Dr. Roffler, Steve R. 's orcid link picture Dr. Roffler, Steve R. 's publons link picture

Dr. Roffler, Steve R.

Distinguished Research Fellow
Division Chief
  • 02-2789-9152 (Lab) (Room No: N233)
  • 02-2652-3079 (Office)
  • 02-2782-9142 (Fax)
Specialty:
  1. Antibody Engineering
  2. Directed Molecular Evolution
  3. Prodrugs
  4. Anti-PEG antibodies
  5. Nanomedicines
Education and Positions:

Ph.D. University of California, Berkeley

1. Antibody engineering: We are engineering and investigating bispecific antibodies to deliver nanomedicines into cancer cells for improved anticancer selectivity and efficacy.    

2. Nanomedicine: New approaches to stably load and retain hydrophobic drugs in liposomal nanocarriers are under development.    

3. Directed molecular evolution: We perform directed molecular evolution of human enzymes for antibody-directed enzyme prodrug therapy. 

 4. Prodrug therapy: Anticancer prodrugs that are selectively activated in the tumor microenvironment are under investigation to improve the selectivity and therapeutic efficacy of cancer treatment. 

5. Anti-PEG antibodies: We develop antibodies to assay PEGylated drugs in human serum and are extending these studies to investigating the impact of pre-existing and induced anti-PEG antibodies on the therapeutic efficacy and safety of PEGylated medicines.

Our Team
Team photo

Journal 180 Book 2

  1. Liu Y, Mori T, Ito Y, Kuroki K, Hayashi S, Kohda D, Shimizu T, Ishida T, Roffler SR, Kaneko MK, Kato Y, Arimori T, Teramoto T, Takemura K, Ishibashi K, Katayama Y, Maenaka K, Kakuta Y, Kitao A, Mori T The strategy used by naive anti-PEG antibodies to capture flexible and featureless PEG chains.. JOURNAL OF CONTROLLED RELEASE 380, 396-403 (2025-02) [JCR] [WOS]
  2. Meng JL, Dong ZX, Chen YR, Lin MH, Liu YC, Roffler SR, Lin WW, Chang CY, Tzou SC, Cheng TL, Huang HC, Li ZQ, Lin YC, Su YC pH-Responsive polyethylene glycol engagers for enhanced brain delivery of PEGylated nanomedicine to Treat Glioblastoma.. ACS NANO 19(1), 307-321 (2025-01) [JCR] [WOS]
  3. Ho KW, Liu YL, Huang BC, Hong ST, Yang SH, Liao TY, Liu ES, Chen YT, Huang YZ, Leu YL, Chen CY, Chen BM, Roffler SR, Cheng TL Targeted internalization and activation of glycosidic switch liposomes by a biological macromolecule mPEGxEphA2 increases therapeutic efficacy against lung cancer.. International journal of biological macromolecules 300, 140138 (2025-01) [JCR] [WOS]
  4. Chen Bing-Mae, Chen Even, Lin Yi-Chen, Tran Trieu Thi My, Turjeman Keren, Yang Shih-Hung, Cheng Tian-Lu, Barenholz Yechezkel, Roffler Steve R. Liposomes with Low Levels of Grafted Poly(ethylene glycol) Remain Susceptible to Destabilization by Anti-Poly(ethylene glycol) Antibodies. ACS Nano epub ahead of print, NA-NA (2024-08) [JCR] [WOS]
  5. Tseng Yi-Han, Lin Hsuan-Pei, Lin Sung-Yao, Chen Bing-Mae, Vo Thanh Nguyet Nguyen, Yang Shih-Hung, Lin Yi-Chen, Prijovic Zeljko, Czosseck Andreas, Leu Yu-Lin, Roffler Steve R Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Journal of Controlled Release 369, 179-198 (2024-05) [JCR] [WOS]
  6. Liu YL, Liao TY, Ho KW, Liu ES, Huang BC, Hong ST, Hsieh YC, Chang MS, Wu BT, Chen FM, Roffler SR, Chen CY, Yang YC, Cheng TL Impact of Pre-existing Anti-polyethylene Glycol Antibodies on the Pharmacokinetics and Efficacy of a COVID-19 mRNA Vaccine (Comirnaty) In Vivo.. Biomaterials research 28, 0112 (2024) [JCR] [WOS]
  7. Tran Trieu TM, Roffler Steve R Interactions between nanoparticle corona proteins and the immune system. Current Opinion in Biotechnology 84, 103010 (2023-12) [JCR] [WOS]
  8. Chen Huei-Jen, Cheng Yi-An, Chen Yu-Tung, Li Chia-Ching, Huang Bo-Cheng, Hong Shih-Ting, Chen I.-Ju, Ho Kai-Wen, Chen Chiao-Yun, Chen Fang-Ming, Wang Jaw-Yuan, Roffler Steve R., Cheng Tian-Lu, Wu Dung-Ho Targeting and internalizing PEGylated nanodrugs to enhance the therapeutic efficacy of hematologic malignancies by anti-PEG bispecific antibody (mPEG × CD20). Cancer Nanotechnology 14(1), 78 (2023-10) [JCR] [WOS]
  9. Beishenaliev Adilet, Loke Yean Leng, Goh Sook Jing, Geo Hui Nee, Mugila Malar, Misran Misni, Chung Lip Yong, Kiew Lik Voon, Roffler Steve, Teo Yin Yin Bispecific antibodies for targeted delivery of anti-cancer therapeutic agents: A review. Journal of Controlled Release 359, 268-286 (2023-07) [JCR] [WOS]
  10. Chen Wei-An, Chang Deng-Yuan, Chen Bing-Mae, Lin Yi-Chen, Barenholz Yechezekel, Roffler Steve R. Antibodies against Poly(ethylene glycol) Activate Innate Immune Cells and Induce Hypersensitivity Reactions to PEGylated Nanomedicines. ACS Nano 17(6), 5757-5772 (2023-03) [JCR] [WOS]
- More Publications-

- POSTDOC -
member
Tseng, Yi-Han
- RESEARCH ASSOCIATES -
member
Wu, Penny
Yang, Seymour
Yang, Seymour
- ALUMNI -
member
Tang, Hsiyang
member
Chang, Deng-Yuan(Peter)
Lin, Ronald
Lin, Ronald

2017    MOST Outstanding Research Award

2018    14th Tien Te Lee Outstanding Award (14th永信李天德醫藥科技)

2018    25th TECO Technology Foundation’s Outstanding Achievement Award in Biomedical Technology (第二十五屆東元獎)

2019    Ministry of Economic Affairs 6th National Industrial Innovation Award (第6屆經濟部國家產業創新獎個人類獎項)

2020    MOST Outstanding Research Award

2022    Outstanding Research Award of the Taiwan Nanomedicine Society

2022    Ministry of Education’s 63rd Annual Academic Award (63屆教育部學術獎)