Dr. Tarn, Woan-Yuh 's publons link picture

Dr. Tarn, Woan-Yuh

Distinguished Research Fellow
  • 02-27899015 (Lab) (Room No: N223)
  • 02-26523052
  • 02-27829142 (Fax)



  1. mRNA splicing in neuron
  2. translational control in cancer
  3. long non-coding RNA

Education and Positions:
  • Ph.D. National Tsing Hua University
    Postdoc Assoc. Yale University

Highlight Detail

DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation

Dr. Tarn, Woan-Yuh
iScience, Sep 24, 2021



Using antibody arrays, we found that the RNA helicase DDX3 modulates the expression of secreted signaling factors in oral squamous cell carcinoma (OSCC) cells. Ribo-seq analysis confirmed amphiregulin (AREG) as a translational target of DDX3. AREG exerts important biological functions in cancer, including promoting cell migration and paracrine effects of OSCC cells and reprogramming the tumor microenvironment (TME) of OSCC in mice. DDX3-mediated translational control of AREG involves its 3′-untranslated region. Proteomics identified the signal recognition particle (SRP) as an unprecedented interacting partner of DDX3. DDX3 and SRP54 were located near the endoplasmic reticulum, regulated the expression of a common set of secreted factors, and were essential for targeting AREG mRNA to membrane-bound polyribosomes. Finally, OSCC-associated mutant DDX3 increased the expression of AREG, emphasizing the role of DDX3 in tumor progression via SRP-dependent, endoplasmic reticulum-associated translation. Therefore, pharmacological targeting of DDX3 may inhibit the tumor-promoting functions of the TME.