3.3-biotin is available for use as a detection agent. This antibody works very well as a detection antibody in ELISA.
6.3-biotin binds to the PEG backbone with greater affinity than 3.3. The binding epitope (~ 750 Da PEG) is competed by Tween 20 or detergents that contain ethylene oxide backbones, so alternative detergents such as CHAPs should be used with 6.3-biotin.
r33G-biotin binds to the PEG backbone with greater affinity than 3.3. Binding to PEG is resistant to Tween 20 or detergents that contain ethylene oxide backbones, so these detergents can be used with r33G-biotin.
15-2b-biotin binds to methoxy PEG (mPEG) with high affinity and works great for the detection of mPEG-modified nanoparticles.
AGP3-biotin can be used to capture and detect PEGylated compounds.
AGP4-biotin can be immobilized in streptavidin-coated plates to capture PEGylated compounds.
rAGP6-biotin is useful when high affinity capture of PEGylated compounds is desired.