

1. We have demonstrated that UVB-exposed Treg cells contributed to the alleviation of inflammatory skin in a murine model of OVA-induced skin inflammation under UVB irradiation. When depleting Treg cells in Foxp3DTR/GFP mice by the administration of diphtheria toxin, we found UVB irradiation could not suppress OVA-induced skin inflammation. In addition, we found that LAG-3 expression was significantly increased in UVB-exposed Treg cells.

 

2. Cytokine signaling between keratinocytes and innate cells results in CD4/CD8/Treg cell infiltration of psoriasis lesions. We applied single-cell mass cytometry (Cytometry Time-Of-Flight, CyTOF) to investigate function of Treg cells in the peripheral blood of patients with psoriasis. We will also apply single-cell RNA sequencing (scRNA-seq) to dissect the differential pattern among skin cells of patients before and after UVB therapy.

 

DC-based Cancer Immunotherapy

1. Emerging evidence suggests the promoting role of aromatic hydrocarbon receptor (AhR) in the initiation, promotion, progression, invasion, and metastasis of cancer cells. AhR-CD11c-conditional KO mice were generated and we found tumor growth were significantly reduced in conditional KO mice. It was also noted that activation of AhR can induce PD-L1 level on dendritic cell (DC) and Treg recruitment.

 

2. We employed phage display to select anti-CD11c peptide and AhR-neutralizing single-chain fragment variable (ScFv). PEGylated peptide is inserted into surface of liposome and the transcript of neutralizing ScFv is packaged in the inner space of liposome. Liposome coats acid-labile PEG-PGA-HX to prevent systemic peptidase and accelerating endosomal escape in acidic tumor microenvironment. Finally, we implemented the humanized ASID mice to investigate the efficiency of liposomal drugs. The DC-targeted cancer treatment remodels the tumor microenvironment and reduces side effects.

 

Our Team
Team photo

  1. Yeh CY, Su SH, Tan YF, Tsai TF, Liang PH, Kelel M, Weng HJ, Hsiao YP, Lu CH, Tsai CH, Lee CH, Clausen BE, Liu FT, Lee YL* PD-L1 Enhanced by cis-Urocanic Acid on Langerhans Cells Inhibits Vγ4+ γδT17 Cells in Imiquimod-Induced Skin Inflammation JOURNAL OF INVESTIGATIVE DERMATOLOGY 143, 1449-1460 (2023-08) [JCR] [WOS]
  2. Tsai CH, Lai AC, Lin YC, Chi PY, Chen YC, Yang YH, Chen CH, Shen SY, Hwang TL, Su MW, Hsu IL, Huang YC, Maitland-van der Zee AH, McGeachie MJ, Tantisira KG, Chang YJ, Lee YL* Neutrophil extracellular trap production and CCL4L2 expression influence corticosteroid response in asthma SCIENCE TRANSLATIONAL MEDICINE 15(699), eadf3843 (2023-06) [JCR] [WOS]
  3. Chen CH, Lee YL, Wu MH, Chen PJ, Wei TS, Tseng CI, Chen WJ Prenatal household incense burning, air purifiers and cord blood IgE CLINICAL AND EXPERIMENTAL ALLERGY 53(4), 474-478 (2023-04) [JCR] [WOS]
  4. Tsai MC, Lee YL*, Chen YC* Association of the consumption of common drinks with early puberty in both sexes FRONTIERS IN PUBLIC HEALTH 10, 854477 (2022-12) [JCR] [WOS]
  5. Chen YC, Huang YT, Pan WH, Lee YL* Mediators linking obesity to childhood asthma PEDIATRIC ALLERGY AND IMMUNOLOGY e13859 (2022-10) [JCR] [WOS]
  6. Chen YC, Su MW, Brumpton BM, Lee YL* Investigating obesity-related risk factors for childhood asthma PEDIATRIC ALLERGY AND IMMUNOLOGY e13710, e13710 (2022-01) [JCR] [WOS]
  7. Chen CH, Lee YL, Wu MH, Chen PJ, Wei TS, Tseng CI, Chen WJ Prenatal antioxidant-enriched and pro-oxidant-contained food, IL4 and IL13 pathway genes, and cord blood IgE. Scientific reports 12(1), 2884 (2022) [JCR] [WOS]
  8. Huang L, Wang ST, Kuo HP, Delclaux C, Jensen ME, Wood LG, Costa D, Nowakowski D, Oliveira PD, Chen YC, Chen YC*, Lee YL* Effects of Obesity on Pulmonary Function Considering the Transition from Obstructive to Restrictive Pattern from Childhood to Young Adulthood OBESITY REVIEWS e13327, e13327 (2021-12) [JCR] [WOS]
  9. Chen YC, Kuo HP, Hsia SM, Wu HT, Pan WH, Lee YL* Life course body mass index through childhood and young adulthood and risks of asthma and pulmonary function impairment PEDIATRIC PULMONOLOGY 56, 849-857 (2021-05) [JCR] [WOS]
  10. Kelel M, Yang RB, Tsai TF, Liang PH, Wu FY, Huang YT, Yang MF, Hsiao YP, Wang LF, Tu CF, Liu FT, Lee YL* FUT8 Remodeling of EGFR Regulates Epidermal Keratinocyte Proliferation during Psoriasis Development Journal of Investigative Dermatology 141, 512-522 (2021-03) [JCR] [WOS]