Dr. Mou, Yun
Associate Research Fellow
- 2789-9035 (Lab) (Room No: N721)
- 2652-3009 (Office)
-
Specialty:
- Computational protein design
- Antibody engineering
- Immunotherapy
- Cancer proteomics
- CRISPR screening
Education and Positions:
- B.S. National Taiwan University
- M.S. National Taiwan University
- Ph.D. California Institute of Technology
- Postdoc. University of California, San Francisco
Our laboratory devotes on developing genetically engineerable biologics for cancer therapy, especially immunotherapy. We focus on four types of therapeutic biologics: proteins, virus (gene therapy), bio-nanoparticles, and bacteria. For protein therapy, we employed state-of-the-art techniques, including computational protein design, directed evolution, phage/yeast display, to improve the properties of anti-tumor proteins, such as antibodies/nanobodies, cytokines, CRISPR/Cas proteins, etc. For virus-based gene therapy, we develop novel vehicles by deep engineering of bacteriophage. Bacteriophage has several advantages over the traditional human virus for gene therapy, including cheap production, high yield, and designed tropism to human cells. For bio-nanoparticle therapy, we utilize the outer membrane vesicles (OMVs), which is a highly immunogenic nanoparticle generated by bacteria. OMVs show great tumor-targeting ability and anti-tumor activity, and can be genetically modified by plasmid transformation. For bacterial therapy, several bacteria, including E. coli, can target and colonize in tumors. We exploit bacteria as a live factory for constantly secreting therapeutic payloads into tumor microenvironment.
We have successfully achieved complete remission in murine tumor models using several modalities of our therapies. While continuously improving our drug designs, we will expand our endeavors to more tumor models in mice and potentially other high mammals.
Journal 39
Book 0
Journal 39 Book 0
- Tang Wen-Hung, Wang Shi-Han, Wang Chiu-Feng, Mou Yun, Lin Min-Guan, Hsiao Chwan-Deng, Liao You-Di The lipid components of high-density lipoproteins (HDL) are essential for the binding and transportation of antimicrobial peptides in human serum. Scientific Reports (2022) [JCR] [WOS]
- Hu Che-Wei, Chang You-Chiun, Liu Cheng-Hao, Yu Yao-An, Mou Kurt Yun Development of a TNF-α-mediated Trojan Horse for bacteria-based cancer therapy. Molecular Therapy 30(7), 2522-2536 (2022) [JCR] [WOS]
- Lin Wen-Ching, Tang Hao-Cheng, Wang Han Ying, Kao Chia-Yi, Chang You-Chiun, Li Athena Hsu, Yang Shi-Bing, Mou Kurt Yun Fragment-Directed Random Mutagenesis by the Reverse Kunkel Method. ACS Synthetic Biology 11(4), 1658-1668 (2022) [JCR] [WOS]
- Wang Han Ying, Chang You-Chiun, Hu Che-Wei, Kao Chia-Yi, Yu Yao-An, Lim See-Khai, Mou Kurt Yun Development of a Novel Cytokine Vehicle Using Filamentous Phage Display for Colorectal Cancer Treatment. ACS Synthetic Biology 10(8), 2087-2095 (2021) [JCR] [WOS]
- Chen Wentao, Mou Kurt Yun, Solomon Paige, Aggarwal Rahul, Leung Kevin K., Wells James A. Large remodeling of the Myc-induced cell surface proteome in B cells and prostate cells creates new opportunities for immunotherapy. Proceedings of the National Academy of Sciences 118(4), e2018861118 (2021) [JCR] [WOS]
- Liu Cheng-Hao, Chang Jen-Hsuan, Chang You-Chiun, (Mou Kurt Yun*) Treatment of murine colitis by Saccharomyces boulardii secreting atrial natriuretic peptide. Journal of Molecular Medicine 98(12),1675-1687 (2020-12) [JCR] [WOS]
- Huang Meng‐Sen, Chang Jen‐Hsuan, Lin Wen‐Ching, Cheng Yu‐Hsiang, Li Fu‐An, Suen Ching‐Shu, Hwang Ming‐Jing, Chang Chung‐ke*, (Mou Kurt Yun*) SLC38A2 Overexpression Induces a Cancer‐like Metabolic Profile and Cooperates with SLC1A5 in Pan‐cancer Prognosis. Chemistry – An Asian Journal 15(22),3861-3872 (2020-11) [JCR] [WOS]
- Zhou Xin X., Bracken Colton J., Zhang Kaihua, Zhou Jie, (Mou Yun), Wang Lei, Cheng Yifan, Leung Kevin K., Wells James A. Targeting Phosphotyrosine in Native Proteins with Conditional, Bispecific Antibody Traps. Journal of the American Chemical Society 142(41),17703-17713 (2020-10) [JCR] [WOS]
- Liu Cheng-Hao, Chien Ming-Jou, Chang You-Chiun, Cheng Yu-Hsiang, Li Fu-An, (Mou Kurt Yun*) Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome. Journal of Proteome Research 19(3) 1109-1118 (2020-03) [JCR] [WOS]
- Chang You-Chiun, Kao Chia-Yi, Tang Hao-Cheng, Huang Meng-Sen, (Mou Kurt Yun*) Direct Antibody Isolation on Cells Using Affinity-Tag-Guided Proximity Selection. Biochemistry 59(440), 4285-4293 (2020) [JCR] [WOS]
- POSTDOC -
Lim, See Khai
- RESEARCH ASSOCIATES -
Lin, WenChing
Hu, Che-Wei
Pan, Yi-Chung
- STUDENTS -
Kao, Chiayi
Chang, You-Chiun
Liu, Cheng-Hao
Lee, Tzu Chieh
Huang, Sin-Wei
Yu, Yao-An
Chen, Mei-Yi
Wu, Wan-Ci
Lien, Wan-Ju
Cheng, Ting-Wei
Hsiao, Yi-Hsiang
- VISITOR -
Wu, Shania
- ALUMNI -
Tang, Hao-Cheng
Ho, Chia-Lo
Chien, Ruby
Chang, Jen-Hsuan
Huang, Meng-Sen
Wang, Han-Ying
