Dr. Yang, Ruey-Bing (Ray)'s publons link picture

Dr. Yang, Ruey-Bing (Ray)

Acting Director
Research Fellow
  • (02) 2789-9063 (Lab) (Room No: N427)
  • (02) 2652-3943
  • (02) 2785-8847 (Fax)


Signal transduction

Receptor biology

Vascular biology

Education and Positions:
  • Ph.D., University of Texas Southwestern Medical Center at Dallas

    Postdoctoral Fellow, Genentech, Inc.

Highlight Detail

Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis

Dr. Yang, Ruey-Bing (Ray)
Arteriosclerosis, Thrombosis, and Vascular Biology, Nov 10, 2016

Approach and Results—SCUBE2 lentiviral overexpression in human ECs increased and short hairpin RNA knockdown inhibited VEGF-induced EC growth and capillary-like network formation on Matrigel. Like VEGF, endothelial SCUBE2 was upregulated by hypoxia-inducible factor-1α at both mRNA and protein levels. EC-specific Scube2 knockout mice were not defective in vascular development but showed impaired VEGF-induced neovascularization in implanted Matrigel plugs and recovery of blood flow after hind-limb ischemia. Coimmunoprecipitation and ligand-binding assays showed that SCUBE2 forms a complex with VEGF and VEGFR2, thus acting as a coreceptor to facilitate VEGF binding and augment VEGFR2 signal activity. SCUBE2 knockdown or genetic knockout suppressed and its overexpression promoted the VEGF-induced activation of downstream proangiogenic and proliferating signals, including VEGFR2 phosphorylation and mitogen-activated protein kinase or AKT activation.

Conclusions—Endothelial SCUBE2 may be a novel coreceptor for VEGFR2 and potentiate VEGF-induced signaling in adult angiogenesis.